Molecular epidemiology of <i>Candida</i> species isolated from urine at an intensive care unit

Ergon, Mahmut Cem; Gülay, Zeynep

doi:10.1111/j.1439-0507.2004.01086.x

Cited by 20 publications

(17 citation statements)

References 20 publications

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“…The reasons for the rising incidence of C. parapsilosis candidemia are not completely known, although indwelling venous catheters and parenteral nutrition have been recognized as specific risk factors [4,17,18]. Most experimental studies have indicated that the adherence of C. parapsilosis to acrylic surfaces is greater than that of C. albicans [19].…”

Section: Discussionmentioning

confidence: 99%

Molecular investigation of a fungemia outbreak due to Candida parapsilosis in an intensive care unit

et al. 2008

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We investigated a nosocomial cluster of four Candida parapsilosis fungemia episodes that occurred in a neurological intensive care unit over a two-week period. The four infected patients had received parenteral nutrition through central lines, and all four had catheter-related candidemia. All of the isolates were susceptible to all of the antifungals tested, including amphotericin B, fluconazole, voriconazole, and caspofungin. They had strictly related fingerprints, based on randomly amplified polymorphic DNA analysis. Additional DNA sequencing data revealed that they were same strain. Although no isolate of Candida parapsilosis was recovered from other clinical, surveillance, or environmental samples, nosocomial spread of this yeast ceased, following the reinforcement of infection-control measures. Candida parapsilosis may require an intravascular foreign body to cause fungemia, but this outbreak shows that it can be transmitted nosocomially and can cause epidemics.

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Section: Discussionmentioning

confidence: 99%

Molecular investigation of a fungemia outbreak due to Candida parapsilosis in an intensive care unit

et al. 2008

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“…The investigation performed by Szweda et al (2013) revealed 26 genotypes among 54 C. glabrata isolates, but it is noteworthy that the authors used only one primer in the study. Additionally, Ergon and Gülay (2005) observed a lower degree of genetic diversity among C. glabrata strains. The authors suggested that it is possible that primers used in the study were not appropriate for the typing of non-albicans species (Ergon & Gülay 2005).…”

Section: Discussionmentioning

confidence: 91%

“…Additionally, Ergon and Gülay (2005) observed a lower degree of genetic diversity among C. glabrata strains. The authors suggested that it is possible that primers used in the study were not appropriate for the typing of non-albicans species (Ergon & Gülay 2005). Some authors highlighted that the strains classified as identical by one primer are not always classified as belonging to the same cluster when analysed by another primer (Bonfim-Mendonça et al 2013).…”

Section: Discussionmentioning

confidence: 91%

Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland

Paluchowska

Tokarczyk

Bogusz³

et al. 2014

Mem. Inst. Oswaldo Cruz

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Over the last decades, Candida spp have been responsible for an increasing number of infections, especially in patients requiring intensive care. Knowledge of local epidemiology and analysis of the spread of these pathogens is important in understanding and controlling their transmission. The aim of this study was to evaluate the genetic diversity of 31 Candida albicans and 17 Candida glabrata isolates recovered from intensive care unit patients from the tertiary hospital in Krakow between 2011-2012. The strains were typed by random amplified polymorphic DNA (RAPD) polymerase chain reaction using five primers (CD16AS, HP1247, ERIC-2, OPE-3 and OPE-18). The results of the present investigation revealed a high degree of genetic diversity among the isolates. No clonal relationship was found among the C. albicans strains, whereas two C. glabrata isolates were identical. The source of Candida infection appeared to be mostly endogenous; however, the presence of two clonal C. glabrata strains suggested the possibility of cross-transmission of these pathogens. Our study confirmed the high discriminatory power of the RAPD technique in the molecular typing of Candida clinical isolates. This method may be applied to the evaluation of transmission routes of pathogenic fungi on a local level.

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“…Coefficients of genetic similarity are indicated in the horizontal line ( S AB ). Vertical line indicates the threshold S AB = 0.81, separating the related and unrelated groupings 20 …”

Section: Resultsmentioning

confidence: 99%

“…Candida species are an important cause of systemic nosocomial infections, being responsible for the major documented fungal infections. 1,20 Epidemiological elucidation of this type of infection and determination of the sources of hospital-acquired C. albicans infections relies mainly on typing methods, which are based on phenotypical or molecular characteristics of the isolates. However, stability of these characteristics after in vitro storage is essential to assure typing methodological validity.…”

Section: Discussionmentioning

confidence: 99%

Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods

Bacelo

Costa

Ferreira

et al. 2009

Mycoses

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Typing methods to evaluate isolates in relation to their phenotypical and molecular characteristics are essential in epidemiological studies. In this study, Candida albicans biotypes were determined before and after storage in order to verify their stability. Twenty C. albicans isolates were typed by Randomly Amplified Polymorphic DNA (RAPD), production of phospholipase and proteinase exoenzymes (enzymotyping) and morphotyping before and after 180 days of storage in Sabouraud dextrose agar (SDA) and sterilised distilled water. Before the storage, 19 RAPD patterns, two enzymotypes and eight morphotypes were identified. The fragment patterns obtained by RAPD, on the one hand, were not significantly altered after storage. On the other hand, the majority of the isolates changed their enzymotype and morphotype after storage. RAPD typing provided the better discriminatory index (DI) among isolates (DI = 0.995) and maintained the profile identified, thereby confirming its utility in epidemiological surveys. Based on the low reproducibility observed after storage in SDA and distilled water by morphotyping (DI = 0.853) and enzymotyping (DI = 0.521), the use of these techniques is not recommended on stored isolates.

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Molecular epidemiology of Candida species isolated from urine at an intensive care unit

Cited by 20 publications

References 20 publications

Molecular investigation of a fungemia outbreak due to Candida parapsilosis in an intensive care unit

Molecular investigation of a fungemia outbreak due to Candida parapsilosis in an intensive care unit

Molecular epidemiology of Candida albicans and Candida glabrata strains isolated from intensive care unit patients in Poland

Biotype stability of Candida albicans isolates after culture storage determined by randomly amplified polymorphic DNA and phenotypical methods

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