Molecular Genetics and Cytotoxic Responses to Titanium Diboride and Zinc Borate Nanoparticles on Cultured Human Primary Alveolar Epithelial Cells

Türkez, Hasan; Arslan, Mustafa; Tatar, Arzu; Tozlu, Özlem Özdemir; Sönmez, Erdal; Çadırcı, Kenan; Hacımüftüoğlu, Ahmet; Ceylan, Bahattin; Açıkyıldız, Metin; Kahraman, Çiğdem Yüce; Geyikoğlu, Fatime; Tatar, Abdülgani; Mardinoğlu, Adil

doi:10.3390/ma15072359

Cited by 4 publications

(1 citation statement)

References 52 publications

(49 reference statements)

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“…In this regard, the chemical, physical, and biological features of B provide medicinal chemists a unique chance to research and develop brand new fields of biomedical sciences, especially in drug discovery [23][24][25]. Recent investigations indicated that BCCs could affect the crucial cellular machineries implicating cell survival, tissue regeneration, and immunogenic responses [26]. Although the toxicity potentials by borenium and borinium compounds are not well known, their antibacterial and antiviral properties have been well documented [27][28][29].…”

Section: Introductionmentioning

confidence: 99%

Oxidative, Genotoxic and Cytotoxic Damage Potential of Novel Borenium and Borinium Compounds

Oguzkan,

Turkez,

Ugras

et al. 2023

Inorganics

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In this study, the biological properties of novel borenium and borinium compounds in terms of their oxidative, genotoxic, and cytotoxic effects were assessed on cultured human peripheral blood cells, as well as several types of cancer cells. Our results revealed that the borinium compounds yielded the best results in terms of supporting total antioxidant capacity (TAC). In fact, borenium 1, borenium 2, borenium 3, borinium 4, and borinium 5 compounds elevated TAC levels of cultured human blood cells at rates of 42.8%, 101.5%, 69.8%, 33.3%, and 49.2%, respectively. There were no statistically significant differences (p > 0.05) between the negative control and the groups treated with all borinium and borenium concentrations from the micronucleus (MN) and chromosome aberration (CA) assays, demonstrating the non-genotoxic effects. Moreover, borenium 1 (60.7% and 50.7%), borenium 2 (70.4% and 57.2%), borenium 3 (53.1% and 45.2%), borinium 4 (55.1% and 48.1%), and borinium 5 (51.0% and 36.1%) minimized the mitomycin C(MMC)-induced genotoxic damages at different rates as determined using CA and MN assays, respectively. Again, it was found that the borinium compounds exhibited higher cytotoxic activity on cancer cells when compared to borenium compounds. Consequently, in light of our in vitro findings, it was suggested that the novel borinium and borenium compounds could be used safely in pharmacology, cosmetics, and various medical fields due to their antioxidant and non-genotoxic features, as well as their cytotoxicity potential on cancer cells.

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Section: Introductionmentioning

confidence: 99%

Oxidative, Genotoxic and Cytotoxic Damage Potential of Novel Borenium and Borinium Compounds

Oguzkan,

Turkez,

Ugras

et al. 2023

Inorganics

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In Vitro Transcriptome Analysis of Cobalt Boride Nanoparticles on Human Pulmonary Alveolar Cells

et al. 2022

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Nanobiotechnology influences many different areas, including the medical, food, energy, clothing, and cosmetics industries. Considering the wide usage of nanomaterials, it is necessary to investigate the toxicity potentials of specific nanosized molecules. Boron-containing nanoparticles (NPs) are attracting much interest from scientists due to their unique physicochemical properties. However, there is limited information concerning the toxicity of boron-containing NPs, including cobalt boride (Co2B) NPs. Therefore, in this study, Co2B NPs were characterized using X-ray crystallography (XRD), transmission electron microscope (TEM), scanning electron microscope (SEM), and energy-dispersive X-ray spectroscopy (EDX) techniques. Then, we performed 3-(4,5-dimethyl-thiazol-2-yl) 2,5-diphenyltetrazolium bromide (MTT), lactate dehydrogenase (LDH) release, and neutral red (NR) assays for assessing cell viability against Co2B NP exposure on cultured human pulmonary alveolar epithelial cells (HPAEpiC). In addition, whole-genome microarray analysis was carried out to reveal the global gene expression differentiation of HPAEpiC cells after Co2B NP application. The cell viability tests unveiled an IC50 value for Co2B NPs of 310.353 mg/L. The results of our microarray analysis displayed 719 gene expression differentiations (FC ≥ 2) among the analyzed 40,000 genes. The performed visualization and integrated discovery (DAVID) analysis revealed that there were interactions between various gene pathways and administration of the NPs. Based on gene ontology biological processes analysis, we found that the P53 signaling pathway, cell cycle, and cancer-affecting genes were mostly affected by the Co2B NPs. In conclusion, we suggested that Co2B NPs would be a safe and effective nanomolecule for industrial applications, particularly for medical purposes.

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Cytotoxicity, Corrosion Resistance, and Wettability of Titanium and Ti-TiB2 Composite Fabricated by Powder Metallurgy for Dental Implants

Aljafery,

Fatalla,

Haider

2024

Metals

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Objectives: Orthopedics and dentistry have widely utilized titanium alloys as biomaterials for dental implants, but limited research has been conducted on the fabrication of ceramic particle-reinforced Ti composites for further weight reductions. The current study compared titanium–titanium diboride metal composites (Ti-TiB2) with pure titanium (processed by powder metallurgy) in terms of toxicity, corrosion resistance, and wettability. Methods: First, cell lines of a primary dermal fibroblast normal human adult (HDFa) were used to test the cytocompatibility (in vitro) of the composite and pure Ti using an indirect contact approach. Corrosion testing was performed for the materials using electrochemical techniques such as potentiodynamic polarization in a simulated bodily fluid (SBF) in conjunction with a three-electrode electrochemical cell. The entire set of experimental tests was conducted according to the ASTM F746-04 protocol. The contact angles were measured during wettability testing in accordance with ASTM D7334-08. An X-ray diffractometer (XRD) was used to catalog every phase that was visible in the microstructure. A scanning electron microscope (SEM) and energy-dispersive X-ray spectroscopy (EDS) were used to determine the chemical composition. Results: The cytotoxicity tests revealed that there was no detectable level of toxicity, and there was no significant difference in the impact of either of the two materials on the viability of human fibroblasts. An increase in the corrosion resistance of the composite (0.036 ± 0.0001 mpy (millimeters per year)) demonstrated the development of a passive oxide coating. According to the findings, the composites showed a greater degree of hydrophilicity (contact angle 44.29° ± 0.28) than did the pure titanium (56.31° ± 0.47). Conclusions/Significance: The Ti-TiB2 composite showed no toxicity and better corrosion resistance and wettability than did pure Ti. The composite could be a suitable alternative to Ti for applications involving dental implants.

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Molecular Genetics and Cytotoxic Responses to Titanium Diboride and Zinc Borate Nanoparticles on Cultured Human Primary Alveolar Epithelial Cells

Cited by 4 publications

References 52 publications

Oxidative, Genotoxic and Cytotoxic Damage Potential of Novel Borenium and Borinium Compounds

Oxidative, Genotoxic and Cytotoxic Damage Potential of Novel Borenium and Borinium Compounds

In Vitro Transcriptome Analysis of Cobalt Boride Nanoparticles on Human Pulmonary Alveolar Cells

Cytotoxicity, Corrosion Resistance, and Wettability of Titanium and Ti-TiB2 Composite Fabricated by Powder Metallurgy for Dental Implants

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