Molecular Hydrogen Alleviates Cellular Senescence in Endothelial Cells

Hara, Fumihiko; Tatebe, Junko; Watanabe, I.; Yamazaki, Junichi; Ikeda, Takanori; Morita, Toshisuke

doi:10.1253/circj.cj-16-0227

Cited by 31 publications

(23 citation statements)

References 35 publications

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“…Similarly, the long-lasting antioxidant and antiaging effects of molecular hydrogen on ECs treated with 2,3,7,8-tetrachlorodibenzo-p-dioxin, a family of dioxins mediating blood vessel inflammation and premature senescence, occur through the activation of Nrf2 and are paralleled by a positive modulation of SIRT1 activity until 24 h of exposure (64). Although a direct interaction between Nrf2 and SIRT1 has been described in other cell models (87), it has yet to be proven in ECs.…”

Section: Oxidative Stress and Vascular Dysfunctionmentioning

confidence: 99%

SIRT1 and SIRT6 Signaling Pathways in Cardiovascular Disease Protection

D’Onofrio¹,

Servillo²,

Balestrieri³

2018

Antioxidants & Redox Signaling

295

238

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Significance: Oxidative stress represents the common hallmark of pathological conditions associated with cardiovascular disease (CVD), including atherosclerosis, heart failure, hypertension, aging, diabetes, and other vascular system-related diseases. The sirtuin (SIRT) family, comprising seven proteins (SIRT1-SIRT7) sharing a highly conserved nicotinamide adenine dinucleotide (NAD + )-binding catalytic domain, attracted a great attention for the past few years as stress adaptor and epigenetic enzymes involved in the cellular events controlling aging-related disorder, cancer, and CVD. Recent Advances: Among sirtuins, SIRT1 and SIRT6 are the best characterized for their protective roles against inflammation, vascular aging, heart disease, and atherosclerotic plaque development. This latest role has been only recently unveiled for SIRT6. Of interest, in recent years, complex signaling networks controlled by SIRT1 and SIRT6 common to stress resistance, vascular aging, and CVD have emerged. Critical Issues: We provide a comprehensive overview of recent developments on the molecular signaling pathways controlled by SIRT1 and SIRT6, two post-translational modifiers proven to be valuable tools to dampen inflammation and oxidative stress at the cardiovascular level. Future Directions: A deeper understanding of the epigenetic mechanisms through which SIRT1 and SIRT6 act in the signalings responsible for onset and development CVD is a prime scientific endeavor of the upcoming years. Multiple ''omic'' technologies will have widespread implications in understanding such mechanisms, speeding up the achievement of selective and efficient pharmacological modulation of sirtuins for future applications in the prevention and treatment of CVD. Antioxid. Redox Signal. 28, 711-732.

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Section: Oxidative Stress and Vascular Dysfunctionmentioning

confidence: 99%

SIRT1 and SIRT6 Signaling Pathways in Cardiovascular Disease Protection

D’Onofrio¹,

Servillo²,

Balestrieri³

2018

Antioxidants & Redox Signaling

295

238

View full text Add to dashboard Cite

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“…Recent reports showed that hydrogen can remit some serious intestinal diseases with different methods of intake into the body [14][15][16]. In addition, its protective effects were demonstrated in some cell models in vitro with the use of H 2 -saturated medium [32][33][34]. Therefore, we used DON, which is a prevalent mycotoxin in swine production, to induce cell injury to investigate the protective effects of H 2 on oxidative damage and apoptosis in IPEC-J2 cells.…”

Section: Discussionmentioning

confidence: 99%

Protective Role of Hydrogen Gas on Oxidative Damage and Apoptosis in Intestinal Porcine Epithelial Cells (IPEC-J2) Induced by Deoxynivalenol: A Preliminary Study

