Molecular Epidemiology of Leptospirosis in Northern Iran by Nested Polymerase Chain Reaction/Restriction Fragment Length Polymorphism and Sequencing Methods

Zakeri, Sedigheh; Sepahian, Neda; Afsharpad, Mandana; Esfandiari, Behzad; Ziapour, Seyyed Payman; Djadid, Navid D.

doi:10.4269/ajtmh.2010.09-0721

Cited by 36 publications

(39 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The current available whole-cell vaccines cannot provide cross-protection against infection with more than 250 different Leptospira serovars known to exist and may lead to incomplete, short-term immunity and serious side effects. Despite vaccination, the disease still exists in some parts of the country [6][7][8]. The design and construction of an efficient recombinant vaccine for leptospirosis control is very important [9,10].…”

Section: Introductionmentioning

confidence: 99%

Molecular identification of the ompL1 gene within Leptospira interrogans standard serovars

Dezhbord

Esmaelizad

Khaki

et al. 2014

J Infect Dev Ctries

View full text Add to dashboard Cite

Introduction: Leptospirosis, caused by infection with pathogenic Leptospira species, is one of the most prevalent zoonotic diseases in the world. Current leptospiral vaccines are mainly multivalent dead whole-cell mixtures made of several local dominant serovars. Therefore, design and construction of an efficient recombinant vaccine for leptospirosis control is very important. OmpL1 is an immunogenic porin protein that could be of special significance in vaccination and serodiagnosis for leptospirosis. Methodology: Three strains belonging to pathogenic L. interrogans were analyzed. The specific primers for proliferation of the ompL1 gene were designed. The amplified gene was cloned. In order to investigate the ompL1 nucleotide sequence and homological analysis of this gene, ompL1 genes cloned from standard vaccinal Leptospira serovars prevalent in Iran were sequenced and cloned. Results: PCR amplification of the ompL1 gene using the designed primers resulted in a 963 bp ompL1 gene product. The PCR based on the ompL1 gene detected all pathogenic reference serovars of Leptospira spp. tested. Based on alignment and phylogenetic analysis, although the ompL1 nucleotide sequence was slightly different within three vaccinal serovars (100%-85% identity), amino acid alignment of the OmpL1 proteins revealed that there would be inconsiderable difference among them. Conclusion: The ompL1 gene of the three isolates was well conserved, differing only by a total of 6 bp and the proteins by 2 amino acids. The cloned gene could be further used for expression and recombinant OmpL1 as an efficient and conserved antigen, and may be a useful vaccine candidate against leptospirosis in our region.

show abstract

Section: Introductionmentioning

confidence: 99%

Molecular identification of the ompL1 gene within Leptospira interrogans standard serovars

Dezhbord

Esmaelizad

Khaki

et al. 2014

J Infect Dev Ctries

View full text Add to dashboard Cite

show abstract

“…In the study of Golsha et al in Golestan, fever, chilling and myalgia (100%) were reported as the most common clinical symptoms of leptospirosis (Golsha et al, 2007). Fever and myalgia were the most common symptoms of leptospirosis in Zakeri et al's research (Zakeri et al, 2010). In the study of Sehgal and Katz, among the various clinical symptoms, fever and headache have been referred as the most common symptoms (Katz et al, 2011;Sehgal, 2006).…”

Section: Discussionmentioning

confidence: 99%

Evaluation of Clinical Symptoms Related to Common Serogroups of Leptospirosis in North of Iran

Javanian¹,

Zavareh²,

Vahedi³

et al. 2017

JMBR

View full text Add to dashboard Cite

Leptospirosis is a common disease between humans and animals. Identifying common serogroups and comparing different clinical symptoms among them can help in finding the clinical pattern associated with pathogen serogroupes of leptospirosis. Therefore, the purpose of this study has been to investigate the common serotypes of the infection and their clinical symptoms in northern Iran. This cross-sectional study was carried out in educational hospitals of Babol University of Medical Sciences during the years 2011-2014. Subjects with clinical findings consistent with leptospirosis were included in the study. According to the standard MAT guidelines, the titre ≥1: 200 was considered positive. Then, the patients identified by serogroup separation, were examined and compared clinical symptoms. Among 6o patients with primary diagnosis of leptospirosis in this study, 35 of them proved to be infected to the disease. The most common serogroups were serjoe (40%) and icterohemorrhagia (31.4%). Autumnalis (22.8%), grippotyphosa (11.4%), canicola (8.6%), and pomona (2.8%) were included the subsequent serogroups. The highest frequency of fever (28%) and gastrointestinal manifestations (36%) were observed in icterohemorrhagia and the highest icterus (30%) was found in serjoe serogroup. In this study, serjoe serogroup with fever and icterus, and then icterohemorrhagia with fever and gastrointestinal symptoms were introduced as the most common serogroups of Leptospirosis. Also the rarest serogroups were canicola and pomona.

show abstract

“…To overcome all these limitations polymerase chain reaction (PCR) have been used, which is considered sensitive and specific for the rapid detection of Leptospira in clinical samples. Evaluation of PCR-based studies for the identification and diagnosis of leptospirosis has been reported elsewhere, in humans and animals [13,28,29,30]. It has been developed for the rapid detection of Leptospira DNA and has the potential to make a dramatic impact in diagnosing leptospirosis [25].…”

Section: Polymerase Chain Reaction (Pcr)mentioning

confidence: 99%

A Review of Laboratory Diagnosis and Treatment of Leptospirosis

Al-orry¹,

Arahou²,

Hassikou³

et al. 2016

Int J Pharm Pharm Sci

View full text Add to dashboard Cite

Leptospirosis is an infectious disease affect human and animal. The diagnosis is challenging due to none specific clinical features, lack of awareness and poor access to health care. Therefore the disease becomes underreported. Early diagnosis is essential because antibiotic treatment is most effective when it is initiated early. However, laboratories tests for early diagnosis still lacking. The disease may be demonstrated by direct or indirect methods. Direct methods are the detection of the organism or its components and indirect methods are the detection of specific antibodies of the organism. This review highlights the major diagnostic techniques in leptospirosis with their advantages and disadvantages as well as the treatment. The search criteria focused on the thesis we obtained and searching in the websites (Google

show abstract

Molecular Epidemiology of Leptospirosis in Northern Iran by Nested Polymerase Chain Reaction/Restriction Fragment Length Polymorphism and Sequencing Methods

Cited by 36 publications

References 16 publications

Molecular identification of the ompL1 gene within Leptospira interrogans standard serovars

Molecular identification of the ompL1 gene within Leptospira interrogans standard serovars

Evaluation of Clinical Symptoms Related to Common Serogroups of Leptospirosis in North of Iran

A Review of Laboratory Diagnosis and Treatment of Leptospirosis

Contact Info

Product

Resources

About