Molecular Evidence for<i>Anaplasma phagocytophilum</i>in Israel

Keysary, Avi; Massung, Robert F.; Inbar, Moshe; Wallach, Arian D.; Shanas, Uri; Mumcuoglu, Kosta Y.; Waner, Trevor

doi:10.3201/eid1309.070455

Cited by 26 publications

(21 citation statements)

References 15 publications

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“…3 Anaplasma phagocytophilum DNA has been detected from ticks in Israel, Japan, and China. [4][5][6] The HGA was first reported in China in 2008 and the first HGA outbreak in China was noteworthy because it caused nosocomial infection. 7 Because HGA only emerged recently in China, the pathogen of HGA has not yet been isolated and the epidemic data is incomplete.…”

Section: Introductionmentioning

confidence: 99%

Seroprevalence of Human Granulocytotropic Anaplasmosis in Central and Southeastern China

Zhang

Hai

Li³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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Human granulocytic anaplasmosis (HGA) is an emerging tick-borne infectious disease. To determine the prevalence of HGA in central and southeastern China, a total of 323 human sera were collected from individuals at high risk for exposure to ticks and animals. The IgG antibody against the etiologic agent of HGA, Anaplasma phagocytophilum was detected with indirect immunofluorescence assay. The results showed that 20% of the tested individuals (64/323) were positive to A. phagocytophilum and that the incidence was higher in male (22%) than female (16%). We concluded that A. phagocytophilum infection was prevalent in central and southeastern China.

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Section: Introductionmentioning

confidence: 99%

Seroprevalence of Human Granulocytotropic Anaplasmosis in Central and Southeastern China

Zhang

Hai

Li³

et al. 2009

The American Journal of Tropical Medicine and Hygiene

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“…phagocytophilum is the causative agent of granulocytic anaplasmosis in various mammals, including humans (Keysary et al 2007). Growing attention has been given worldwide to this pathogenic agent since the first report of human granulocytic anaplasmosis was recorded in the USA (Sheng-Min et al 1994).…”

Section: Discussionmentioning

confidence: 99%

“…Ixodes ricinus is considered to be the major vector throughout the Europe (Parola and Raoult 2001). Nevertheless, ticks of genera Rhipicephalus, Hyalomma, and Haemaphysalis are also involved in transmission in the East Mediterranean area (Keysary et al 2007;Psaroulaki et al 2008).…”

Section: Introductionmentioning

confidence: 99%

Stray dogs of northern Jordan as reservoirs of ticks and tick-borne hemopathogens

et al. 2012

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“…The disease has been reported with increasing frequency in the United States and several European and Asian countries since the first case was reported in the United States in 1994 (3,7,9,12,15,18). A. phagocytophilum DNA has been detected in ticks collected in Israel, Japan, China, and elsewhere (8,14,16). HGA was first reported in China in 2008, and the first HGA outbreak was noteworthy because it caused nosocomial infection (20).…”

mentioning

confidence: 99%

Expression and Immunogenicity of Recombinant Immunoreactive Surface Protein 2 of Anaplasma phagocytophilum

Qiang

Chen

et al. 2012

Clin Vaccine Immunol

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ABSTRACTHuman granulocytic anaplasmosis (HGA), caused byAnaplasma phagocytophilum, is an emerging tick-borne zoonotic disease throughout the world. The first HGA cases in China were documented in 2008, and the greatest challenge posed by the disease is rapid and accurate diagnosis during the acute phage of illness. In this study, we successfully cloned and expressed anA. phagocytophilumimmunoreactive surface protein (major surface protein 2 [MSP2]) and demonstrated that this recombinant protein has natural immunogenicity by Western blotting and enzyme-linked immunosorbent assay (ELISA) using human HGA-positive sera and reference rabbit HGA-positive sera. The rabbit antisera against the recombinant protein also reacted actively with the natural antigen ofA. phagocytophilumby immunofluorescence assay (IFA). No cross-reaction was observed between the recombinant protein and rabbit antisera against 10 common members of the orderRickettsialesby ELISA when the sera were diluted more than 1:100. We concluded that the recombinant MSP2 protein exhibited excellent antigenicity and specificity, results that should lay the foundation for the development of a simple and rapid diagnostic reagent and a vaccination for anaplasmosis.

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Molecular Evidence forAnaplasma phagocytophilumin Israel

Cited by 26 publications

References 15 publications

Seroprevalence of Human Granulocytotropic Anaplasmosis in Central and Southeastern China

Seroprevalence of Human Granulocytotropic Anaplasmosis in Central and Southeastern China

Stray dogs of northern Jordan as reservoirs of ticks and tick-borne hemopathogens

Expression and Immunogenicity of Recombinant Immunoreactive Surface Protein 2 of Anaplasma phagocytophilum

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