Molecular epidemiology of Escherichia coli producing extended-spectrum β-lactamases in Lugo (Spain): dissemination of clone O25b:H4-ST131 producing CTX-M-15

Blanco, Miguel; Alonso, Marı́a Pilar; Nicolas–Chanoine, Marie–Hélène; Dahbi, Ghizlane; Mora, Azucena; Blanco, Jesús E.; López, Cecilia; Cortés, Pilar; Llagostera, Montserrat; Leflon‐Guibout, Véronique; Puentes, Beatriz; Mamani, Rosalía; Herrera, Alexandra; Coira, Amparo; Garcı́a-Garrote, Fernando; Pita, J.; Blanco, Jorge

doi:10.1093/jac/dkp122

Cited by 119 publications

(87 citation statements)

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“…We identified two intestinal AIEC strains and one extraintestinal AIEC strain, all of which were of the O25:H4 serotype, that belonged to the emerging and virulent clonal group ST131 (6,14,26,31,42). Currently, this clonal pathotype is the most prevalent among human ExPEC strains.…”

Section: Discussionmentioning

confidence: 99%

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

et al. 2009

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Adherent-invasive Escherichia coli (AIEC) pathovar strains, which are associated with Crohn's disease, share many genetic and phenotypic features with extraintestinal pathogenic E. coli (ExPEC) strains, but little is known about the level of genetic similarity between the two pathovars. We aimed to determine the frequency of strains with the "AIEC phenotype" among a collection of ExPEC strains and to further search for a common phylogenetic origin for the intestinal and extraintestinal AIEC strains. The adhesion, invasion, and intramacrophage replication capabilities (AIEC phenotype) of 63 ExPEC strains were determined. Correlations between virulence genotype and AIEC phenotype and between intestinal/extraintestinal origin, serotype, and phylogroup were evaluated for the 63 ExPEC and 23 intestinal AIEC strains. Phylogenetic relationships between extraintestinal and intestinal AIEC strains were determined using multilocus sequence typing (MLST) and pulsed-field gel electrophoresis. Only four (6.35%) ExPEC strains, belonging to the O6:H1, O83:H1, and O25:H4 serotypes, were classified as having an AIEC phenotype. These strains were found to be genetically related to some intestinal AIEC strains of the same serotypes as revealed by MLST. No particular virulence gene sets correlated with the intestinal/extraintestinal origin of the strains or with the AIEC phenotype, whereas the gene sets did correlate with the serogroup. We identified two intestinal AIEC strains and one extraintestinal AIEC strain belonging to the O25:H4 serotype that also belonged to the emerging and virulent clonal group ST131. In conclusion, the ExPEC and AIEC pathovars share similar virulence gene sets, and certain strains are phylogenetically related. However, the majority of ExPEC strains did not behave like AIEC strains, thus confirming that the AIEC pathovar possesses virulence-specific features that, to date, are detectable only phenotypically.Members of the Enterobacteriaceae family, especially Escherichia coli, have been repeatedly suggested to play a role in the origin and/or perpetuation of Crohn's disease (CD). In part, this suggestion was based on the higher abundance of this bacterium in CD patients than in control subjects (4,10,20,23,28,29,32,41,48,51). Although considerable effort has been devoted to the search for intestinal pathogenic E. coli strains associated with CD, to date none of the six previously described pathovars (27) has been implicated in this condition. observed that E. coli strains with adhesion and invasion properties colonized the ileal mucosae of CD patients more frequently than those of control subjects. Darfeuille-Michaud et al. further characterized these strains and proposed a new potential E. coli pathovar associated with CD, which was designated adherent-invasive E. coli (AIEC) (10). The implication of AIEC in CD is becoming increasingly relevant because several independent studies from different countries have reported a higher prevalence of invasive E. coli in CD patients (4,17,33,34,47).The main chara...

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Section: Discussionmentioning

confidence: 99%

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

et al. 2009

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“…This clonal group is an emerging human pathogen in many parts of the world, including continental Europe (3,33), the United Kingdom (28), Turkey (52), Korea (30), Canada (36,38), and the United States (18,19). Its association with bla CTX-M-15 , which encodes the CTX-M-15 extended-spectrum ␤-lactamase (ESBL), has also influenced the recent pandemic spread of extended-spectrum cephalosporin resistance (3,4,18,28,29,34,44).…”

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Commonality among Fluoroquinolone-Resistant Sequence Type ST131 Extraintestinal Escherichia coli Isolates from Humans and Companion Animals in Australia

