Molecular-Genetic Approaches to Identification and Typing of Pathogenic Candida Yeasts

Trtková, J.; Raclavsky, Vladislav

doi:10.5507/bp.2006.005

Cited by 26 publications

(25 citation statements)

References 97 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…EK has been extensively used to fingerprint C. albicans and other Candida species (e.g. C. lusitaniae, C. parapsilosis, C. tropicalis and C. glabrata) [14], [19]. It has a moderate discriminatory power when used for typing unrelated C. albicans isolates and does not allow the discrimination between moderately related isolates nor the detection of microevolutionary changes in the same strain.…”

Section: Methods For Identification Of Candida Speciesmentioning

confidence: 99%

See 1 more Smart Citation

Molecular-Genetic Approaches for Identification and Typing of Pathogenic Candida Yeasts: A Review

Singh¹

2014

IJIRSET

View full text Add to dashboard Cite

Candida is a medically important fungi because of its high frequency as a commensal and pathogenic microorganism causing superficial as well as invasive infections. Because the accurate diagnosis of candidiasis remains difficult, a fast and reliable assay for characterization of fungal pathogens is critical for the early initiation of adequate antifungal therapy and/or for introduction of preventive measures. As novel molecular genetic techniques are continuously introduced, their role in the management of infectious diseases has also been growing .Strain typing and delineation of the species are essential for understanding its biology, epidemiology and population structure. A wide range of molecular techniques have been used for this purpose including non-DNA-based methods (multi-locus enzyme electrophoresis), conventional DNA-based methods (electrophoretic karyotyping, random amplified polymorphic DNA, amplified fragment length polymorphism, restriction enzyme analysis with and without hybridization, rep-PCR) and DNA-based methods called exact typing (PCR based methods, microstallite length polymorphism, multilocus sequence typing, DNA microarray ) because they generate unambiguous and highly reproducible typing data. Today, molecular strategies complement conventional methods and provide more accurate and detailed insight. It can be expected that future technical development will improve their potential furthermore.In this article, we provide a critical review on the vigorously fermenting field of molecular approaches to Candida identification and typing and summarized their advantages and limitations with regard to their discriminatory power, reproducibility, cost and ease of performance.

show abstract

Section: Methods For Identification Of Candida Speciesmentioning

confidence: 99%

“…Review papers on the molecular biology of C. albicans are available [7], [12], [13], [14], [15] which address the current advances made in the understanding of C. albicans molecular genetics. In addition, a very useful WWW Server on C. albicans research (http://alces.med.umn.edu/candida.html) has been created by Dr. Scherer (Univ.…”

Section: Related Workmentioning

confidence: 99%

Molecular-Genetic Approaches for Identification and Typing of Pathogenic Candida Yeasts: A Review

Singh¹

2014

IJIRSET

View full text Add to dashboard Cite

show abstract

“…So, chances to occur false-positive results due to contamination, and intense labor are major drawbacks of this technique (Rahman et al 2013). To overcome these limitations, laboratories must follow some strict precautions as to use the separate equipment and place for each PCR cycle (Trtkova and Raclavsky 2006). Orchid disease caused by Phytophthora spp.…”

Section: Nested Pcrmentioning

confidence: 99%

Recent advances in molecular techniques for the identification of phytopathogenic fungi – a mini review

Aslam

Tahir

Aslam

et al. 2017

Journal of Plant Interactions

View full text Add to dashboard Cite

At present, 1.5 million species of fungi are estimated. Among these less than 5% have been described. Many fungal species cause disease in plants. These diseases cause major economic and production losses in the agricultural industry worldwide. Monitoring plant health and detecting the pathogen early are essential to reduce the disease spread, and facilitate effective management practices. DNA-based methods now provide essential tools for accurate plant disease diagnosis. Recently, effective amplification platforms, probe development, various quantitative PCR, DNA barcoding and RNA-Seq-based next-generation sequencing have revolutionized the research in fungal detection field, and differentiation area. Although the molecular diagnostics techniques have grown extensively over the last couple of decades but still there is a long way to go in the development and application of molecular diagnostics to assist the plant disease diagnosticians. Finally, molecular diagnostic techniques used in plant disease diagnostic clinics need to be robust, reliable, inexpensive and easy to be used that they can compete with, and complement traditional techniques. Challenge now remains residue with the researchers to develop the practical techniques used for diagnostic setting. Examples of the recent advancement in the molecular techniques for diagnosing the fungi causing plant disease are discussed in the review.

