Molecular genotyping of<i>Echinococcus granulosus</i>using formalin-fixed paraffin-embedded preparations from human isolates in unusual tissue sites

Hizem, Amani; M’rad, Selim; Oudni-M’rad, Myriam; Mestiri, S; Hammedi, F.; Mezhoud, Habib; Zakhama, Abdelfattah; Mokni, M.; Babba, Hamouda

doi:10.1017/s0022149x15000516

Cited by 15 publications

(8 citation statements)

References 30 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The mitochondrial 12S rRNA gene was amplified with PCR using the Egss1 primer set specific to the G1 genotype of E. granulosus s.s. [ 22 ]. The PCR assay was carried out as previously described by Hizem et al [ 23 ]. The non-amplified Egss1 fragments were re-examined targeting a fragment of the mitochondrial Cox1 gene by PCR assay according to the procedure described by Gasser et al [ 24 ].…”

Section: Methodsmentioning

confidence: 99%

Human and animal cystic echinococcosis in Tataouine governorate: hypoendemic area in a hyperendemic country, myth or reality?

et al. 2021

View full text Add to dashboard Cite

Background Cystic echinococcosis (CE) has a worldwide distribution and is especially prevalent in North African countries. With a mean annual surgical incidence (ASI) of CE of 12.7 per 100,000 inhabitants, Tunisia is one of the most CE endemic countries in the Mediterranean area. Tataouine governorate is considered to be the most CE hypoendemic region in Tunisia (ASI = 0.92) despite favourable socioeconomic conditions that enable maintenance of the Echinococcus granulosussensu lato (s.l.) life-cycle and a significant environmental contamination with E. granulosuss.l. eggs. The aim of this study was to assess human CE seroprevalence, prevalence of CE in food animals and environmental contamination by E. granulosus s.l. eggs in different districts of Tataouine governorate. Methods This study was conducted from January to December 2018. A total of 374 human sera samples were tested for the presence of immunoglobulin G antibodies against E. granulosus using a commercial ELISA kit. Specimens were also collected from animals slaughtered at the Tataouine abattoir (n = 8609) and examined for the presence of hydatid cysts; 111 hydatid cysts were genotyped. Eggs of E. granulosuss.l. were identified by PCR and DNA sequencing from dog faecal samples (n = 288). Results Serological tests showed that 8.5% of the sera samples tested were positive for E. granulosus-specific antibodies. The average prevalence of hydatidosis in livestock was 1.6%, and CE infection was more prevalent in cattle than in sheep, goats and dromedaries. The contamination rate of dog faeces by E. granulosus sensu stricto eggs varied significantly from 0 to 23.5% depending on the collection area. Molecular analyses only revealed the presence of the G1 genotype for cysts and eggs. Conclusions Based on our findings, CE is likely to be more endemic in the Tataouine governorate than previously described. Thus, to implement an effective control programme against CE, a national survey should be carried out to determine human CE prevalence in the different Tunisian governorates. Graphic Abstract

show abstract

Section: Methodsmentioning

confidence: 99%

Human and animal cystic echinococcosis in Tataouine governorate: hypoendemic area in a hyperendemic country, myth or reality?

et al. 2021

View full text Add to dashboard Cite

show abstract

“…Низка цестодологів (Thompson and Smyth, 1975;Gemmell, 1978;Smyth, 1979;Thompson, 1979;Thompson and Kumaratilaka, 1982) свого часу намагалися дійти спільної думки щодо таксономічного статусу таких ізолятів, однак робота над цим питанням триває донині. Сучасними молекулярно-генетичним методами виявлено суттєві відмінності структури ДНК внутрішньовидових варіацій ехінококу (Kamenetzky et al, 2002;Erdem et al, 2008;Saarma et al, 2009;Rue et al, 2011;Hanifian et al, 2013;Piccoli et al, 2013;Sharma et al, 2013;Hizem et al, 2016), на основі чого запропоновано розглядати як достовірне існування щонайменше 10 штамів E. granulosus, проте питання надання їм статусу окремих видів або, принаймні, підвидів залишається відкритим.…”

Section: вступunclassified

The physiological state of the larvae and infestation of domestic ungulates by Echinococcus granulosus (Cyclophyllidea, Taeniidae) in North-Eastern Ukraine