Platell

Cobbold

Johnson

et al. 2011

Antimicrob Agents Chemother

112

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Escherichia coli sequence type 131 (ST131), an emergent multidrug-resistant extraintestinal pathogen, has spread epidemically among humans and was recently isolated from companion animals. To assess for humancompanion animal commonality among ST131 isolates, 214 fluoroquinolone-resistant extraintestinal E. coli isolates (205 from humans, 9 from companion animals) from diagnostic laboratories in Australia, provisionally identified as ST131 by PCR, selectively underwent PCR-based O typing and bla CTX-M-15 detection. A subset then underwent multilocus sequence typing (MLST), pulsed-field gel electrophoresis (PFGE) analysis, extended virulence genotyping, antimicrobial susceptibility testing, and fluoroquinolone resistance genotyping. All isolates were O25b positive, except for two O16 isolates and one O157 isolate, which (along with six O25b-positive isolates) were confirmed by MLST to be ST131. Only 12% of isolates (25 human, 1 canine) exhibited bla CTX-M-15 . PFGE analysis of 20 randomly selected human and all 9 companion animal isolates showed multiple instances of >94% profile similarity across host species; 12 isolates (6 human, 6 companion animal) represented pulsotype 968, the most prevalent ST131 pulsotype in North America (representing 23% of a large ST131 reference collection). Virulence gene and antimicrobial resistance profiles differed minimally, without host species specificity. The analyzed ST131 isolates also exhibited a conserved, host species-independent pattern of chromosomal fluoroquinolone resistance mutations. However, eight (89%) companion animal isolates, versus two (10%) human isolates, possessed the plasmid-borne qnrB gene (P < 0.001). This extensive across-species strain commonality, plus the similarities between Australian and non-Australian ST131 isolates, suggest that ST131 isolates are exchanged between humans and companion animals both within Australia and intercontinentally.

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“…Oteo et al recently reported that ESBLproducing E. coli isolates in Spain usually correspond to phylogroup A (40%), followed by group B1 (28%), group D (18%), and finally, group B2 (14%) (27). B2 is mainly associated with extraintestinal pathogenic strains from the community and is frequently overrepresented in ESBL studies (2,33). In our collection, B2 is represented in both community (all from urine samples) and hospital (two from urine, two from blood, and one from respiratory secretions) origins.…”

Section: Discussionmentioning

confidence: 99%

Population Analysis and Epidemiological Features of Inhibitor-Resistant-TEM-β-Lactamase-Producing Escherichia coli Isolates from both Community and Hospital Settings in Madrid, Spain

et al. 2010

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Punctual mutations in the TEM-1 or TEM-2 gene may lead to inhibitor-resistant-TEM (IRT) ␤-lactamases with resistance to ␤-lactam-␤-lactamase inhibitor combinations and susceptibility to cephalosporins. The aim of this work was to analyze the current epidemiology of IRT ␤-lactamases in contemporary clinical Escherichia coli. Isolates were prospectively collected in our hospital (2007 and 2008) from both outpatients (59.8%) and hospitalized patients (40.2%). The genetic relationships of the isolates were determined by XbaI pulsed-field gel electrophoresis, multilocus sequence typing, and phylogenetic group analysis. IRT genes were sequenced and located by hybridization, and the incompatibility group of the plasmids was determined. From a total of 3,556 E. coli isolates recovered during the study period, 152 (4.3%) showed reduced susceptibility to amoxicillin-clavulanate, with 18 of them producing IRT enzymes (0.5%). These were mostly recovered from urine (77.8%). A high degree of IRT diversity was detected (TEM-30, -32, -33, -34, -36, -37, -40, and -54), and the isolates were clonally unrelated but were mostly associated with phylogenetic group B2 (55.5%). In 12 out of 16 (75%) isolates, the bla IRT gene was plasmid located and transferred by conjugation in 9 of them, whereas chromosomal localization was demonstrated in 4 isolates (25%). The sizes of the plasmids ranged from 40 kb (IncN) to 100 kb (IncFII, IncFI/FIIA), and they showed different restriction patterns by restriction fragment length polymorphism analysis. Unlike extended-spectrum ␤-lactamase producers, the frequency of IRT producers remains low in both community and hospital settings, with most of them causing urinary tract infections. Although bla IRT genes are mainly associated with plasmids, they can be also located in the chromosome. Despite this situation, clonal expansion and/or gene dispersion was not observed, denoting the independent emergence of these enzymes.Resistance to ␤-lactam-␤-lactamase inhibitor combinations in Escherichia coli may be due to different mechanisms, including TEM-1 penicillinase hyperproduction, constitutive AmpC overproduction or plasmid AmpC production, OXA-type ␤-lactamase production, permeability deficiencies involving OmpF and/or OmpC porins, inhibitor-resistant TEM (IRT)-and complex mutant TEM (CMT)-like ß-lactamase production, and more recently, carbapenemase production (4).IRT enzymes comprise a group of plasmid-encoding variants of TEM-1 and TEM-2 with decreased affinities for amino-, carboxy-, and ureidopenicillins and altered interaction with class A ß-lactamase inhibitors (6). IRT-producing isolates remain susceptible to cephalosporins, cephamycins, carbapenems, and in most cases, piperacillin-tazobactam. They are usually resistant to ampicillin-sulbactam and intermediate or resistant to amoxicillin-clavulanate combinations. IRT enzymes have previously been reported in different organisms, such as E. coli, Klebsiella spp., Enterobacter cloacae, Proteus mirabilis, Citrobacter freundii, and Shigella sonnei (4)...

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Molecular epidemiology of Escherichia coli producing extended-spectrum β-lactamases in Lugo (Spain): dissemination of clone O25b:H4-ST131 producing CTX-M-15

Cited by 119 publications

References 20 publications

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

Similarity and Divergence among Adherent-Invasive Escherichia coli and Extraintestinal Pathogenic E. coli Strains

Commonality among Fluoroquinolone-Resistant Sequence Type ST131 Extraintestinal Escherichia coli Isolates from Humans and Companion Animals in Australia

Population Analysis and Epidemiological Features of Inhibitor-Resistant-TEM-β-Lactamase-Producing Escherichia coli Isolates from both Community and Hospital Settings in Madrid, Spain

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