show abstract

“…Techniques include PCR, real-time PCR, electrophoretic karyotyping, restriction fragment length polymorphism, fluorescence in situ hybridization, randomly amplified polymorphic DNA analysis, multilocus sequence typing and pyrosequencing (Dassanayake & Samaranayake, 2003;Moter & Göbel, 2000;Trtkova & Raclavsky, 2006). However, each technique has its own advantages and limitations, with many requiring a culture step to isolate the target species (Trtkova & Raclavsky, 2006).…”

Section: Introductionmentioning

confidence: 99%

“…Molecular techniques provide more accurate methods for identifying and fingerprinting micro-organisms (Borman et al, 2008;Kuba, 2008;Neppelenbroek et al, 2006;Sidrim et al, 2010;Trtkova & Raclavsky, 2006), and have been used widely to identify and type yeast. Techniques include PCR, real-time PCR, electrophoretic karyotyping, restriction fragment length polymorphism, fluorescence in situ hybridization, randomly amplified polymorphic DNA analysis, multilocus sequence typing and pyrosequencing (Dassanayake & Samaranayake, 2003;Moter & Göbel, 2000;Trtkova & Raclavsky, 2006).…”

Section: Introductionmentioning

confidence: 99%

Use of denaturing gradient gel electrophoresis for the identification of mixed oral yeasts in human saliva

Weerasekera

Sissons

Wong

et al. 2013

Journal of Medical Microbiology

View full text Add to dashboard Cite

A PCR-denaturing gradient gel electrophoresis (DGGE) method was established for the simultaneous presumptive identification of multiple yeast species commonly present in the oral cavity. Published primer sets targeting different regions of the Saccharomyces cerevisiae 26-28S rRNA gene (denoted primer sets N and U) and the 18S rRNA gene (primer set E) were evaluated with ten Candida and four non-Candida yeast species, and twenty Candida albicans isolates. Optimized PCR-DGGE conditions using primer set N were applied to presumptively identify, by band matching, yeasts in the saliva of 25 individuals. Identities were confirmed by DNA sequencing and compared with those using CHROMagar Candida culture. All primer sets yielded detectable DGGE bands for all species tested. Primer set N yielded mainly single bands and could distinguish all species examined, including differentiating Candida dubliniensis from C. albicans. Primer set U was less discriminatory among species but yielded multiple bands that distinguished subspecies groups within C. albicans. Primer set E gave poor yeast discrimination. DGGE analysis identified yeasts in 17 of the 25 saliva samples. Six saliva samples contained two yeast species: three contained C. albicans and three C. dubliniensis. C. dubliniensis was present alone in one saliva sample (total prevalence 16 %). CHROMagar culture detected yeasts in 16 of the yeast-containing saliva samples and did not enable identification of 7 yeast species identified by DGGE. In conclusion, DGGE identification of oral yeast species with primer set N is a relatively fast and reliable method for the simultaneous presumptive identification of mixed yeasts in oral saliva samples.

show abstract

Molecular-Genetic Approaches to Identification and Typing of Pathogenic Candida Yeasts

Cited by 26 publications

References 97 publications

Molecular-Genetic Approaches for Identification and Typing of Pathogenic Candida Yeasts: A Review

Molecular-Genetic Approaches for Identification and Typing of Pathogenic Candida Yeasts: A Review

Recent advances in molecular techniques for the identification of phytopathogenic fungi – a mini review

Use of denaturing gradient gel electrophoresis for the identification of mixed oral yeasts in human saliva

Contact Info

Product

Resources

About