Molecular epidemiology of Leptospira interrogans in Rattus norvegicus in Hanoi, Vietnam

Koizumi, Nobuo; Miura, Koryo; Sanai, Yu; Takemura, Taichiro; Ung, Trang Thi Hong; Le, Thanh Thi; Hirayama, Kazuhiro; Hasebe, Futoshi; Nguyen, Hang Le Khanh; Hoang, Phuong Vu Mai; Nguyen, Cam Nhat; Khong, Tuan Minh; Le, Mai Thi Quynh; Hoang, Ha Thi Thu; Ohnishi, Makoto

doi:10.1016/j.actatropica.2019.02.008

Cited by 14 publications

(12 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although the presence of ST50 L. interrogans has never been reported in Thailand, this Leptospira genotype was found to be a predominant clone circulating in rodents and other small mammal populations in several areas of Malaysia, particularly in the residential areas where suspected cases of human leptospirosis had been frequently reported [16,17]. In recent studies, L. interrogans serogroup Bataviae has been isolated from kidneys of Rattus norvegicus captured in Hanoi [18] and Hai Phong [19], Vietnam. The multilocus variable-number of tandem repeat analysis (MLVA) showed that the Bataviae serogroup isolates were identical to that of the strains causing severe leptospirosis in the 2009 outbreak in the Philippines [18].…”

Section: Discussionmentioning

confidence: 99%

“…In recent studies, L. interrogans serogroup Bataviae has been isolated from kidneys of Rattus norvegicus captured in Hanoi [18] and Hai Phong [19], Vietnam. The multilocus variable-number of tandem repeat analysis (MLVA) showed that the Bataviae serogroup isolates were identical to that of the strains causing severe leptospirosis in the 2009 outbreak in the Philippines [18]. On the other hand, the molecular epidemiological survey of leptospirosis in Laos and Cambodia demonstrated that rodents captured in the urban and rural areas of both countries were reservoir for pathogenic Leptospira especially L. interrogans [20,21].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genotyping of the causative Leptospira in symptomatic dogs in Thailand

Paungpin

Chaiwattanarungruengpaisan

Mongkolphan

et al. 2020

Korean J Vet Res

View full text Add to dashboard Cite

We investigated the genotypes of Leptospira spp. detected in symptomatic dogs in Thailand. During April to December 2012, 6 out of 41 client-owned dogs were diagnosed with leptospirosis based on polymerase chain reaction tests. All of the infected dogs showed clinical symptoms related to leptospirosis. Direct genotyping of the causative agent of the canine leptospirosis was conducted from the archival DNA samples extracted from urine or blood of those 6 infected dogs. Sequencing of the partial 16S rRNA and lipL32 genes from all samples identified Leptospira (L.) interrogans as the infecting species. Multilocus sequence typing tests were successful for 2 out of 6 samples. The sequence type (ST) was identified as ST50 for both samples where the profile corresponded to L. interrogans species and Bataviae serogroup. The presence of this genotype of Leptospira has never been reported in Thailand. Thus, our findings showed the existence of ST50 L. interrogans serogroup Bataviae and the ability to cause leptospirosis in dogs in Thailand.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Genotyping of the causative Leptospira in symptomatic dogs in Thailand

Paungpin

Chaiwattanarungruengpaisan

Mongkolphan

et al. 2020

Korean J Vet Res

View full text Add to dashboard Cite

show abstract

“…It was reported that 8% of the 266 rodents were seropositive, primarily to the serovars Australis and Grippotyphosa in southern Germany (19). It was reported that 27.1% of R. norvegicus were seropositive, and 70% of the positive serum samples reacted with serogroup Bataviae in northern Vietnam, Hanoi, and Bac Giang (20). It was reported that 68.1% of the 142 captured R. norvegicus were seropositive by MAT titer of ≥1:100 in Brazil (17).…”

Section: Discussionmentioning

confidence: 99%

Serological and Molecular Characteristics of Pathogenic Leptospira circulating in rat populations in Fujian Province, China, 2018-2020

