Molecular Epidemiology of Chicken Anemia Virus (CAV) in Southeastern Chinese Live Birds Markets

Ducatez, Mariette; Chen, H.; Guan, Yi

doi:10.1637/1933-5334(2008)3[e7:meocav]2.0.co;2

Cited by 4 publications

(7 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The overall phylogenetic pattern and clustering of different CAV isolates based on the partial VP1 gene in this study was similar to previous one based on complete sequence of VP1 gene [ 24 ] or the entire CAV genome [ 25 ] for the common CAV isolates considered in all the three studies. This suggests that relationships of different CAV isolates can be determined on the basis of partial sequence of VP1 gene due to the fact that most of the amino acid substitutions in comparisons between isolates lies in VP1 gene and more specifically on the N-terminal half of VP1 gene.…”

Section: Discussionsupporting

confidence: 84%

“…This is similar to the result by Ducatez et al . [ 24 ] where they indicated a very slow CAV virus evolution at amino acid level corresponding to a strong negative selection (0.04 to 0.20) of VP1 gene in China and worldwide. However, in this study, we found that one of the isolates has omega value of 0.50 meanwhile five out of 12 isolates have omega value between 0.25 to 0.35 and the rest with omega value of 0.07 to 0.11 (Table 2 ).…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Detection and characterization of chicken anemia virus from commercial broiler breeder chickens

Hailemariam

Omar

Hair-Bejo

et al. 2008

Virol J

View full text Add to dashboard Cite

Background: Chicken anemia virus (CAV) is the causative agent of chicken infectious anemia (CIA). Study on the type of CAV isolates present and their genetic diversity, transmission to their progeny and level of protection afforded in the breeder farms is lacking in Malaysia. Hence, the present study was aimed to detect CAV from commercial broiler breeder farms and characterize CAV positive samples based on sequence and phylogenetic analysis of partial VP1 gene.

show abstract

Section: Discussionsupporting

confidence: 84%

Section: Discussionmentioning

confidence: 99%

Detection and characterization of chicken anemia virus from commercial broiler breeder chickens

Hailemariam

Omar

Hair-Bejo

et al. 2008

Virol J

View full text Add to dashboard Cite

show abstract

“…Genetic characterization of numerous Lao CoV and CAV strains from chickens and ducks was attempted. Almost all Lao strains were closely related to strains from Asia, but, as observed previously (Ducatez et al, 2008;Valastro et al, 2016), the phylogenetic analyses revealed no clear geographic clustering. Most interestingly, the partial genomes from virus strains detected in ducks and chickens were closely related, which further highlights the high risk of cross-species transmission in this setting.…”

Section: Discussionsupporting

confidence: 47%

“…We therefore applied a generic PCR allowing the detection of CoV strains from all genera. The highly variable VP1 gene of CAV, which is also a major genetic determinant of virulence, has been the target of many studies assessing the genetic diversity of CAV strains (Renshaw et al, 1996;Yamaguchi et al, 2001;Ducatez et al, 2006;Ducatez et al, 2008;Hailemariam et al, 2008;Snoeck et al, 2012;Li et al, 2017). In addition, all samples were tested for NDV by real-time RT-PCR (Mia et al, 2008) targetting the matrix gene.…”

Section: Virus Screening and Sequencingmentioning

confidence: 99%

Cross-species transmission of poultry pathogens in backyard farms: ducks as carriers of chicken viruses

et al. 2019

View full text Add to dashboard Cite

In backyard farms of Lao People's Democratic Republic, mixed-species rearing of poultry is a breeding-ground for cross-species transmission. Here, the epidemiology of viruses circulating among backyard poultry in Vientiane Province was assessed to guide future control strategies. Oral/tracheal and cloacal swabs, collected from 605 poultry (308 ducks, 297 chickens) between 2011 and 2015, were screened by PCR for Newcastle disease virus (NDV), coronavirus (CoV) and chicken anaemia virus (CAV). Chicken sera were screened for anti-NDV antibodies by ELISA. Statistical and phylogenetic analyses revealed transmission patterns and relationships. Closely related strains co-circulated in chickens and ducks. While CoV RNA was detected in oral/tracheal swabs of 9.3% of the chickens and 2.4% of the ducks, rates were higher in faecal swabs of both species (27.3% and 48.2%). RNA of infectious bronchitis virus (IBV) and duck CoV was found in faecal swabs of chickens (19.7% and 7.1%) and ducks (4.1% and 44.1%). Moreover, DNA of the generally chicken-specific CAV was detected in oral/tracheal swabs of chickens (18.1%) and, sporadically, of ducks (2.4%). Despite serological evidence of NDV circulation or vaccination (86.9%), NDV RNA was not detected. We found a high prevalence and indication for cross-species transmission of different CoV strains in backyard poultry. Interestingly, ducks served as biological, or at least mechanical, carriers of viral strains closely related not only to IBV, but also to CAV. Bird containment and poultry species separation could be first steps to avoid cross-species transmission and emergence of novel strains with broad host range and enhanced pathogenicity. RESEARCH HIGHLIGHTS. High rates of avian viruses were detected by PCR in backyard poultry from Lao PDR.. Diverse coronavirus and chicken anemia virus strains co-circulated.. Phylogenetic analyses suggested virus transmission between chickens and ducks.. Serological evidence of Newcastle disease was found, but viral RNA was not detected.

