Molecular Evolution of RNA-Dependent RNA Polymerase Region in Norovirus Genogroup I

Zhou, Nan; Huang, Yue; Lu, Zengjun; Li, Mingma; Jin, Hui

doi:10.3390/v15010166

Cited by 6 publications

(3 citation statements)

References 48 publications

(59 reference statements)

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“…Furthermore, both GII.P6-GII.6 and GII.P7-GII.6 RdRp proteins had a relatively small number of amino acid substitutions and no identifiable positive selection site. These results are compatible with previous reports investigating other genogroups (GI) and genotypes [ 42 , 55 ]. This is partly because RdRp, which is not a target of neutralising antibodies, undergoes less selective pressure from host immune systems than VP1.…”

Section: Discussionsupporting

confidence: 93%

Molecular Evolutionary Analyses of the RNA-Dependent RNA Polymerase (RdRp) Region and VP1 Gene in Human Norovirus Genotypes GII.P6-GII.6 and GII.P7-GII.6

Takahashi

Kimura

Shirai

et al. 2023

Viruses

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To understand the evolution of GII.P6-GII.6 and GII.P7-GII.6 strains, the prevalent human norovirus genotypes, we analysed both the RdRp region and VP1 gene in globally collected strains using authentic bioinformatics technologies. A common ancestor of the P6- and P7-type RdRp region emerged approximately 50 years ago and a common ancestor of the P6- and P7-type VP1 gene emerged approximately 110 years ago. Subsequently, the RdRp region and VP1 gene evolved. Moreover, the evolutionary rates were significantly faster for the P6-type RdRp region and VP1 gene than for the P7-type RdRp region and VP1 genes. Large genetic divergence was observed in the P7-type RdRp region and VP1 gene compared with the P6-type RdRp region and VP1 gene. The phylodynamics of the RdRp region and VP1 gene fluctuated after the year 2000. Positive selection sites in VP1 proteins were located in the antigenicity-related protruding 2 domain, and these sites overlapped with conformational epitopes. These results suggest that the GII.6 VP1 gene and VP1 proteins evolved uniquely due to recombination between the P6- and P7-type RdRp regions in the HuNoV GII.P6-GII.6 and GII.P7-GII.6 virus strains.

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Section: Discussionsupporting

confidence: 93%

Molecular Evolutionary Analyses of the RNA-Dependent RNA Polymerase (RdRp) Region and VP1 Gene in Human Norovirus Genotypes GII.P6-GII.6 and GII.P7-GII.6

Takahashi

Kimura

Shirai

et al. 2023

Viruses

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“…Studies on norovirus molecular evolution based on genomic sequences from public databases can provide valuable information for epidemiology, vaccine design, and detection. However, most of previous reports only focused on one specific region, such as VP1, RdRp, and protease gene of norovirus GI and GII 41,51–54 . In this study, besides the molecular characterizations of GII.6 VP1 gene, we also evaluated the genetic diversity of other nonstructural proteins and explored the influence of RdRp region on the molecular evolution of GII.6 VP1 gene.…”

Section: Discussionmentioning

confidence: 99%

“…However, most of previous reports only focused on one specific region, such as VP1, RdRp, and protease gene of norovirus GI and GII. 41,[51][52][53][54] In this study, besides the molecular characterizations of GII.6 VP1 gene, we also Methodology; formal analysis; data curation.…”

Section: Discussionmentioning

confidence: 99%

Genetic characterizations and molecular evolution of human norovirus GII.6 genotype during the past five decades

Zhou

et al. 2023

Journal of Medical Virology

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The norovirus genotype GII.6 is circulating in the population with a relatively high prevalence, but in‐depth molecular characterization studies of GII.6 are needed. In this study, norovirus GII.6 sequences were retrieved and analyzed to demonstrate the molecular characterizations of norovirus GII.6. The results show that GII.6 VP1 gene can be divided into three variants and all variants cocirculated in humans in the past decades. The intragenotypic showed no growth trend over time. Since the overall evolutionary rate was 3.432 × 10−3 substitutions/site/year, the infer most recent common ancestor was estimated as 1913. Only a few amino acid sites were recognized to be under positive selection pressure. The mean effective population size was stable in the recent years. The variant C (especially 87 GII.P7‐GII.6 strains) had a higher evolutionary rate and more sites under positive selection pressure compared to other variants. NS4 protein had the higher diversity than other nonstructural proteins, and VP1 and VP2 genes had the same phylogenetic relationships. This study provides a systematic description of genetic characterizations and molecular evolution of GII.6. Research on norovirus molecular epidemiology should be pursued to enrich the genomic data of the diverse genotypes and improve their analysis.

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Epidemiological and genetic characteristics of norovirus in Hangzhou, China, in the postepidemic era

Chen,

Shao,

et al. 2024

Journal of Clinical Virology

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Molecular Evolution of RNA-Dependent RNA Polymerase Region in Norovirus Genogroup I

Cited by 6 publications

References 48 publications

Molecular Evolutionary Analyses of the RNA-Dependent RNA Polymerase (RdRp) Region and VP1 Gene in Human Norovirus Genotypes GII.P6-GII.6 and GII.P7-GII.6

Molecular Evolutionary Analyses of the RNA-Dependent RNA Polymerase (RdRp) Region and VP1 Gene in Human Norovirus Genotypes GII.P6-GII.6 and GII.P7-GII.6

Genetic characterizations and molecular evolution of human norovirus GII.6 genotype during the past five decades

Epidemiological and genetic characteristics of norovirus in Hangzhou, China, in the postepidemic era

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