Qiu

Liu

et al. 2022

Preprint

View full text Add to dashboard Cite

Background Leptospirosis is a significant emerging infectious disease worldwide. Rodents are considered to be the most critical hosts for Leptospira spp. Fujian Province is a region highly endemic for leptospirosis in China. However, the genetic diversity of leptospires circulating among rodents in Fujian is limited. Results The carrier status of Leptospira spp. in rats was investigated by culturing and serological detection in Fujian during 2018-2020. A total of 710 rodents, including 11 species, were trapped, with R. rattoides being the dominant species (50.56%). Fourteen pathogenic Leptospira strains were obtained. Two species including L. interrogans and L. borgpetersenii were identified using 16S rRNA gene. Four serogroups were identified by the microscopic agglutination test (MAT), including 7 Javanica, 4 Icterohaemorrhagiae, 2 Bataviae and 1 Pyrogenes. Six STs were identified by MLST, including 7 ST143, 2 ST1, 2 ST17, 1 ST96, 1 ST332 and 1 ST333. L. borgpetersenii serogroup Javanica belonging to ST143 was the dominant type (50.00%). A total of 387 rat serum samples were tested by MAT. Sera were considered positive for seroreactivity at a titer ≥1:20 against at least one serovar. A total of 138 (35.66%) sera samples tested positive, and seven serogroups were identified, with Javanica being the dominant serogroup (81.16%), the similar serogroup isolated from rodents. This study demonstrates a high prevalence of leptospirosis in rats and public health education among high-risk workers is highly recommended. Conclusions This study confirmed R. rattoides were the most abundantly rats, and L. borgpetersenii serogroup Javanica ST143, L. interrogans serogroup Icterohaemorrhagiae ST1 and ST17 were widely distributed among rodents in Fujian during 2018-2020. Despite the low number of isolates obtained from rodents, this study suggests that continuous epidemiological surveillance on the etiological characteristics of pathogenic Leptospira in wild animal reservoirs may help reduce the possible risk of disease transmission.

show abstract

“…Unfortunately, this strain could not be cultured in Korthof's medium in this study. Growth in a particular culture medium varies even in the same Leptospira species (Koizumi et al., 2019). A number of specially prepared media have enabled improvements in the growth of fastidious Leptospira strains (Ellis et al., 1985; Hornsby et al., 2020).…”

Section: Discussionmentioning

confidence: 99%

“…Unfortunately, this strain could not be cultured in Korthof's medium in this study. Growth in a particular culture medium varies even in the same Leptospira species (Koizumi et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Rat trade and leptospirosis: Molecular epidemiology of Leptospira species in rats exported from Cambodia to Vietnam

Koizumi

Morita

Pheng

et al. 2021

Transbounding Emerging Dis

Self Cite

View full text Add to dashboard Cite

Rats are an important maintenance host of Leptospira spp., the causative agents of leptospirosis, a zoonotic disease with worldwide distribution. Rats are traded as food in the Mekong Delta, where Cambodia exports tons of rats to Vietnam. Handling wild rats is a potential health risk, but the information on Leptospira spp. carried by rats traded in the region remains limited. In this study, we investigated the carriage of Leptospira spp. in rats exported from Cambodia to Vietnam using bacterial culture, nested PCR and DNA sequencing. Isolates were then assessed using serological analysis and whole‐genome sequencing (WGS), and Leptospira DNA detected in rat kidney tissues was also analysed by multilocus sequence typing (MLST). Seventy‐two rats (2 Bandicota indica, 57 Rattus argentiventer, 11 R. losea, 1 R. norvegicus and 1 R. rattus) were subjected to bacterial culture, and three L. borgpetersenii serogroup Javanica isolates were obtained from R. argentiventer (5.3%). WGS revealed that although Cambodian isolates were genetically related to L. borgpetersenii serogroup Javanica strains widely distributed in East and South‐East Asian countries, they formed a different cluster from other strains. In addition to the three L. borgpetersenii sequences, the flaB sequence of L. interrogans was detected in 18 R. argentiventer and 7 R. losea kidney tissue samples (38.9%) using nested PCR followed by DNA sequencing. The L. interrogans flaB‐positive samples were further analysed by MLST, revealing that seven housekeeping genes (glmU, pntA, sucA, tpiA, pfkB, mreA and caiB) contained novel sequences with distinct lineages from other sequence types. This study revealed a high prevalence of Leptospira spp. among rats exported from Cambodia to Vietnam, indicating a potential risk to people engaging in rat trade and demonstrating that a fastidious L. interrogans strain circulates among Cambodian rats.

show abstract

Molecular epidemiology of Leptospira interrogans in Rattus norvegicus in Hanoi, Vietnam

Cited by 14 publications

References 33 publications

Genotyping of the causative Leptospira in symptomatic dogs in Thailand

Genotyping of the causative Leptospira in symptomatic dogs in Thailand

Serological and Molecular Characteristics of Pathogenic Leptospira circulating in rat populations in Fujian Province, China, 2018-2020

Rat trade and leptospirosis: Molecular epidemiology of Leptospira species in rats exported from Cambodia to Vietnam

Contact Info

Product

Resources

About