Molecular genetic differentiation of avian Escherichia coli by RAPD-PCR

Abstract

Escherichia coli is one of the most important bacterial avian pathogens and a common inhabitant of the gastrointestinal tract of animals. Most pathogenic E. coli can not be differentiated biochemically or by classic microbiologic methods. Molecular typing methods, particularly PCR, facilitated epidemiological and ecological studies of bacteria. Here we describe the application of a random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) for molecular genetic differentiation of E. coli isolates in Iran. In this study 58 E. coli isolates including 4 standard strains, 3 food originated isolates, 33 avian isolates, 8 isolates form diarrheic calves and 10 isolates from unweaned diarrheic lambs were analyzed by RAPD-PCR using primer 1247(5 / -AAG AGC CCG T-3 / ). The RAPD analysis showed that these isolates could be grouped into 33 RAPD types and avian isolates were discriminated into 29 genotypes. It was shown that the primer could not differentiate E. coli isolated from lambs. Discriminatory index for entire isolates was 0.912 and for avian isolates was 0.990. We concluded that RAPD-PCR can be used as a method for molecular differentiation of E. coli isolates.

Genetic and Phylogenetic Diversity of Escherichia coli Strains Collected From Fecal Specimens of Patients Admitted to Zabol Hospitals

“…17 Additionally, Salehi et al concluded that the RAPD can be a useful method for determining the genetic differences between the E. coli isolates. 18 Similar to other studies, the analysis of dendrogram represented high genetic diversity in E.coli isolates, indicating the participation of different pollution centers in spreading the bacteria. 19,20 Similarly, the results of the current study demonstrated that different isolates of the same E. coli species yielded no similar phylogenetic relationship.…”

Effect of Interaction of Methanol Leaf Extract of <i>Spondias mombin</i> (Linn) and Amoxicillin on Some Diarrheagenic <i>Escherichia coli</i>

“…The combined regimen of such natural products with antibiotics for better effectiveness have long been advocated [19]. Rich phytochemical constituents observed in this study are secondary metabolites already reported with protective roles [20].…”

Antimicrobial activity of commonly used antibiotics and DNA fingerprint analysis of Pseudomonas aeruginosa obtained from clinical isolates and unchlorinated drinking water in Korea, 2010

“…And the use of various primers for P. aeruginosa generated a total of 131 amplified fragments of between 200 to 300 bp, this more than the number of fragments generated from Staphylococcus aureus and Escherichia coli while using the same number of primers (Onasanya et al, 2003;Salehi et al, 2008;Shehata, 2008).…”