Zheng

Yao

2019

Toxins

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To explore the protective role of hydrogen gas (H 2 ) on oxidative damage and apoptosis in intestinal porcine epithelial cells (IPEC-J2) induced by deoxynivalenol (DON), cells were assigned to four treatment groups, including control, 5 µM DON, H 2 -saturated medium, and 5 µM DON + H 2 -saturated medium treatments. After 12 h of different treatments, the cell viability, biomarkers of cell redox states, and gene expression of antioxidant enzymes and apoptosis were observed and detected. Furthermore, caspase-3 and Bax protein expressions were measured by Western blot analysis. Our results demonstrated that the 5 µM DON significantly caused cytotoxicity to IPEC-J2 cells by reducing cell viability and increasing lactate dehydrogenase release in culture supernatants. Moreover, DON treatments significantly increased levels of 8-hydroxy-2 -deoxyguanosine, 3-nitrotyrosine, and malonaldehyde; however, they decreased total superoxide dismutase and catalase activities and downregulated messenger RNA (mRNA) expression related to antioxidant enzymes in cells. The 5 µM DON treatment also downregulated Bcl-2 expression and upregulated caspase-3 and Bax expression. However, the H 2 -saturated medium significantly improved cell growth status and reversed the change of redox states and expression of genes and proteins related to apoptosis induced by DON in IPEC-J2 cells. In conclusion, H 2 could protect IPEC-J2 cells from DON-induced oxidative damage and apoptosis in vitro.

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“…When overexpressed in mice, Sirt1 phenocopies the state of calorie restriction with all its beneficial effects, such as improved insulin sensitivity, enhanced mitochondrial biogenesis, and a lower incident of age‐related diseases . For example, recent data suggest that Sirt1 plays an important role in age‐related arterial dysfunction . On a molecular level, Sirt1‐dependent deacetylation and activation of the acetyl‐CoA synthetase 1 (AceCS1) leads to an increase in endogenous fatty acid synthesis .…”

Section: Sirtuin Substrates and Therapeutic Potential Of Sirtuin Modumentioning

confidence: 99%

The Current State of NAD⁺‐Dependent Histone Deacetylases (Sirtuins) as Novel Therapeutic Targets

Schiedel

Robaa

Rumpf

et al. 2017

Medicinal Research Reviews

115

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Abstract:Sirtuins are NAD + -dependent protein deacylases that cleave off acetyl, as well as other acyl groups, from the ε-amino group of lysines in histones and other substrate proteins. Seven sirtuin isotypes (Sirt1-7) have been identified in mammalian cells. As sirtuins are involved in the regulation of various physiological processes such as cell survival, cell cycle progression, apoptosis, DNA repair, cell metabolism, and caloric restriction, a dysregulation of their enzymatic activity has been associated with the pathogenesis of neoplastic, metabolic, infectious, and neurodegenerative diseases. Thus, sirtuins are promising targets for pharmaceutical intervention. Growing interest in a modulation of sirtuin activity has prompted the discovery of several small molecules, able to inhibit or activate certain sirtuin isotypes. Herein, we give an update to our previous review on the topic in this journal , focusing on recent developments in sirtuin biology, sirtuin modulators, and their potential as novel therapeutic agents.

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Molecular Hydrogen Alleviates Cellular Senescence in Endothelial Cells

Cited by 31 publications

References 35 publications

SIRT1 and SIRT6 Signaling Pathways in Cardiovascular Disease Protection

SIRT1 and SIRT6 Signaling Pathways in Cardiovascular Disease Protection

Protective Role of Hydrogen Gas on Oxidative Damage and Apoptosis in Intestinal Porcine Epithelial Cells (IPEC-J2) Induced by Deoxynivalenol: A Preliminary Study

The Current State of NAD⁺‐Dependent Histone Deacetylases (Sirtuins) as Novel Therapeutic Targets

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Molecular Hydrogen Alleviates Cellular Senescence in Endothelial Cells

Cited by 31 publications

References 35 publications

SIRT1 and SIRT6 Signaling Pathways in Cardiovascular Disease Protection

SIRT1 and SIRT6 Signaling Pathways in Cardiovascular Disease Protection

Protective Role of Hydrogen Gas on Oxidative Damage and Apoptosis in Intestinal Porcine Epithelial Cells (IPEC-J2) Induced by Deoxynivalenol: A Preliminary Study

The Current State of NAD+‐Dependent Histone Deacetylases (Sirtuins) as Novel Therapeutic Targets

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The Current State of NAD⁺‐Dependent Histone Deacetylases (Sirtuins) as Novel Therapeutic Targets