Molecular-genetic characterization of halotolerant strain <i>Priestia megaterium</i> BIM B-1314D

Abstract

Priestia megaterium BIM В‑1314D is a halotolerant strain able to adapt to osmotic stress. The analysis of a full nucleotide sequence of bacterium P. megaterium BIM В‑1314D has revealed that the genome of the studied strain is represented by one circular chromosome and nine plasmids, deposited in the database of GenBank NCBI under the registration number CP058262–CP058271. The size of the bacterial genome constitutes 5 984 922 base pairs with an average GC content of 37.7 %. The genome contains 6 187 genes where 5 978 were annotated as protein-enconding, 92 – as pseudogenes, 154 – as tRNA genes, 8 – as ncRNA, 47 – as rRNA. The genes responsible for synthesis and transport of betaine and proline osmolytes and transport of potassium ions ensuring the adaptation of strain P. megaterium BIM В‑1314D to osmotic stress were local-ized in the genome. Gene loci were defined encoding production of metabolites involved in the synthesis of phytohormones and polyamines accounting for the growth-promoting microbial ability. Gene clusters determining the synthesis of secondary metabolites, cold and heat shock proteins were revealed in the genome. The genome analysis of strain P. megaterium BIM В‑1314D provides the valuable data on the bacterial culture for stimulation of the plant growth in the salinized conditions.

A cyclic dipeptide for salinity stress alleviation and the trophic flexibility of an endophyte reveal niches in salt marsh plant-microbe interactions

“…, salt marshes, alkaline lakes, potash salt dumps, etc. ), suggesting their immense potential in dealing with such abiotic stresses (79–81).…”

“…Many of them were reported as beneficial endophytes, PGPB, or originally inhabited in alkaline and hypersaline environments (e.g., salt marshes, alkaline lakes, potash salt dumps, etc. ), suggesting their immense potential in dealing with such abiotic stresses (79)(80)(81).…”