Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia

Tamayo, Marcela Henao; Koblavi, S.; Grimont, Francine; Castañeda, E.; Grimont, P. A. D.

doi:10.1099/00222615-46-7-611

Cited by 24 publications

(20 citation statements)

References 20 publications

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“…These ribotypes were identical to patterns previously reported in BglI ribotype schemes of V. cholerae O1 strains, designed B5 or CDC ribotype 5 and CDC ribotype 6a or ribotype B21a, respectively (21,27,35). The presence of two strains in Mexico, each corresponding to one of these two ribotypes, was reported by other authors who studied the strains isolated in that country during the 1991 to 1995 epidemic period.…”

Section: Discussionsupporting

confidence: 71%

“…A second and distinct epidemic strain, V. cholerae O1 biotype El Tor serotype Ogawa, was later associated with the Mexican epidemic. This strain, reported by Popovic et al (27) to be ribotype 6a, identical to ribotype B21a described by Tamayo et al (35), belonged to electrophoretic type 3 (37) and was initially isolated in 1991 in Latin America from cholera cases occurring in Mexico and Central America. It may have been responsible for a second epidemic wave (14).…”

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confidence: 99%

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Molecular Analyses of Vibrio cholerae O1 Clinical Strains, Including New Nontoxigenic Variants Isolated in Mexico during the Cholera Epidemic Years between 1991 and 2000

Lizárraga-Partida

Quilici

2009

J Clin Microbiol

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We studied the evolution of Vibrio cholerae O1 during the 1991 to 2000 cholera epidemic in Mexico by biochemical, serological, and molecular characterization of strains collected during this period. Strains were divided into toxigenic and nontoxigenic groups according to the presence or absence of genes encoding cholera toxin. As previously reported, we characterized two populations among toxigenic strains, which were present from the first year of the epidemic. BglI rRNA analysis revealed that these strains had ribotype profiles, denoted M5 and M6 in our study, that were identical to those previously designated Koblavi B5 or Popovic 5 and Popovic 6a or Tamayo B21a, respectively. Ribotype M5 was isolated between 1991 and 1993. This ribotype had a low level of genetic variation as detected by pulsed-field gel electrophoresis (PFGE). Ribotype M6 persisted from 1991 to 2000. However, PFGE profiles suggested that two epidemiologically unrelated strains coexisted within this single ribotype from 1995 until the end of the epidemic. We identified three new BglI ribotypes, Mx1, Mx2, and Mx3, from nontoxigenic V. cholerae O1 strains isolated between 1998 and 2000; one of them grouped strains positive for the toxin-coregulated pilus island. They differed from nontoxigenic clones isolated in Latin America and on the U.S. Gulf Coast and are probably autochthonous Mexican V. cholerae O1 variants. Most of these new variants were isolated from states surrounding the Gulf of Mexico, where the highest incidence of cholera in the country was recorded. Thus, the Mexican Gulf Coast, like the U.S. Gulf Coast, may act as an environmental reservoir of V. cholerae O1.

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Section: Discussionsupporting

confidence: 71%

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confidence: 99%

Molecular Analyses of Vibrio cholerae O1 Clinical Strains, Including New Nontoxigenic Variants Isolated in Mexico during the Cholera Epidemic Years between 1991 and 2000

Lizárraga-Partida

Quilici

2009

J Clin Microbiol

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“…The amplicons were electrophoresed through agarose 0.8% gel (Appligene, Illkirch, France) and then stained with ethidium bromide. A negative control (reaction mixture without template) and a toxin positive control (V. cholerae O1 strain O395) were included in each run [10].…”

Section: Gene Detection By Pcrmentioning

confidence: 99%

Molecular epidemiological study of Vibrio cholerae isolates from infected patients in Teheran, Iran

Pourshafie¹,

Grimont

Saifi³

et al. 2000

Journal of Medical Microbiology

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A total of 110 clinical isolates of Vibrio cholerae O1 biotype El Tor serotype Ogawa isolated in a recent outbreak from different districts of Teheran, Iran, was subjected to 99 carbon source utilisation tests, ribotyping and toxinogenotyping. PCR showed that the genes encoding cholera toxin (ctxA), toxin co-regulated pilus (tcp), accessory cholera enterotoxin (ace) and zonula occludens toxin (zot) were present in 100%, 100%, 97.3% and 99.1% of the isolates, respectively. Restriction fragment length polymorphism (RFLP) study of the BglI-digested DNA probed with ®ve oligonucleotides targeting the conserved regions of 16S and 23S rRNA genes revealed a similar ribotype pattern for 109 isolates. All but one isolate showed ribotype pattern B21a, containing seven bands with molecular sizes ranging from 11 to 3.9 kb. The toxin gene restriction pattern (toxinogenotype) showed that the isolates carried either three or two copies of the toxin genes (ace, zot, ctx) which were recognised as TB41a and TB69 patterns, respectively. Overall, the ribotyping data showed that, despite biochemical differences in 12 of the 99 carbon sources, most of the isolates studied belonged to a single clone.

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“…Detection of genes encoding cholera toxin subunit A (ctxA), zonula occludens toxin (zot), accessory cholera enterotoxin (ace), and toxin-coregulated pilus for intestinal colonization (tcpA) was performed by PCR as described previously (10,16). A negative control (reaction mixture without template) and a toxin-positive control (V. cholerae O1 strain O395) were also included.…”

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confidence: 99%

Clonal Relationship among Vibrio cholerae O1 El Tor Strains Causing the Largest Cholera Epidemic in Kenya in the Late 1990s

Scrascia

Maimone

Mohamud³

et al. 2006

J Clin Microbiol

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Eighty Vibrio cholerae O1 strains selected to represent the 1998-to-1999 history of the largest cholera epidemic in Kenya were characterized by ribotyping, antimicrobial susceptibility, and random amplified polymorphic DNA patterns. Except for 19 strains from 4 local outbreaks in North Eastern Province along the Somalia border, the other 61 strains from 25 outbreaks occurring in districts scattered around the country were all ribotype B27 and resistant to chloramphenicol, spectinomycin, streptomycin, sulfamethoxazole, and trimethoprim. The 61 strains showed similar and specific amplified DNA patterns. These findings indicate that the predominant strains that caused the Kenyan epidemic had a clonal origin and suggest that ribotype B27 strains, which first appeared in West Africa in 1994, have had a rapid spread to eastern Africa.

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Molecular epidemiology of Vibrio cholerae O1 isolates from Colombia

Cited by 24 publications

References 20 publications

Molecular Analyses of Vibrio cholerae O1 Clinical Strains, Including New Nontoxigenic Variants Isolated in Mexico during the Cholera Epidemic Years between 1991 and 2000

Molecular Analyses of Vibrio cholerae O1 Clinical Strains, Including New Nontoxigenic Variants Isolated in Mexico during the Cholera Epidemic Years between 1991 and 2000

Molecular epidemiological study of Vibrio cholerae isolates from infected patients in Teheran, Iran

Clonal Relationship among Vibrio cholerae O1 El Tor Strains Causing the Largest Cholera Epidemic in Kenya in the Late 1990s

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