Molecular-genetic characterisation of a new nematode resistance gene in wheat

Ogbonnaya, Francis C.; Seah, S.; Delibes, A.; Jahier, Joseph; López-Braña, I.; Eastwood, R. F.; Lagudah, Evans

doi:10.1007/s001220051689

Cited by 112 publications

(57 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genes Cre3, Cre6 and Cre8 are ineffective for that pathotype. The extent to what Ha71 reproduction is inhibited, permits to rank Romero et al, 1998;Ogbonnaya et al, 2001;Montes et al, 2003;and unpublished data). The genes Cre1, Cre4 and Cre5 are non-allelic to Cre2 and Cre7 and could be combined in the same genotype reducing the likelihood of virulent pathotypes emerging and increasing the durability of resistance Romero et al, 1998;unpublished data).…”

Section: Pyramiding Resistance Genesmentioning

confidence: 96%

“…DNA and isozyme markers linked to genes Cre2, Cre6, H27 and H30 were developed for marker assisted selection (MAS). Two DNA markers were linked to Cre2 and Cre6 in H-93-8 and H-93-35 lines, respectively (Ogbonnaya et al, 2001;Montes et al, 2003) and two isozyme markers corresponding to homoeologous group 4 were linked to H27 and H30 in H-93-33 and TR-3531 lines, respectively (Delibes et al, 1997;Martín-Sánchez et al, 2003). Markers based on LRR sequences described as major determinants of the specificity of NBS-LRR resistance genes in plants (Meyers et al, 1999;Hammond-Kosack and Parker, 2003) are currently searched.…”

Section: Markers Assisted Selection (Mas)mentioning

confidence: 99%

“…Cre genes from genus Aegilops have been transferred to wheat: Cre2, Cre5 and Cre6 from Ae. ventricosa Jahier et al, 1996;Ogbonnaya et al, 2001), Cre3 and Cre4 from Aegilops tauschii (Eastwood et al, 1991(Eastwood et al, , 1994 and Cre7 from Aegilops triuncialis (Romero et al, 1998).…”

mentioning

confidence: 99%

See 2 more Smart Citations

Characterization and selection of hexaploid wheats containing resistance to Heterodera avenae or Mayetiola destructor introgressed from Aegilops: a review

Delibes¹,

Braña²,

Vázquez³

2008

Span. j. agric. res.

Self Cite

View full text Add to dashboard Cite

Cereal cyst nematode (CCN, Heterodera avenae) and Hessian fly (HF, Mayetiola destructor) are two major pests affecting wheat crops worldwide including important cereal areas of Spain. Aegilops ventricosa and Ae. triuncialis were used as donors in a strategy to introduce resistance genes (RG) for these pests in hexaploid wheat (Triticum aestivum L.). Two 42 chromosomes introgression lines have been derived from Ae. ventricosa: H-93-8 and H-93-33 carrying genes Cre2 and H27 conferring resistance to CCN and HF, respectively. Line TR-3531 with 42 chromosomes has been derived from Ae. triuncialis and carries RGs conferring resistance for CCN (Cre7) and for HF (H30). Alien material has been incorporated in lines H-93 by chromosomal substitution and recombination, while in line TR-3531 homoeologous recombination affecting small DNA fragments has played a major role. It has been demonstrated that Cre2, Cre7, H27 and H30 are major single dominant genes and not allelic of other previously described RGs. Biochemical and molecular-biology studies of the defense mechanism triggered by Cre2 and Cre7 have revealed specific induction of peroxidase and other antioxidant enzymes. In parallel to these basic studies advanced lines carrying resistance genes for CNN and/or HF have been developed. Selection was done using molecular markers for eventually «pyramiding» resistance genes. Several isozyme and RAPD markers have been described and, currently, new markers based on transposable elements and NBS-LRR sequences are being developed. At present, two advanced lines have already been included at the Spanish Catalogue of Commercial Plant Varieties.Additional key words: Aegilops triuncialis, Aegilops ventricosa, cereal cyst nematode, Hessian fly, resistance genes, Triticum aestivum. Resumen Revisión. Caracterización y selección de trigos hexaploides con resistencia a Heterodera avenae o Mayetiola destructor transferida desde AegilopsEl nematodo del quiste de los cereales (CCN, Heterodera avenae) y el mosquito del trigo (HF, Mayetiola destructor) son dos plagas que afectan a los cultivos de trigo incluyendo importantes áreas cerealistas en España. Aegilops ventricosa y Ae. triuncialis se han usado como donantes en una estrategia para introducir genes de resistencia (RG) para estas plagas en trigo hexaploide (Triticum aestivum L.). Por una parte, a partir de Ae. ventricosa se han generado dos líneas de introgresión de 42 cromosomas: H-93-8 y H-93-33, con los genes Cre2 y H27 que confieren resistencia a CCN y HF, respectivamente. Por otra, se ha generado la línea TR-3531 de 42 cromosomas, derivada de Ae. triuncialis, con RGs para CCN (Cre7) y HF (H30). La introgresión en las líneas H-93 se ha producido por sustitución cromosómica y recombinación, mientras que en la línea TR-3531 ha sido fundamentalmente por la recombinación homeóloga de pequeños fragmentos de ADN. Cre2, Cre7, H27 y H30 son genes dominantes simples, no alélicos a otros RGs previamente descritos. Estudios bioquímicos y de biología molecular sobre las defensas induci...

