Molecular epidemiology of HCV infection among acute and chronic liver disease patients in Kolkata, India

Chaudhuri, Swapna; Das, Sayan; Chowdhury, Abhijit; Santra, Amal; Bhattacharya, Sanjay; Naik, T. N.

doi:10.1016/j.jcv.2004.07.003

Cited by 29 publications

(22 citation statements)

References 33 publications

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“…HCV genotype 3 was major circulating genotype (87.65%) followed by genotype 1 in our study. Our results were similar to the data obtained in other HCV populations (such as haemophilia and haemodialysis) where HCV genotype 3 was the dominant infecting strain29.…”

Section: Discussionsupporting

confidence: 91%

Post-transfusion hepatitis C virus infection among β-thalassaemic individuals with associated clinical parameters

Biswas

Firdaus

Saha

et al. 2018

Indian Journal of Medical Research

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Background & objectives:Multiple transfusions in β-thalassaemia patients undergoing regular transfusion regimen are at a risk of developing transfusion transmitted infections, including hepatitis C virus (HCV). The present study was conducted to investigate the association of HCV viraemia and genotype with clinical parameters in HCV seroreactive β-thalassaemic individuals.Methods:A total of 172 HCV seroreactive β-thalassaemic individuals aged between 2-35 yr with at least 25 units of blood transfusion were catagorized into four groups (2-12 yr, group 1; 13-19 yr, group 2; 20-29 yr, group 3; 30-35 yr, group 4). Aged matched control samples (n=87; β-thalassaemics without HCV infection) were also included. HCV RNA was detected by nested reverse transcriptase polymerase chain reaction (RT-PCR) based on 5’ UTR of HCV genome, viral load was determined by real-time RT-PCR. Nested RT-PCR amplified partial core region was used for DNA sequencing. Liver function parameters [serum total bilirubin, alanine aminotransferase (ALT) and aspartate aminotransferase (AST)] were also determined.Results:Of the 172 HCV seroreactive individuals, 59.30 per cent (n=102) were HCV RNA positive. HCV viral load ranged from 173 to 32.04×10[5] IU/ml; 87.65 per cent were infected with HCV genotype 3. Liver enzymes, such as ALT, AST and serum total bilirubin were significantly elevated in all age groups compared to control groups. Serum ferritin levels were found to be high in all individuals, but 16.27 per cent of HCV-infected individuals with >10,000 IU/ml viral load also showed high ferritin levels (>1500 μg/l) where the majority of them were infected with HCV genotype 3.Interpretation & conclusions:HCV genotype 3 was the major circulating genotype among β-thalassaemia patients in this region. Our findings indicated an association between HCV replication and hepatic iron load and also highlighted the need for sensitive quantitative RT-PCR-based detection of HCV RNA in the high risk population.

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Section: Discussionsupporting

confidence: 91%

Post-transfusion hepatitis C virus infection among β-thalassaemic individuals with associated clinical parameters

Biswas

Firdaus

Saha

et al. 2018

Indian Journal of Medical Research

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“…Genotype of the virus does not appear to infl uence disease presentation or severity of disease but has been identifi ed as a major predictor of response to antiviral therapy. Most of the reported studies from India seem to suggest a north south divide, wherein genotype 3 predominates in the north, east and west India, whereas genotype 1 is commoner in south India ( Valliammai et al 1995;Panigrahi et al 1996;Amarapurkar et al 2001;Chowdhury et al 2003;Lole et al 2003;Raghuraman et al 2003;Singh and Sarin 2004;Chaudhuri et al 2005;Hissar et al 2006;Khaja et al 2006). This fi nding is further substantiated by a report published from one of the leading virology laboratories in India .…”

Section: Molecular Biologymentioning

confidence: 89%

Hepatitis C in India

Mukhopadhya

2008

J Biosci

103

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Hepatitis C is an emerging infection in India and an important pathogen causing liver disease in India.The high risk of chronicity of this blood-borne infection and its association with hepatocellular carcinoma underscores its public health importance. Blood transfusion and unsafe therapeutic interventions by infected needles are two preventable modalities of spread of hepatitis C infection. In addition, risk factor modification by reducing the number of intravenous drug users will help curtail the prevalence of this infection. This review summarizes the extent, nature and implications of this relatively new pathogen in causing disease in India.

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“…HCV genotyping study was considered because of its great epidemiological significance and has not been adequately ascertained in Indian context among HIV population. HCV genotype 3 is the most prevalent form in Northern and Eastern part of India [37,38]. In Western region significant difference in HCV genotype 3 and genotype 1 is not observed [36,38,39] but in Southern India, HCV genotype 1 is more predominant than Genotype 3 [34,36,38].…”

Section: Discussionmentioning

confidence: 99%

Recent pattern of Co-infection amongst HIV seropositive individuals in tertiary care hospital, kolkata

Saha

Firdaus

Santra

et al. 2011

Virol J

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BackgroundOpportunistic Infections (OIs) and co-infections are the major cause of deaths amongst HIV infected individuals and this mostly depends upon the risk factors, type of exposure and geographic region. The commonest types of infections reported are tuberculosis, chronic diarrhoea, oral candidiasis, herpes simplex virus-2, cytomegalovirus, hepatitis B virus and hepatitis C virus. Due to the scarcity of OIs data available from this region, we had designed a study to determine the frequency of different OIs amongst HIV seropositive patients.MethodsAnalysis of the different spectrum of OIs/Co-infections were carried out with 204 HIV sero-positive patients (142 males and 62 females) who visited the HIV/AIDS Apex Clinic in a tertiary care hospital from March 2006 to March 2009. The CD4+ count was estimated using FACS Calibur, the routine smear test, serology, nested RT-PCR and DNA sequencing were carried out to determine the different OIs.ResultsIn this study, HIV seropositive patients were mostly from middle age group (31-40 yrs) with CD4+ counts in majority of symptomatic AIDS patients below 200 cells/mm3. The common co-infections/opportunistic infections were OC (53.43%), CD (47.05%), HSV-2 (36.76%), TB (35.29%), CMV (26.96%), HBV (15.19%) and HCV (7.35%). Dual infections, like HSV-2 & CMV (15.38%), HSV-2 & TB (14.61%), HSV-2 & oral candidiasis (24.61%) and CMV & oral candidiasis (14.61%) were significant in follow-up patients. Triple infections were also common e.g., TB, CD, OC infection occurring frequently in about 14.21% of the study population. Multiple infections like OC, TB, CD amongst the viral co-infected patients with HSV-2, HCV, CMV and HBV are also reported in this study. The genotyping analysis of the HCV co-infected HIV individuals shows that two belonged to HCV genotype 1 and 8 belonged to genotype 3.ConclusionsA wide spectrum of OIs were observed amongst HIV-infected patients in the HIV/AIDS Apex Clinic. Oral candidiasis, CD, CMV and HSV-2, were the common OIs in those patients. This study aims to provide a clearer picture regarding infections occurring amongst HIV seropositive individuals so that the scientific findings could be translated into sustainable prevention programmes and improved public health policies.Trial registrationNone

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Molecular epidemiology of HCV infection among acute and chronic liver disease patients in Kolkata, India

Cited by 29 publications

References 33 publications

Post-transfusion hepatitis C virus infection among β-thalassaemic individuals with associated clinical parameters

Post-transfusion hepatitis C virus infection among β-thalassaemic individuals with associated clinical parameters

Hepatitis C in India

Recent pattern of Co-infection amongst HIV seropositive individuals in tertiary care hospital, kolkata

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