Molecular epidemiology of Leptospira spp. among wild mammals and a dog in Amami Oshima Island, Japan

Shinya, So; Muraoka, Yukinori; Negishi, Daigo; Koizumi, Nobuo

doi:10.1371/journal.pone.0249987

Cited by 7 publications

(5 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…For example, a combination of sequence typing and serotyping was used to identify black rats as the likely source of infection of a dog in Japan with L. interrogans serogroup Australis. 42…”

Section: Because Immunity Induced By Vaccination With Current Leptospiramentioning

confidence: 99%

“…Because the pattern of seroreactivity identified by microscopic agglutination test (MAT) serology does not reliably predict the infecting serogroup, 41 and many STs belong to a serogroup, accurate identification of potential sources of infection requires sequence typing. For example, a combination of sequence typing and serotyping was used to identify black rats as the likely source of infection of a dog in Japan with L. interrogans serogroup Australis 42 …”

Section: Introduction and Epidemiologymentioning

confidence: 99%

See 1 more Smart Citation

Updated ACVIM consensus statement on leptospirosis in dogs

Sykes,

Francey,

Schuller

et al. 2023

Veterinary Internal Medicne

View full text Add to dashboard Cite

Since publication of the last consensus statement on leptospirosis in dogs, there has been revision of leptospiral taxonomy and advancements in typing methods, widespread use of new diagnostic tests and vaccines, and improved understanding of the epidemiology and pathophysiology of the disease. Leptospirosis continues to be prevalent in dogs, including in small breed dogs from urban areas, puppies as young as 11 weeks of age, geriatric dogs, dogs in rural areas, and dogs that have been inadequately vaccinated for leptospirosis (including dogs vaccinated with 2‐serovar Leptospira vaccines in some regions). In 2021, the American College of Veterinary Internal Medicine (ACVIM) Board of Regents voted to approve the topic for a revised Consensus Statement. After identification of core panelists, a multidisciplinary group of 6 experts from the fields of veterinary medicine, human medicine, and public health was assembled to vote on the recommendations using the Delphi method. A draft was presented at the 2023 ACVIM Forum, and a written draft posted on the ACVIM website for comment by the membership before submission to the editors of the Journal of Veterinary Internal Medicine. This revised document provides guidance for veterinary practitioners on disease in dogs as well as cats. The level of agreement among the 12 voting members (including core panelists) is provided in association with each recommendation. A denominator lower than 12 reflects abstention of ≥1 panelists either because they considered the recommendation to be outside their scope of expertise or because there was a perceived conflict of interest.

show abstract

Section: Because Immunity Induced By Vaccination With Current Leptospiramentioning

confidence: 99%

Section: Introduction and Epidemiologymentioning

confidence: 99%

Updated ACVIM consensus statement on leptospirosis in dogs

Sykes,

Francey,

Schuller

et al. 2023

Veterinary Internal Medicne

View full text Add to dashboard Cite

show abstract

“…In Algeria, L. interrogans was implicated in many cases of human leptospirosis between 2005 and 2008 [21,22] and was identified in 5 dogs in 2017 in northern Algeria [23]. L. borpetersenii was found in a single individual in our survey; this species was also associatedwith rats species; Rattus Norvegicus in South Africa [59] and Madagascar [14] and Rattus Rattus in Sardinia [49] and Japan [60]. The low rate of carriage of L. borpetersenii in rodents observed in the present study can be explained by the fact that L. borpetersenii colonizesfewer rat kidneys [61] when compared to L. interrogans known to persist for several months in kidneys [44].…”