show abstract

“…CAV was detected by PCR in the examined broiler flock and the amplified PCR product, which represent partial VP1 gene, was sequenced and the CAV strain was designated as CLEVB-Zag2 with accession number KX171350 at the gene bank. Phylogenetic analysis at the amino acids and nucleotide level of VP1 classify CLEVB-Zag2 CAV under group III (Genotype III) of the CAV in agreement with Islam et al (2002), Ducatez et al (2006Ducatez et al ( , 2008, Craig et al (2009), Kim et al (2010) and Snoeck et al (2012).…”

Section: Discussionmentioning

confidence: 68%

Pathogenicity and immunosuppressive potential of an Egyptian field isolate (2015) of the chicken anemia virus (CAV) in chickens

Nassif

Latif

El-Sayed

et al. 2017

Journal of Veterinary Medical Research

View full text Add to dashboard Cite

Chicken anemia virus (CAV) is the causative agent of chicken infectious anemia (CIA). Studying CAV isolates in Egypt and their genetic diversity and its potential role in vaccination failure recently noticed in broiler flocks, is lacking in Egypt. So, the present study aimed to characterize CAV isolate-collected from a commercial broiler flock suffered from severe anemia and high mortality based on sequence and phylogenetic analysis of partial VP1 gene as well as to study pathogenicity and immunosuppressive potential in one day-old SPF chicks. The CAV isolate proved to be positive by PCR. The PCR product was sequenced and submitted to the gene bank under the accession number KX171350 and the CAV strain was designated as CLEVB-Zag2. Phylogenetic analysis at the nucleotide and amino acids level classify CLEVB-Zag2 CAV under group III (genotype III) of CAVs. On the other hand, the CLEVB-Zag2 CAV was found to be highly pathogenic for the experimentally inoculated SPF-chicks showing depression, severe anemia in almost all chicks in two infected groups beginning at the 7th day post infection (PI) and reached the peak of severity at the 14th day (hematocrit value, hemoglobin conc. and RBCs counts) are significantly reduced in chicks of the infected group. Blue wings were observed in few chicks in each infected group at the 14th day PI. Macroscopic lesions consisting of subcutaneous and muscular hemorrhages are observed with pale bone marrow, significant atrophy of thymus, spleen and bursa, hepatomegally with mottled liver and paleness of the carcasses were detected 7 days PI Those findings were evident and increased in severity until the day 14 PI. Concerning the CLEVB-Zag2 CAV, it was found to be highly immunosuppressive in the infected SPFchicks vaccinated with a commercial potent inactivated H5N1 vaccine as manifested by a significant reduction (protection 50%). The variance in the titer of the shieded challenge H5N1 virus was 1.45 log10 and the mean HI titer at the 4th week post vaccination was 1.5 log2 compared with the non-infected vaccinated group where these values were 90%, 2.35 log10 and 5.3 log2; respectively. In conclusion, the present study revealed that the CLEVB-Zag2 CAV isolate is highly pathogenic and immunosuppressive.

show abstract

Molecular Epidemiology of Chicken Anemia Virus (CAV) in Southeastern Chinese Live Birds Markets

Abstract: Reg BioOne Complete (complete.BioOne.org) is a full-text database of 200 subscribed and open-access titles in the biological, ecological, and environmental sciences published by nonprofit societies, associations, museums, institutions, and presses.

Cited by 4 publications

References 0 publications

Detection and characterization of chicken anemia virus from commercial broiler breeder chickens

Detection and characterization of chicken anemia virus from commercial broiler breeder chickens

Cross-species transmission of poultry pathogens in backyard farms: ducks as carriers of chicken viruses

Pathogenicity and immunosuppressive potential of an Egyptian field isolate (2015) of the chicken anemia virus (CAV) in chickens

Contact Info

Product

Resources

About