Molecular evidence of an isolate of mungbean yellow mosaic India virus with a recombinant DNA B component occurring on mungbean from mid-hills of Meghalaya, India

Abstract

A new isolate (Mg-mungbean-1) of yellow mosaic virus (YMV) was identified and characterized from mungbean growing in mid-hill condition of Meghalaya, India. Full genome of components (DNA A and DNA B; NCBI accessions number KU95030 and KU95031, respectively) of the virus were amplified through rolling circle amplification and sequenced. Both, DNA A and DNA B shared a common region (CR) with 90.4% similarity. The DNA A of Mg-mungbean-1 showed maximum (97.59%) nucleotide identity with mungbean yellow mosaic India virus (MYMIV) isolate (HF922628) reported from West Bengal, India and DNA B showed ~ 96% nucleotide identity with mungbean yellow mosaic virus (MYMV) isolates having variant DNA B. Phylogenetic tree of DNA A also identified Mg-mungbean-1 as a MYMIV. Based on DNA B the current isolate grouped with the variant Indian MYMV isolates. One recombination event in the CR of DNA B of Mg-mungbean-1 was detected, where MYMV:India:clonePB1 and MYMIV:India:cloneMBB-B31 have been identified as major and minor parents, respectively. Overall, the current study indicated occurrence of an isolate of MYMIV with a recombinant DNA B component on mungbean from mid-hills of Meghalaya, India. To the best of our knowledge this is the first molecular characterization of YMV from northeast India.

Yellow Mosaic Disease (YMD) of Mungbean (Vigna radiata (L.) Wilczek): Current Status and Management Opportunities

“…Recently, a new isolate (Mg-mungbean-1) of MYMIV having a recombinant DNA-B component was identified from Meghalaya (India). The DNA-A based phylogenetic tree also confirmed this novel isolate as a MYMIV ( Banerjee et al, 2018 ).…”

Genome-wide association studies for earliness, MYMIV resistance, and other associated traits in mungbean (Vigna radiata L. Wilczek) using genotyping by sequencing approach

“…MYMIV resistance in mungbean is a complex trait, and limited efforts have been made to understand it through molecular breeding approaches. Resistance mechanisms may involve hindering viral invasion in the plant cell wall, restricting viral DNA replication, and initiating early antioxidant defense responses ( Rani et al, 2017 ; Banerjee et al, 2018 ; Singh et al, 2020 ). Given the economic significance of mungbean and the devastating impact of YMD on its cultivation, it is crucial to intensify research efforts aimed at unraveling the molecular basis of MYMIV resistance.…”

Influence of meteorological parameters on incidence of mungbean yellow mosaic virus disease in green gram in Assam

“…Cultivation of green gram is limited by a number of biotic and abiotic factors. Among different diseases green gram, Yellow Mosaic Virus Disease has become a major threat in production of green gram in the recent years in India and also in Assam (Banerjee et al, 2018) [5] . The crop loss ranges from 10-100 per cent depending on the genotype of green gram and the stage of crop at infection (Bashir et al, 2006) [6] .…”

“…Epidemiological studies on viral diseases especially those transmitted by insect vectors always have great bearing in establishing the virus-vector, virus-plant, and vector-plant interactions which helps in formulating effective management strategies. Although, the yellow mosaic virus disease of green gram is the most important constraint for production of green gram in Assam (Banerjee et al, 2018) [5] , report on systematic study on epidemiological factors that contribute to the development and rapid spread of the disease is very scanty. Therefore, in the present investigation attempt was made to carry out systematic study on the effect of different meteorological parameters on the incidence of yellow mosaic virus disease in greengram, effect of the disease on growth and yield attributes and to find out suitable sowing time to develop effective management strategy of the disease.…”