Molecular genetic analysis of subterranean clover-microbe interactions

Rolfe, B. G.; Djordjevic, Michael A.; Weinman, Jeremy J.; McIver, J.; Gärtner, E.; Chen, H.; Creaser, E. H.; Britt, Kath A.; Lawson, Charles; Boer, Margreet H. de; McKay, Ian; Shoobridge, Maryanne; Majnik, J. de; Pittock, C.; Broderick, Kathleen Marie; Delbridge, T.

doi:10.1016/0038-0717(95)98623-v

Cited by 3 publications

(2 citation statements)

References 24 publications

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“…Also, in the field, TA1 is known for its failure to nodulate cultivar Woogenellup (Gibson, 1968). Strain TAl still initiates cortical cell division and infection threads containing rhizobia, but subsequent steps are not sustained and nodulation development terminates (Rolfe et al, 1995). A single recessive host gene rwtl was reported to be involved in this cultivarspecific nodulation (Lewis-Henderson and Djordjevic, 1991a).…”

Section: Cultivar-specific Interactions Between R Leguminosarum Bv mentioning

confidence: 97%

“…trifolii ANUS43 also suppresses nodulation of cultivar Woogenellup by strain TA1, whereas ANU843 itself can form an efficient symbiosis with this cultivar, demonstrates that other determinants are involved in this cultivar-specific nodulation. Recently, Rolfe et al (1995) reported that the lipooligosaccharide molecules produced by TA1 are structurally different from those produced by ANU843. The nodT gene of ANU843 (which is naturally deleted in TA1) was shown to act as a dominant suppressor of these negatively acting factors (nodM and csnl).…”

Section: Cultivar-specific Interactions Between R Leguminosarum Bv mentioning

confidence: 99%

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Factors Influencing Nodule Occupancy by Inoculant Rhizobia

Vlassak

Vanderleyden

1997

Critical Reviews in Plant Sciences

115

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Section: Cultivar-specific Interactions Between R Leguminosarum Bv mentioning

confidence: 97%

Section: Cultivar-specific Interactions Between R Leguminosarum Bv mentioning

confidence: 99%

Factors Influencing Nodule Occupancy by Inoculant Rhizobia

Vlassak

Vanderleyden

1997

Critical Reviews in Plant Sciences

115

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Molecular Breeding of Forage Legumes for Virus Resistance

Kalla

Chu

Spangenberg

2001

Developments in Plant Breeding

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Defective Infection and Nodulation of Clovers by Exopolysaccharide Mutants of Rhizobium leguminosarum bv. trifolii

Rolfe¹,

Rw²,

Ridge³

et al. 1996

Functional Plant Biol.

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The biological and symbiotic properties of two types of exopolysaccharide deficient (Exo- phenotype) mutants of Rhizobium leguminosarum were investigated. One mutant, a pss1 derivative (ANU437) of R. l. bv. trifolii, produces very low levels of acidic exopolysaccharide (EPS) and of the acidic oligosaccharide (AOS) repeating unit lacking its O-acetyl substituent. The second strain, originally a R. I. bv. viciae derivative, but containing a clover pSym plasmid (Exo-1 mutant ANU54(pBRIAN)), did not produce any EPS polymer or detectable oligosaccharide repeating unit. The Exo- mutants formed no capsules and their growth in laboratory media was comparable to their parent strains but was inhibited by the addition of the phytoalexins medicarpin and kievitone, but not by the phytoalexin pisatin. Both Exo- mutants were slow to induce clover root hair curling (Hac+), and formed growing infection threads (Inf-) only on white clovers. However, the extent of these early symbiotic defects depended on the plant growth conditions used. Both mutants induced small non-nitrogen-fixing (Fix-) nodules on white and subterranean clovers. Within the white clover nodules induced by mutant ANU437, bacteria were released into plant cells. On white clovers at elevated light and temperature conditions, the Exo-1 mutant ANU54(pBRIAN) usually failed to initiate infection threads and thus formed empty nodules which do not contain any bacteria. In addition, this mutant formed only empty nodules without infection threads on subterranean clovers under all growth conditions tested. The outermost layers of plant cells in these empty nodules had thickened cell walls and deposited materials. These experiments show that EPS synthesis is essential for the induction of rapid root hair curling, proper infection thread formation and the ability to sustain the growth of induced nodules on various clovers.

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Molecular genetic analysis of subterranean clover-microbe interactions

Cited by 3 publications

References 24 publications

Factors Influencing Nodule Occupancy by Inoculant Rhizobia

Factors Influencing Nodule Occupancy by Inoculant Rhizobia

Molecular Breeding of Forage Legumes for Virus Resistance

Defective Infection and Nodulation of Clovers by Exopolysaccharide Mutants of Rhizobium leguminosarum bv. trifolii

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