Molecular Epidemiology of Porcine Cytomegalovirus (PCMV) in Sichuan Province, China: 2010–2012

Liu, Xiao; Liao, Shan; Zhu, Ling; Xu, Zhiwen; Zhou, Yuancheng

doi:10.1371/journal.pone.0064648

Cited by 25 publications

(25 citation statements)

References 27 publications

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“…Phylogenetic analysis based on the complete DPOL gene nucleotide sequences demonstrated that all the PCMV isolates share very close relationship, belonging to the same cluster together with HHV-6 and HHV-7 under the genus Roseolovirus, rather than HHV-5 (under the genus Cytomegalovirus). ese data suggested that the PCMV can be classified as a member of the genus Cytomegalovirus under the subfamily Betaherpesvirinae using DPOL gene (Figure 1), which is in accordance with the phylogenetic tree based on the gB gene [25].…”

Section: Dpol Gene Analysissupporting

confidence: 82%

Development of a TaqMan Based Real‐Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen

Chen

et al. 2020

BioMed Research International

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This study described a TaqMan based real-time fluorescent quantitative PCR (qPCR) method to detect porcine cytomegalovirus (PCMV) infection, targeting the conserved region of the DNA polymerase (DPOL) gene. The standard curve showed a linear regression relationship with a coefficient of 0.999 and a slope of y = −3.249x + 38.958 corresponding to the amplification efficiency at 99.8%. The limit of the qPCR method was 51.9 copies/μl. The established qPCR method showed excellent specificity, with no cross-reaction observed with common porcine pathogens. The coefficient of variation for intra-assay and interassay variability ranged up to 1.51% and 2.24%, respectively. PCMV positive signals can be found in semen using this qPCR method, which suggested that we should pay more attention to PCMV contamination in semen in order to eliminate PCMV infection in artificial insemination and xenotransplantation.

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Section: Dpol Gene Analysissupporting

confidence: 82%

Development of a TaqMan Based Real‐Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen

Chen

et al. 2020

BioMed Research International

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“…Similar to the coinfection of humans with immunosuppressive viruses, the infection of animals with immunosuppressive viruses, followed by secondary viral and bacterial infections, can cause serious harm and inhibit disease prevention and treatment. In a previous study in Sichuan Province, China, we observed that the PCMV and PRRSV coinfection rate in pig was 34.7%, while the PCMV/PRRSV/Thermoproteus tenax virus 1/ pseudorabies virus coinfection rate was 14.9% [30].…”

Section: Discussionmentioning

confidence: 80%

“…Although our previous study confirmed that PCMV suppresses the immune function of porcine central immune organs, there has been little research on the molecular mechanism behind PCMV immunosuppression. The miRNA transcriptome of porcine continuous cell lines was previously reported, but there have been no reports on the miRNA transcriptome of PCMV-infected vital organs [22,30,31].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection

Liu

Wei

Liao

et al. 2018

Virol J

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BackgroundPorcine cytomegalovirus (PCMV) is an immunosuppressive virus that mainly inhibits T-lymphocyte and macrophage immune functions; it has significantly damaged the farming industry. Although recent studies have shown that miRNAs play important roles in immune responses, the regulatory mechanisms of miRNAs during immunosuppressive virus infection remain unclear.MethodsIn this study, porcine small-RNA transcriptomes of PCMV-infected and uninfected vital organs were first characterised by high-throughput sequencing. miRDeep2 software was used to predict novel pig-encoded miRNAs. To verify the accuracy of the high-throughput sequencing results, stem-loop qRT-PCR was performed on 12 significantly DE miRNAs.The physical and functional interactions between the immune-related target genes of the DE miRNAs in PCMV-infected organs were analysed using the STRING database.ResultsIn total, 306 annotated and 295 novel miRNAs were identified from PCMV-infected and uninfected porcine organs, respectively, through alignment with known Sus scrofa pre-miRNAs. Overall, 92, 107, 95, 77 and 111 miRNAs were significantly differentially expressed in lung, liver, spleen, kidney and thymus after PCMV infection, respectively.According to Gene Ontology enrichment analysis, target genes of the differentially expressed miRNAs associated with immune system processes, regulation of biological processes and metabolic processes were enriched in every sample. Integrated expression analysis of the differentially expressed miRNAs and their target mRNAs in PCMV-infected thymus showed that the significant differential expression of specific miRNAs under the pressure of PCMV infection in central immune organs interfered with the expression of genes involved in important immune-related signalling pathways, thus promoting the viral infection.ConclusionsThis is the first comprehensive analysis of the responses of host small-RNA transcriptomes to PCMV infection in vital porcine organs. It provides new insights into the regulatory mechanisms of miRNAs during infection by immunosuppressive viruses.Electronic supplementary materialThe online version of this article (10.1186/s12985-018-0922-x) contains supplementary material, which is available to authorized users.

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“…Based on the results of clinical testing, some PCMV/PRV-infected animals were co-infected with porcine reproductive and respiratory syndrome virus (PRRSV), transfusion transmitted virus (TTV1), and pseudorabies virus (PRV). In addition, Streptococcus, Haemophilus parasuis, Actinobacillus, and Pasteurella suis infections often occur following PCMV/PRV infection, making prevention and treatment more difficult [177]. A transcriptome analysis of PCMV/PRV -infected thymuses showed an up-and downregulation of immune-regulatory genes [178].…”

Section: Pathogenicitymentioning

confidence: 99%

Comparative Analysis of Roseoloviruses in Humans, Pigs, Mice, and Other Species

Denner

Bigley

Phan

et al. 2019

Viruses

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Viruses of the genus Roseolovirus belong to the subfamily Betaherpesvirinae, family Herpesviridae. Roseoloviruses have been studied in humans, mice and pigs, but they are likely also present in other species. This is the first comparative analysis of roseoloviruses in humans and animals. The human roseoloviruses human herpesvirus 6A (HHV-6A), 6B (HHV-6B), and 7 (HHV-7) are relatively well characterized. In contrast, little is known about the murine roseolovirus (MRV), also known as murine thymic virus (MTV) or murine thymic lymphotrophic virus (MTLV), and the porcine roseolovirus (PRV), initially incorrectly named porcine cytomegalovirus (PCMV). Human roseoloviruses have gained attention because they can cause severe diseases including encephalitis in immunocompromised transplant and AIDS patients and febrile seizures in infants. They have been linked to a number of neurological diseases in the immunocompetent including multiple sclerosis (MS) and Alzheimer's. However, to prove the causality in the latter disease associations is challenging due to the high prevalence of these viruses in the human population. PCMV/PRV has attracted attention because it may be transmitted and pose a risk in xenotransplantation, e.g., the transplantation of pig organs into humans. Most importantly, all roseoloviruses are immunosuppressive, the humoral and cellular immune responses against these viruses are not well studied and vaccines as well as effective antivirals are not available. that cause various clinical disease and are classified into the alpha-, beta-and gammaherpesvirinae. Roseoloviruses belong to the Betaherpesvirinae: roseoloviruses that infect humans, swine, and mice and have a high prevalence in their respective hosts and will be addressed in this review.

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Molecular Epidemiology of Porcine Cytomegalovirus (PCMV) in Sichuan Province, China: 2010–2012

Cited by 25 publications

References 27 publications

Development of a TaqMan Based Real‐Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen

Development of a TaqMan Based Real‐Time Fluorescent Quantitative PCR Assay for Detection of Porcine Cytomegalovirus in Semen

MicroRNA transcriptome analysis of porcine vital organ responses to immunosuppressive porcine cytomegalovirus infection

Comparative Analysis of Roseoloviruses in Humans, Pigs, Mice, and Other Species

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