2016

View full text Add to dashboard Cite

Echinococcus granulosus (Batsch, 1786) has a worldwide distribution. Different types of carnivores (dogs, jackals, wolves, foxes), domestic and wild ungulates are included in its life cycle. There are significant differences in its pattern of contamination of domestic ungulates in the various regions of Ukraine. Pigs are major intermediate hosts of E. granulosus in the northern and western regions of the country, sheep and cattle in the southern and eastern regions. These differences are associated with strains of Echinococcus. The article presents the results of research conducted on 1,884 pigs, 1,025 sheep and 1,406 cattle in Polesia and the forest-steppe zone of the north-east of Ukraine. We have established the development of Echinococcus larvae in pigs (extent of invasion 10.2 ± 0.6%) and sheep (extent of invasion 0.7 ± 0.2%). The cysts did not develop in cattle. Pigs generally had a low invasion intensity (less than 6 larvae). A high level of invasion intensity (more than 10 larvae) was infrequently recorded in these animals. All sheep had a low level of invasion intensity.The E. granulosus larvae found in sheep were dead. In pigs, most cysts developed to fertile state (139 cases). Young, sterile and dead larvae were less common (39, 10, 4 cases). Pigs older than 10 months had the highest number of fertile cysts. Young larvae dominated in pigs younger than 10 months. In these animals, larvae were localized predominantly in the liver. The kidneys and lungs were infected less frequently. The large number of fertile cysts in pigs older than 10 months made this group of animals the main intermediate hosts of E. granulosus in the north-eastern part of Ukraine. They enable the parasite to function actively here. Analysis of infestation by E. granulosus larvae of domestic ungulates of the Polesia and the forest-steppe zone showed no significant differences. Identified differences were not statistically confirmed.

show abstract

“…The liver is affected in approximately 70% of cases, followed by the lungs (20%). Other organs can be affected such as the abdominal or pleural cavities, kidney, spleen, bone, heart, central nervous system and other sites (Hizem et al ., 2016). Rare immune-mediated reactions such as urticaria, asthma, membranous nephropathy and anaphylaxis may also occur (Agudelo et al ., 2016).…”

Section: Introductionmentioning

confidence: 99%

Efficacy of novel albendazole salt formulations against secondary cystic echinococcosis in experimentally infected mice

et al. 2020

View full text Add to dashboard Cite

In this study, we evaluated the efficacy, expressed as a mean weight decrease of the whole echinococcal cyst mass, of novel benzimidazole salt formulations in a murine Echinococcus granulosus infection model. BALB/c mice were intraperitoneally infected with protoscoleces of E. granulosus (genotype G1). At 9 months post-infection, treatment with albendazole (ABZ), ricobendazole (RBZ) salt formulations, and RBZ enantiomer salts (R)-(+)-RBZ-Na and (S)-(−)-RBZ-Na formulations were initiated. Drugs were orally applied by gavage at 10 mg kg−1 body weight per day during 30 days. Experimental treatments with benzimidazole sodium salts resulted in a significant reduction of the weight of cysts compared to conventional ABZ treatment, except for the (S)-(−)-RBZ-Na enantiomer formulation. Scanning electron microscopy and histological inspection revealed that treatments impacted not only the structural integrity of the parasite tissue in the germinal layer, but also induced alterations in the laminated layer. Overall, these results demonstrate the improved efficacy of benzimidazole salt formulations compared to conventional ABZ treatment in experimental murine cystic echinococcosis.

show abstract

Molecular genotyping ofEchinococcus granulosususing formalin-fixed paraffin-embedded preparations from human isolates in unusual tissue sites

Cited by 15 publications

References 30 publications

Human and animal cystic echinococcosis in Tataouine governorate: hypoendemic area in a hyperendemic country, myth or reality?

Human and animal cystic echinococcosis in Tataouine governorate: hypoendemic area in a hyperendemic country, myth or reality?

The physiological state of the larvae and infestation of domestic ungulates by Echinococcus granulosus (Cyclophyllidea, Taeniidae) in North-Eastern Ukraine

Efficacy of novel albendazole salt formulations against secondary cystic echinococcosis in experimentally infected mice

Contact Info

Product

Resources

About