Section: Discussionmentioning

confidence: 55%

First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas

et al. 2022

View full text Add to dashboard Cite

Leptospirosis is an anthropozoonosis disease of worldwide distribution caused by mobile spirochetes of the genus Leptospira and rodents, mainly rats, are described as its primary reservoir. In Algeria, there is limited data about the prevalence of Leptospira spp. in humans and animals, as well as Leptospira carriage in wild rodents. The study aimed to highlight the importance of rodents as a reservoir of Leptospira bacterium in Blida city in Algeria by detecting and identifying circulating Leptospira species in the rodent population. A total of 101 rodents, 95 Rattus Norvegicus, 5 Rattus Rattus, and 1 Mus Musculus were captured and tested for pathogenic Leptospira spp. byreal-time PCR targeting the Leptospira 16S rRNA (rrs) gene, revealing a total prevalence of 40.6%, 95% IC [30.9–50.8%]. Positive samples were subjected to species-specific real-time PCR assays targeting L. interrogans, L. noguchii, L. borgpetersenii, and L. kirschneri for species identification. However, positive samples for which Leptospira-species could not be determined were subjected to conventional PCR targeting the partial 16S rRNA (rrs) gene, and amplified DNA was subjected to sequencing. Leptospira spp. was detected in 36 kidney, 16 urine, and three lung specimens. L. interrogans was identified in 39 rodents and L. borpetersenii in one rodent; however, one rodent with renal carriage could not be typed due to poor DNA quality. This study provides the first description of pathogenic Leptospira spp. in wild rodents in Algeria. These findings suggest a high potential risk of leptospirosis transmission from rodents to humans and animals in Algeria and therefore imply the adoption of prophylactic measures. In addition, further studies, including different animals and rodent species, should be conducted to clarify the epidemiology of this disease in Algeria.

show abstract

“…Contaminated water or the environment, transmission through rodents' urine, and the presence of livestock as reservoirs are all critical sources of disease transmission. Leptospires are found in mammals [5] such as cattle, buffalo, horses, goats, dogs, cats, [6] and other rodents [7]. Rodents were first discovered as reservoirs and natural hosts capable of sustaining the presence of Leptospira without exhibiting symptoms.…”

Section: Introductionmentioning

confidence: 99%

Infestation of Leptospira in Rodents at the Perimeter Area in “Pelabuhan Ratu” Port, Sukabumi District

Santi¹,

Astuti²,

Yuliasih³

et al. 2023

Proceedings of the 1st International Conference for Health Research – BRIN (ICHR 2022)

View full text Add to dashboard Cite

Leptospirosis is a neglected zoonotic disease with global health implications. A significant impact of Leptospirosis happens in tropical regions, including Indonesia. Rodents play an essential role in the spread of Leptospirosis due to their rapid population growth and proximity to humans. This study aims to determine the rodent population density and the presence of leptospira bacterial infestations in the proximity of the "Pelabuhan Ratu" Port, Sukabumi district. This study's survey was conducted monthly for six months (January, February, March, May, June, and August) in 2021 and for three months (January, March, and April) in 2022. The study's survey was held for 15-16 h; a single trap was used to catch rodents. One hundred traps are placed on the port's perimeter inside and outside the building. Leptospira bacteria were detected using PCR in rodents captured between January and March 2022. According to the survey results, 120 rodents were captured, and the sex proportions of rodents collected in the survey were nearly equal. Rattus norvegicus, Rattus tanezumi, and Bandicota indica were all captured. The average population of dominant rodents in the 2021 survey was R. tanezumi (4.3), while R. norvegicus (17.3) was mainly found in the 2022 survey. The average success rate of traps in 2021-2022 was 7.83 and 24.3, respectively. Rodents were found to have Leptospira bacteria (29.09%). The density of rodents in the proximity of the "Pelabuhan Ratu" port is considered high (> 1). Leptospira is only found in R. norvegicus caught at the fish market in this study site. This information is critical for early detection in the effort to raise public awareness of the occurrence of Leptospirosis transmission. It is necessary to improve communication, information, and education to traders and visitors in maintaining personal and environmental hygiene and cross-sectoral collaboration to improve environmental sanitation, particularly in public spaces.

show abstract

Molecular epidemiology of Leptospira spp. among wild mammals and a dog in Amami Oshima Island, Japan

Cited by 7 publications

References 37 publications

Updated ACVIM consensus statement on leptospirosis in dogs

Updated ACVIM consensus statement on leptospirosis in dogs

First Molecular Detection of Pathogens Leptospira in Common Rodent Captured in North Algeria Urban Areas

Infestation of Leptospira in Rodents at the Perimeter Area in “Pelabuhan Ratu” Port, Sukabumi District

Contact Info

Product

Resources

About