Molecular genotype analysis of Salmonella bovismorbificans

Ezquerra, Esther; Burnens, André P.; Frith, K.; Costas, M.; Stanley, John R.

doi:10.1006/mcpr.1993.1006

Cited by 18 publications

(14 citation statements)

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“…In S. Bovismorbificans (serogroup C2) -a veterinary pathogen which also causes sporadic human infections -three clonal lines were characterised, but not all strains carried IS200. The latter strains had unique ribotypes and protein electropherotypes, and their IS200 bands were not conserved -they were minor clones within the serovar [66]. In S. Virchow (serogroup Cl), the third most common serovar isolated from man in the UK, IS200 was found in only 10% of the 57 phage type strains and occurred only in low copy number (1-4) with no conserved bands [67].…”

Section: Salmonella Serovars Of Groups CI and C2mentioning

confidence: 99%

DNA insertion sequences and the molecular epidemiology of Salmonella and Mycobacterium

Stanley

Saunders²

1996

Journal of Medical Microbiology

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The general characteristics and genetics of insertion sequence (IS) elements are wellestablished. For Escherichia coli IS elements, mechanisms of transposition and mutation are known and their recombinogenic role in the bacterial genome has been investigated. Population models relate the distribution of these IS elements to autoregulation of their transposition. IS200, the smallest known element, is confined to the salmonellae and several lineages of E. coli. It exhibits atypical molecular features. The population dynamics of IS200 make it a particularly effective marker of chromosomal genotype in many Salmonella serovars. Molecular epidemiological typing with IS200 has been developed for important serovars in groups D1, C1, C2 and B. Findings for S. Enteritidis, S. Panama, S. Infantis, S. Typhimurium, S. Heidelberg, S. Paratyphi B and S. Java are reviewed. Of the 12 IS elements found in mycobacteria, IS6110, found in Mycobacterium tuberculosis and M. bovis, exhibits the greatest potential for molecular epidemiological applications. Although M. tuberculosis is a single serogroup, and its genome is otherwise highly homogeneous, strains are highly polymorphic with respect to copy number and location of IS6110. A standard IS6110 typing method has been established, together with novel PCR-based approaches to IS611 0 fingerprinting.

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Section: Salmonella Serovars Of Groups CI and C2mentioning

confidence: 99%

DNA insertion sequences and the molecular epidemiology of Salmonella and Mycobacterium

Stanley

Saunders²

1996

Journal of Medical Microbiology

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“…insertion element, found in many isolates of salmonella [4][5][6] with the exception of Agona, some strains of Arizonae [5], Bovismorbificans [7], Dar-essalam [8] and Choleraesuis [9] is another commonly used molecular probe. The use of this insertion sequence epidemiologically and phylogenetically significant observations have been made in several salmonella serovars (i.e.…”

Section: Introductionmentioning

confidence: 99%

“…The use of this insertion sequence epidemiologically and phylogenetically significant observations have been made in several salmonella serovars (i.e. Enteritidis, Bovismorbificans, Abortusovis, Heidelberg, Dublin,Typhimurium, Brandeburg, Typhi) [3,7,[9][10][11][12][13][14][15]. The element is also present in certain isolates of Shigella sp.…”

Section: Introductionmentioning

confidence: 99%

IS200 fingerprint of Salmonella enterica serotype Typhimurium human strains isolated in Sardinia

et al. 1998

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A collection of Salmonella enterica serotype Typhimurium human strains isolated in Northern Sardinia (Italy) was examined for the insertion sequence IS200, phage type, antibiotic profile, ribotyping polymorphisms and plasmid profile. All clinical isolates studied contained from 4 to 10 copies of the IS200 element. IS200 permitted to discriminate Typhimurium strains and to identify five IS200 types, some of them circulating in Sardinia at least since 1900. Strains belonging to phage DT104 predominated and correlated with a specific IS200 pattern.

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“…A number of recent strategies have employed PCR-based approaches to determine different O and H antigens as a means to replace serologic identification of these antigens (14,20,25,32). Others have proposed alternative strategies examining genetic differences as a means of identifying serovars, including ribotyping (17), pulsed-field gel electrophoresis (PFGE) (28), multiplex PCR (3,4,29), IS200 analysis (18,46), random amplification of DNA polymorphisms (45), and DNA microarray analysis (38,42).…”

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confidence: 99%

High-Throughput Molecular Determination of Salmonella enterica Serovars by Use of Multiplex PCR and Capillary Electrophoresis Analysis

Leader

Frye²,

et al. 2009

J Clin Microbiol

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Salmonella enterica is a leading cause of food-borne illness worldwide and is also a major cause of morbidity and mortality in domestic and wild animals. In the current study, a high-throughput molecular assay was developed to determine the most common clinical and nonhuman serovars of S. enterica in the United States. Sixteen genomic targets were identified based on their differential distribution among common serovars. Primers were designed to amplify regions of each of these targets in a single multiplex PCR while incorporating a 6-carboxyfluorescein-labeled universal primer to fluorescently label all amplicons. The fluorescently labeled PCR products were separated using capillary electrophoresis, and a Salmonella multiplex assay for rapid typing (SMART) code was generated for each isolate, based upon the presence or absence of PCR products generated from each target gene. Seven hundred fifty-one blind clinical isolates of Salmonella from Washington State, collected in 2007 and previously serotyped via antisera, were screened with the assay. A total of 89.6% of the isolates were correctly identified based on comparison to a panel of representative SMART codes previously determined for the top 50 most common serovars in the United States. Of the remaining isolates, 6.2% represented isolates that produced a new SMART code for a previously determined serotype, while the final 8.8% were from serotypes not screened in the original panel used to score isolates in the blinded study. This high-throughput multiplex PCR assay allowed simple and accurate typing of the most prevalent clinical serovars of Salmonella enterica at a level comparable to that of conventional serotyping, but at a fraction of both the cost and time required per test.

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Molecular genotype analysis of Salmonella bovismorbificans

Cited by 18 publications

References 0 publications

DNA insertion sequences and the molecular epidemiology of Salmonella and Mycobacterium

DNA insertion sequences and the molecular epidemiology of Salmonella and Mycobacterium

IS200 fingerprint of Salmonella enterica serotype Typhimurium human strains isolated in Sardinia

High-Throughput Molecular Determination of Salmonella enterica Serovars by Use of Multiplex PCR and Capillary Electrophoresis Analysis

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