Molecular identification and antibiotic resistance profiling of Salmonella species isolated from chickens in eastern Turkey

Abstract

Background: The aim of this study was to obtain quantitative data about the frequency, genotypic characterization and antibiotic resistance profiling of Salmonella agents in chicken flocks located in eastern Turkey. Results: Feces samples representing at least 20% of the flock area were collected via sock swabs from commercial poultry flocks in the study region in addition to internal organs (liver, spleen, intestine) collected at necropsy of suspected chickens belonging to small family enterprises. The samples were analyzed by conventional bacteriological methods (ISO 6579:2002/A1:2007) for isolation, and genus specific (invA) PCR for the identification of Salmonella spp. Then, two mPCR were set up to determine Salmonella serotypes and genotypic resistance status of the field isolates against ampicillin, tetracycline, trimethoprim-sulfamethoxazole and chloramphenicol antibiotics. In the PCR analysis of the suspected colonies, 98.5% were confirmed as Salmonella spp., and, the most prevalent serotype was identified as S. Infantis with the proportion of 26.6% (17/64), followed by S. Enteritidis with 21.9% (14/ 64) and S. Typhimurium with 9.4% (6/64). The findings related to antibiotic resistance genes revealed that the most frequently determined gene was sul1 with approximately 58%, while the blaTEM gene was detected at the lowest proportion with 20%, among Salmonella isolates. Conclusions: The results indicated that Salmonella infections constitute a potential risk for chicken flocks in the country and that genotypic resistance rates against various antibiotics should draw particular attention in terms of both human and animal health.

Virulence and antimicrobial resistance profile of non-typhoidal Salmonella enterica serovars recovered from poultry processing environments at wet markets in Dhaka, Bangladesh

“…Enteritidis (21.9%) and S . Typhimurium (9.4%) were isolated from chicken in Turkey [ 82 ]; Salmonella enterica serovars were identified in backyard poultry flocks in India [ 83 ]; S . Enteritidis and S .…”

Virulence and antimicrobial resistance profile of non-typhoidalSalmonella entericaserovars recovered from poultry processing environments at wet markets in Dhaka, Bangladesh

“…Likewise, streptomycin resistance gene (strA/B) was isolated in serovars of NTS with reliable prevalence rate. Arkali and Çetinkaya, (2020) detected 58% positive sul1 gene from the Salmonella isolates of chickens in eastern Turkey. In our study, harmonic and proportioned correlation were existent between genotypic and phenotypic resistance decoration.…”

“…The higher occurrence of S. Typhimurium in our study is synchronized with findings at home and abroad. Consecutively, S. Typhimurium and S. Enteritidis were isolated from raw chicken meat at retail markets in Malaysia (Thung et al, 2018); higher prevalence of S. Enteritidis (21.9%) and S. Typhimurium (9.4%) were isolated from chicken in Turkey (Arkali et al, 2020); Salmonella enterica serovars were identified in backyard poultry flocks in India (Samanta et al, 2014); S. Enteritidis and S. Typhimurium recovered from chicken meat in Egypt (Tarabees et al, 2017). Similarly, Suresh et al (2006) recovered S.…”

Virulence and Antimicrobial Resistance Profiles of Salmonella enterica Serovars Isolated from Chicken at Wet Markets in Dhaka, Bangladesh

“…In Malaysia, S. Typhimurium and S. Enteritidis were isolated from raw chicken meat at retail markets [70]. In Turkey, the prevalent serotype was identified as S. Enteritidis (21.9%) and S. Typhimurium (9.4%) from chicken [72]. In India, Salmonella enterica serovars were isolated from backyard poultry flocks [73].…”

“…Moreover, ESBL are usually encoded by large plasmids that are transferable from strain to strain and between bacterial species [96,100]. Arkali and Çetinkaya [72] detected sul1 gene with 58% among the Salmonella isolates from chickens in eastern Turkey. Consequently, Jahantigh et al [101] isolated the most prevalent tetA from broiler chicken in Iran.…”