Molecular evidence for repertoire skewing of T large granular lymphocyte proliferation after allogeneic hematopoietic SCT: report of two cases

Spontaneous Regression of Methotrexate-related Lymphoproliferative Disorder with T-cell Large Granular Lymphocytosis

“…LGL lymphocytosis is also related to the T-cell receptor β-chain (TRB) gene repertoire (20). In the present case, the patient may have had alleles prone to LGL lymphocytosis and a TRB gene repertoire associated with DLBCL antigen recognition; MTX withdrawal may have induced an increase in the plasma level of IL-6 and activation of STAT3, resulting intransient T-LGL lymphocytosis.…”

T-Cell Dynamics in Chronic Lymphocytic Leukemia under Different Treatment Modalities

“…In order to obtain evidence as to whether the herin identified T cell clones might have expanded in response to CLL-associated antigens or not, we performed extensive comparisons against not only CLL/MBL sequence datasets, 36,41 but also datasets from common herpes virus infections (CMV/EBV), healthy individuals, and entities mediated by T cell clones. 30,[32][33][34][35][37][38][39][40] and found a significant number of major T cell clonotypes shared exclusively among CLL patients. This is remarkable given: (i) the random HLA background of our cohort, and (ii) the size of the comparison dataset (761,968 distinct TRBV-TRBD-TRBJ sequences).…”

“…10 major clonotypes of each sample removing duplicate values such as clonotypes which persisted in overtime analysis of the same patient or clonotypes shared among different patients) and compared them across CLL patients of this cohort, and against: (i) all T cell clonotypes from our previous NGS study involving treatment-naive CLL patients (n=1,105,728), 28 (ii) T cell clonotypes from 15 healthy donors (NGS study by our group, n=573,651), 32 (iii) HHV-4 (Ebstein-Barr virus, EBV), HHV-5 (cytomegalovirus, CMV), and BK virus-specific T cell clonotypes (anti-viral T cell NGS study by our group, n=169,502), 33 (iv) nonredundant, well-annotated, unique T cell clonotypes retrieved from public databases (n=17,024), 34,35 and (v) T cell clonotypes from previous low-throughput immunoprofiling studies (classic subcloning followed by Sanger sequencing) in clinical monoclonal B cell lymphocytosis (high-count MBL, n=545), 36 as well as clinical entities associated with clonal T cell expansions [R-LON (n=283), 30 chronic idiopathic neutropenia (CIN, n=576), 37 large granular T cell leukemia (Τ-LGL, n=932)]. [38][39][40]…”

The Value of Flow Cytometry Clonality in Large Granular Lymphocyte Leukemia

“…From selected articles ( n = 20), data were collected into a standardized form including publication year, source, number of total patients and divided by sex, number of subjects with autoimmune disorders, hematological malignancies, or cancers, and Vβ usage by flow cytometry ( Table 2 ) [ 41 , 42 , 46 , 75 , 76 , 88 , 89 , 90 , 91 , 92 , 93 , 94 , 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 ]. A total of 533 T-LGL leukemia patients were evaluable for Vβ usage analysis, and sex was available in 524 subjects: 308 of them were males (58.8%) and 216 females (41.2%) with a male:female ratio of 1.4, as already described in smaller cohorts [ 1 ].…”