show abstract

Section: Pyramiding Resistance Genesmentioning

confidence: 96%

Section: Markers Assisted Selection (Mas)mentioning

confidence: 99%

See 1 more Smart Citation

Characterization and selection of hexaploid wheats containing resistance to Heterodera avenae or Mayetiola destructor introgressed from Aegilops: a review

Delibes¹,

Braña²,

Vázquez³

2008

Span. j. agric. res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Young leaves were harvested from five plants per genotype, frozen in liquid nitrogen and stored at -80°C prior to DNA extraction. Genomic DNA was extracted according to Ogbonnaya et al (2001). A 100 ng mL -1 DNA from each sample was used for whole-genome profiling using DArT markers by Triticarte Pty Ltd, Yarralumla, Australia (http://www.triticarte.com.au/).…”

Section: Genotypingmentioning

confidence: 99%

Molecular Marker Information in the Analysis of Multi‐Environment Trials Helps Differentiate Superior Genotypes from Promising Parents

Borgognone¹,

Butler²,

Ogbonnaya

et al. 2016

Crop Science

View full text Add to dashboard Cite

P lant breeding programs aim to develop new crop varieties with improved characteristics over existing ones available to farmers. Appropriate methods of selection are crucial to successfully identify the best possible parents for crossing, and to choose the best performing genotypes to progress in the program. Both objectives generally involve evaluating the genotypes under consideration in series of designed experiments across several locations and years.Multi-environment trials (MET) are series of field experiments where genotypes grow under different conditions (environments) determined by combinations of year, location and sometimes management factors (Smith et al., 2001). The statistical analysis of MET data aims to provide reliable and accurate predictions of genotype performance across the target environments and information on specific performance from the interaction of genotypes with the environments (G ´ E) (Smith et al., 2001). Molecular Marker Information in the Analysis of Multi-Environment Trials Helps DifferentiateSuperior Genotypes from Promising Parents M. Gabriela Borgognone,* David G. Butler, Francis C. Ogbonnaya, and M. Fernanda Dreccer ABSTRACTThe statistical analysis of multi-environment trial data aims to provide reliable and accurate predictions of genotype performance across the target environments and information on specific performance from the interaction of genotypes with the environments. Genetic gain can be achieved faster when selections are based on predictions from a model that accounts for the relationships among genotypes rather than from a model that assumes unrelated genotypes. Yield and plant height data from 37 international wheat trials were analyzed using a linear mixed model that accounted for relationships among the genotypes via a genomic relationship matrix G derived from 2487 polymorphic DArT molecular markers for 197 genotypes. The elements of this matrix reflect the actual proportion of the parts of the genome surveyed that is identical by state between pairs of individuals, and including it into the model resulted in generally lower average prediction error variances of individual trials in the analyses. Partitioning the total genetic effects into additive and residual non-additive genetic effects has familiar interpretations for plant breeders and facilitates exploring genotype by environment interactions for additive and total effects. This interpretation is still possible with the form of G used in thispaper. This method of analysis could be readily implemented to accelerate genetic gain by plant breeding programs that have molecular markers for the genotypes under study.

show abstract

“…A resistance locus, Cre , has been identified [8,15] and nine genes resistant to CCN (designated Cre1 , 2,3,4,5,6,7,8 , and R ) have been described, in which Cre1 and Cre8 were derived directly from common wheat [16][17][18][19][20][21][22][23][24]. The Cre3 locus, which was derived from Ae.…”

Section: Introductionmentioning

confidence: 99%

Characterization and expression profiling of a novel cereal cyst nematode resistance gene analog in wheat

et al. 2008

View full text Add to dashboard Cite

-Based on the conserved regions of known resistance genes, an NBS-LRR-type CCN resistance gene analog was isolated from the CCN resistant E-10 near isogenic lines (NILs) of wheat, designated as CreZ (GenBank accession number: EU327996). It contained a complete ORF that was 2775 bp in length and encoded 924 amino acids. Sequence comparison indicated that it shared 92% nucleotide and 87% amino acid identity with those of the known CCN-resistance gene Cre3 and had similar characteristic conserved motifs to those in other established NBS-LRR disease resistance genes. The expression profiling of CreZ indicated that it was specifically expressed in the roots of resistant plants and real-time PCR analysis demonstrated that expression levels drastically increased when the plants were inoculated with cereal cyst nematodes. It could be inferred, then, that CreZ belongs to the NBS-LRR resistance gene family and is a candidate gene for potential resistance to the cereal cyst nematode.

show abstract

Molecular-genetic characterisation of a new nematode resistance gene in wheat

Cited by 112 publications

References 20 publications

Characterization and selection of hexaploid wheats containing resistance to Heterodera avenae or Mayetiola destructor introgressed from Aegilops: a review

Characterization and selection of hexaploid wheats containing resistance to Heterodera avenae or Mayetiola destructor introgressed from Aegilops: a review

Molecular Marker Information in the Analysis of Multi‐Environment Trials Helps Differentiate Superior Genotypes from Promising Parents

Characterization and expression profiling of a novel cereal cyst nematode resistance gene analog in wheat

Contact Info

Product

Resources

About