Molecular evidence of hybrid zones of Cedrela (Meliaceae) in the Yungas of Northwestern Argentina

Zelener, Noga; Tosto, Daniela Sandra; Oliveira, Luiz Orlando de; Soldati, María Cristina; Inza, María Virginia; Fornes, Luis F.

doi:10.1016/j.ympev.2016.05.020

Cited by 12 publications

(5 citation statements)

References 83 publications

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“…Our inferred chloroplast genome phylogeny from this limited sample of Cedrela shows a general lack of species monophyly, as only C. angustifolia resolved as monophyletic in the five tested Cedrela species. This result is consistent with the known taxonomic complexity in this genus from hybridization [68], presumably paraphyletic taxa [9, 13], and proposed the existence of ‘cryptic’ species [8, 13]. In our sample, chloroplast genomes appear to be more informative for geographic origin than for taxonomic classification.…”

Section: Discussionsupporting

confidence: 90%

Genomic resources for the Neotropical tree genus Cedrela (Meliaceae) and its relatives

2019

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BackgroundTree species in the genus Cedrela P. Browne are threatened by timber overexploitation across the Neotropics. Genetic identification of processed timber can be used to supplement wood anatomy to assist in the taxonomic and source validation of protected species and populations of Cedrela. However, few genetic resources exist that enable both species and source identification of Cedrela timber products. We developed several ‘omic resources including a leaf transcriptome, organelle genome (cpDNA), and diagnostic single nucleotide polymorphisms (SNPs) that may assist the classification of Cedrela specimens to species and geographic origin and enable future research on this widespread Neotropical tree genus.ResultsWe designed hybridization capture probes to enrich for thousands of genes from both freshly preserved leaf tissue and from herbarium specimens across eight Meliaceae species. We first assembled a draft de novo transcriptome for C. odorata, and then identified putatively low-copy genes. Hybridization probes for 10,001 transcript models successfully enriched 9795 (98%) of these targets, and analysis of target capture efficiency showed that probes worked effectively for five Cedrela species, with each species showing similar mean on-target sequence yield and depth. The probes showed greater enrichment efficiency for Cedrela species relative to the other three distantly related Meliaceae species. We provide a set of candidate SNPs for species identification of four of the Cedrela species included in this analysis, and present draft chloroplast genomes for multiple individuals of eight species from four genera in the Meliaceae.ConclusionsDeforestation and illegal logging threaten forest biodiversity globally, and wood screening tools offer enforcement agencies new approaches to identify illegally harvested timber. The genomic resources described here provide the foundation required to develop genetic screening methods for Cedrela species identification and source validation. Due to their transferability across the genus and family as well as demonstrated applicability for both fresh leaves and herbarium specimens, the genomic resources described here provide additional tools for studies examining the ecology and evolutionary history of Cedrela and related species in the Meliaceae.Electronic supplementary materialThe online version of this article (10.1186/s12864-018-5382-6) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 90%

Genomic resources for the Neotropical tree genus Cedrela (Meliaceae) and its relatives

2019

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“…Between two Brahea spp., Ramírez-Rodríguez et al used nuclear loci, RAPD data and morphology to recover a mosaic of parental, transgressive and intermediate phenotypes and some asymmetric introgression between rarer and more common species. Comparably, in species of Cedrela P.Browne (Meliaceae) from the yungas forests of Bolivia and Argentina, AFLP data recovered a wide range of hybrid ancestries, suggesting persistent hybridization over generations in multiple hybrid zones (Zelener et al, 2016). Moreover, species of Pinus L. found in similar montane woodland communities show comparable levels of introgression when examined with microsatellite data (Delgado et al, 2007).…”

Section: Montane Forests and Woodlandsmentioning

confidence: 88%

Hybridization: a ‘double-edged sword’ for Neotropical plant diversity

Schley

Twyford

Pennington

2021

Botanical Journal of the Linnean Society

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Hybridization can facilitate both evolutionary diversification and extinction and has had a critical role in plant evolution, with c. 25% of species known to hybridize in some temperate floras. However, in the species-rich Neotropical flora, the role of hybridization in the evolution of diversity remains unclear. Our review examines studies of hybridization in seed plants from across the Neotropics and explores its outcomes on Neotropical plant evolution. We review studies on a per-biome basis and a spectrum of evolutionary outcomes from hybridization are evident across Neotropical biomes and taxa. These range from short-term impacts, such as the broadening of ecological amplitude in hybrid progeny with transgressive phenotypes and genetic swamping, through to long term impacts, such as the generation of new lineages. Among these studies certain themes emerge, such as the pervasive hybridization among species-rich plant radiations from the Andean páramos, suggesting a role for hybridization in rapid diversification events. Finally, we highlight that hybridization is relatively understudied in the Neotropical flora, despite its remarkable species richness. The advent of genomic techniques can facilitate the study of hybridization and its effects in understudied biomes and plant groups. The increasing availability of genomic resources will eventually allow comparisons between tropical and temperate floras and therefore shed light on the evolutionary impacts of hybridization across the latitudinal biodiversity gradient.

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“…In addition, the parental species were well recognized even in a robust dataset containing 13 different species. The applicability of this technique for studying hybridization events was demonstrated recently by Čertner et al (2015), Hulber et al (2015), Szczepaniak et al (2016), Zelener et al (2016), An et al (2017), Píšová et al (2017) and others. In the genus Cirsium it was also used for the molecular confirmation of a new hybrid by Segarra-Moragues et al (2007).…”

Section: Genetic Analysesmentioning

confidence: 77%

Cirsium ×sudae

Michálková¹,

Šmerda²,

Knoll³

et al. 2018

Preslia

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In this study we describe a new nothospecies, Cirsium ×sudae Michálková et Bureš, a homoploid hybrid between two rare Alpine species, C. carniolicum and C. greimleri. Hybrid status was confirmed for four morphologically intermediate individuals, found in the Ennstal Alps, Austria (three of them were F1 hybrids and one a backcross with C. carniolicum). We used amplified fragment length polymorphism (AFLP) to confirm affiliation to the parental species and exclude the potential contribution of other sympatric species. In addition, we used flow cytometry to confirm the diploid status of this hybrid. The newly assessed genome size of this hybrid is 2C = 1.99±0.03 pg, and for C. carniolicum 2C = 2.03±0.04 pg.

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Molecular evidence of hybrid zones of Cedrela (Meliaceae) in the Yungas of Northwestern Argentina

Cited by 12 publications

References 83 publications

Genomic resources for the Neotropical tree genus Cedrela (Meliaceae) and its relatives

Genomic resources for the Neotropical tree genus Cedrela (Meliaceae) and its relatives

Hybridization: a ‘double-edged sword’ for Neotropical plant diversity

Cirsium ×sudae

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