Molecular evidence for hybrid origin of Melastoma intermedium

Dai, Seping; Wu, Wei; Zhang, Rongshu; Liu, Ting; Chen, Yunyun; Shi, Suhua; Zhou, Renchao

doi:10.1016/j.bse.2011.12.010

Cited by 20 publications

(32 citation statements)

References 11 publications

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“…[11,15,27,28], these methods, however, either fail to provide convincing conclusions or are time and labor consuming, especially when the sample size is large. Recently, single or low-copy nuclear genes have proven successful in identifying hybrids in plants [14,29-35]. In this study, we aimed to test the hypothesis of natural hybridization between A. aureum and A. speciosum using the sequences of three low-copy nuclear genes.…”

Section: Introductionmentioning

confidence: 99%

Molecular evidence for natural hybridization in the mangrove fern genus Acrostichum

Zhang

Liu

et al. 2013

BMC Plant Biol

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BackgroundNatural hybridization is prevalent in ferns, and plays an important role in fern evolution and speciation. In the Indo West-Pacific region, the mangrove fern genus Acrostichum consists of two largely sympatric species, A. aureum and A. speciosum. Although there has been no report of interspecific hybridization before, we found some individuals morphologically intermediate between them in Guangdong and Hainan, China, for the first time, which were suspected to be hybrids. In this study, we aimed to test the hypothesis of natural hybridization between A. aureum and A. speciosum in Guangdong and Hainan using three low-copy nuclear genes. A chloroplast intergenic spacer was used to infer the hybridization direction once the hybrid status was confirmed. In addition, we examined spore shapes and germination for these taxa.ResultsBoth A. aureum and A. speciosum showed a low level of polymorphism at all three nuclear genes; however, they were well separated at these loci. At both locations, each individual of the putative hybrid showed additivity in chromatograms at all sites where the two species showed fixed differences. Haplotype analysis at all three nuclear genes indicated that each individual of the putative hybrid possessed two haplotypes, matching with those of A. aureum and A. speciosum, respectively. Sequencing of the chloroplast trnV-trnM regions showed that A. aureum differed from A. speciosum by eleven nucleotide substitutions and three indels (insertions/deletions), and all sampled individuals of the putative hybrid had the identical sequences with A. speciosum. Compared with A. aureum and A. speciosum, the putative hybrid had much reduced spore germination rate.ConclusionsSequence data of the three nuclear genes provide compelling evidence for natural hybridization between A. aureum and A. speciosum, and all the hybrid individuals are likely F1s. The hybridization is unidirectional and A. speciosum is the maternal parent of the hybrid based on the assumption of maternal inheritance of chloroplast DNA. Human disturbance on mangrove habitats may facilitate the establishment of hybrids of Acrostichum.

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Section: Introductionmentioning

confidence: 99%

Molecular evidence for natural hybridization in the mangrove fern genus Acrostichum

Zhang

Liu

et al. 2013

BMC Plant Biol

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“…Recent studies have also found widespread hybridization among the different species (e.g. Dai et al 2012;Liu et al 2014;Wong 2015), further complicating species delineation within the genus. Here, we report the complete chloroplast genome sequence of M. candidum, commonly found in southern China, as a resource for future studies on the taxonomy of Melastoma.…”

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confidence: 99%

The complete chloroplast genome sequence of Melastoma candidum (Melastomataceae)

Cai

et al. 2017

Mitochondrial DNA Part B

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The plant genus Melastoma is comprised of members estimated to have formed through recent species radiation. Natural hybridization among member species further complicates taxonomy within the genus. Herein, we report the complete chloroplast genome of M. candidum , assembled from partial data obtained from a parallel whole-genome Illumina paired-end sequencing effort on the species. The chloroplast genome was 156,682 bp in length, with a large single-copy (LSC) region of 86,084 bp, a small single-copy (SSC) region of 17,094 bp, separated by two inverted repeat (IR) regions of 26,752 bp each. It was predicted to contain a total of 129 genes, with an overall GC content of 37.17%. Phylogenetic analysis placed M. candidum in the same clade as species within the Melastomeae tribe of Melastomataceae.

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“…Borneo is the third biggest island in the world and the most in-depth research in Melastoma has been conducted here (Meyer, 2001;Wong, 2016 coexist and the putative hybrid shows morphological intermediacy between the two species ( Figure 1). Previous studies showed that sequences of low-copy nuclear genes were very useful for hybrid identification in Melastoma (Dai et al, 2012;Zhou et al, 2017;Zou et al, 2017), and we used this approach in this study. Melastoama beccarianum and M. malabathricum are covered by penicillate emergences with long spreading bristles and short imbricate or appressed lanceolate scales on their hypanthiums, respectively, while the putative hybrid is covered by long branched appressed scales on its hypanthiums.…”

Section: Introductionmentioning

confidence: 99%

“…Melastoma has undergone rapid species radiation in the past 1 million years (Renner & Meyer, 2001). Hybridization is frequently reported amongst species pairs in this genus (Dai et al, 2012;Zhou et al, 2017;Zou et al, 2017). However, all these reported cases of hybridization in Melastoma were confined to species in China.…”

Section: Introductionmentioning

confidence: 99%

“…As hybrid identification based on morphology alone is not always reliable, we aimed to test the hypothesis of natural hybridization between M. malabathricum and M. beccarianum by molecular means. Previous studies showed that sequences of low-copy nuclear genes were very useful for hybrid identification in Melastoma (Dai et al, 2012;Zhou et al, 2017;Zou et al, 2017), and we used this approach in this study.…”

Section: Introductionmentioning

confidence: 99%

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Molecular identification of natural hybridization betweenMelastoma malabathricumandMelastoama beccarianumin Sarawak, Malaysia

Zou

Tan³

et al. 2019

Ecology and Evolution

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Hybridization is very common in flowering plants and it plays a significant role in plant evolution and adaptation. Melastoma L. (Melastomataceae) comprises about 80–90 species in tropical Asia and Oceania, among which 41 species occur in Borneo. Natural hybridization is frequently reported in Melastoma in China, but so far there have been no confirmed cases of hybridization in Southeast Asia (including Borneo), where most species occur. Here, we identified a case of natural hybridization between Melastoma malabathricum L. and Melastoma beccarianum Cogn. in Sarawak, Malaysia, by using sequence data of three nuclear genes and one chloroplast intergenic spacer. Melastoma malabathricum is the most widespread species of this genus, occurring in almost the whole range of this genus, while M. beccarianum is a local species endemic to northern Borneo. Our results showed that natural hybridization and introgression occur between M. malabathricum and M. beccarianum, and the introgression was asymmetrical, mainly from M. malabathricum to M. beccarianum. As adaptive traits can be transferred by introgression, our study suggests that natural hybridization should be a significant mechanism for the evolution and adaptation of Melastoma in Southeast Asia. However, introgression from the common species M. malabathricum to the relatively rare species M. beccarianum may cause the decline of M. beccarianum, incurring conservation concern. With a large number of species of Melastoma and almost year‐around flowering in Southeast Asia, more cases of natural hybridization are expected to be found and identified in near future.

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Molecular evidence for hybrid origin of Melastoma intermedium

Cited by 20 publications

References 11 publications

Molecular evidence for natural hybridization in the mangrove fern genus Acrostichum

Molecular evidence for natural hybridization in the mangrove fern genus Acrostichum

The complete chloroplast genome sequence of Melastoma candidum (Melastomataceae)

Molecular identification of natural hybridization betweenMelastoma malabathricumandMelastoama beccarianumin Sarawak, Malaysia

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