Molecular identification and characterization of mannitol-negative methicillin-resistant Staphylococcus aureus

Shittu, Adebayo; Lin, Johnson; Morrison, Donald A.

doi:10.1016/j.diagmicrobio.2006.05.004

Cited by 15 publications

(8 citation statements)

References 24 publications

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“…The low specificity of mannitol (11%) for detecting S. aureus could be caused by the large number of coagulase-negative staphylococci (CoNS) isolates that were mannitol-positive. Mannitol-negative S. aureus strains have also been reported by other authors ( Kateete et al , 2010 ; Shittu et al , 2007 ). The identification of nine of these strains was correct based on the positivity of the coagulase and DNase tests.…”

supporting

confidence: 73%

“…Mannitol salt agar (MSA) is a conventional medium that is frequently used to screen nasal swab specimens for presumptive MRSA colonization based on the growth of yellow colonies derived from the fermentation of mannitol ( Chapman, 1945 ). However, clinical isolates of mannitol-negative MRSA have been widely reported ( Kateete et al , 2010 ; Shittu et al , 2007 ). The aim of this study was to describe the occurrence of S. aureus strains that do not ferment mannitol on MSA, which is an important screening method for the detection of MRSA colonization.…”

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confidence: 99%

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Mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swab specimens in Brazil

Santos¹,

Costa²,

Rabello³

et al. 2015

Braz. J. Microbiol.

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The isolation of mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swabs is reported. Among the 59 isolates, 9 (15%) isolates were mannitol-negative; all of these isolates were categorized as staphylococcal cassette chromosome mec (SCCmec) type IVa. This report emphasizes that mannitol fermentation on mannitol salt agar should not be used as the sole criterion when screening nasal swab specimens for S. aureus.

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supporting

confidence: 73%

mentioning

confidence: 99%

Mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swab specimens in Brazil

Santos¹,

Costa²,

Rabello³

et al. 2015

Braz. J. Microbiol.

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“…aureus in these media is based on hydrolysis of mannitol, and many microorganisms including coagulase negative staphylococci hydrolyze mannitol. In addition, mannitol negative MRSA strains have been reported [ 29 ]. We observed one strain that during primary-isolation was mannitol negative on ORSA and MSA.…”

Section: Discussionmentioning

confidence: 99%

Carriage and Genetic Diversity of Methicillin-Resistant Staphylococcus aureus among Patients and Healthcare Workers in a Serbian University Hospital

et al. 2015

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ObjectivesThere is a paucity of data on methicillin-resistant Staphylococcus aureus (MRSA) epidemiology among Balkan countries. The aim of our study was to determine the prevalence of nasal and pharyngeal carriages and diversity of MRSA among patients and healthcare workers (HCWs) in the major referral centre in Serbia, and to evaluate performance of three different media for MRSA screening.MethodsNasal and pharyngeal swabs were obtained from 195 patients and 105 HCWs in Emergency Department (ED), Surgical Department (SD) and Medical Department (MD). After broth enrichment, samples were inoculated onto MRSA-ID, ORSA and oxacillin-MSA and incubated for 24/48 hours. Characterisation of isolated MRSA strains was determined by MLVA, spa, SCCmec and agr typing, PVL genes detection and antimicrobial susceptibility.ResultsMRSA carriage prevalence was 11.8% in patients and 7.6% in HCWs. Introduction of pharyngeal swabs in screening procedure increased MRSA carriage rate by over 30%. Variable found to be independently associated with an increased risk for MRSA carriage was ED (odd ratio (OR) = 4.45, 95% confidence interval (CI) 1.78-11.14). A higher risk of multidrug-resistant MRSA carriage was observed among patients (OR = 22; 95% CI 1.92-251.54). CC5-MRSA-SCCmecI was the dominant clone among patients and HCWs in ED and MD, while high genetic diversity of community-associated MRSA (CA-MRSA) was shown in SD especially among HCWs. MRSA-ID was superior to the other tested media with a sensitivity/specificity of 95.2% and 99.6% after 48 hours of incubation.ConclusionsThese results indicate high MRSA carriage rate in the hospital and emergence of CA-MRSA through HCWs in these settings. MRSA-ID was the optimal available choice for MRSA screening.

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“…They pose a threat both to immunocompetent persons as well as to those whose immune system is impaired. Especially dangerous strains can be strains resistant to antibiotics, such as methicillin-resistant Staphylococcus aureus (MRSA) [23,34]. The presence of these bacteria were analysed in the case of the city park, a street (Ogrodowa St.), and the pharmacy.…”

Section: Discussionmentioning

confidence: 99%

The microbiological condition of public air in the city during autumn and winter

Adamus-Białek¹,

Filipiak²,

Wawszczak³

et al. 2019

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Introduction: Microorganisms live in most environments, and one of the most common is air. Bacteria, viruses, and fungi occur as bioaerosols, which allows them to spread. Airborne bacteria are potentially pathogenic, especially in areas densely populated by people. Aim of the research: To analyse the microbiological air pollution in different public places-busy streets, a city park, a cinema, and a pharmacy, in Kielce, during autumn and winter. Material and methods: The total number of bacteria was estimated based on the sedimentation method. The microbiological condition of air was compared to the standardised Polish norm. Additionally, the macroscopic (bacterial colony) and microscopic (Gram-stained bacterial cells) methods were used for bacterial differentiation. Results: Most of the air samples did not exhibit contamination, although the detected cases of significant microbiological pollution do pose a threat to public health. The air on the streets revealed the highest microbial contamination, and the lowest levels of microbes were found in the park. The influence of temperature and population density on the bacterial prevalence in the air has been proven. Despite low air temperatures, the amount of bacteria in most cases was close to the average pollution level. It was also observed that in the pharmacy, the glass separating customers and staff did not reduce the number of bacteria on either side. Conclusions: Special attention should be paid to places that can significantly influence the spread of pathogens due to large population (e.g. a cinema) or the presence of sick people (e.g. a pharmacy). Air should be monitored in such places. Streszczenie Wprowadzenie: Mikroorganizmy stwierdza się w prawie każdym środowisku, szczególnie powszechnie w powietrzu. Bakterie, wirusy i grzyby występują w postaci bioaerozoli, co pozwala im się przemieszczać. Bakterie przenoszone przez powietrze mogą należeć do patogennych grup, zwłaszcza w miejscach często uczęszczanych przez ludzi. Cel pracy: Analiza mikrobiologicznego zanieczyszczenia powietrza w różnych miejscach publicznych, takich jak ruchliwe ulice, park miejski, kino i apteka. Materiał i metody: Ogólną liczbę bakterii określono metodą sedymentacji. Stan mikrobiologiczny powietrza porównano z normą polską. Dodatkowo do różnicowania bakterii zastosowano metody makroskopowe (obserwacja kolonii bakterii) i mikroskopowe (obserwacja komórek bakterii wybarwionych metodą Grama). Wyniki: W większości próbek powietrza nie stwierdzono zanieczyszczenia, chociaż wykryte przypadki znacznego zanieczyszczenia mikrobiologicznego stanowią zagrożenie dla zdrowia publicznego. W powietrzu na ulicach odnotowano najwyższy poziom zanieczyszczenia mikrobiologicznego, najmniej drobnoustrojów występowało w parku. Dodatkowo wykazano wpływ temperatury i zaludnienia na występowanie bakterii w powietrzu. Mimo niskich temperatur powietrza liczba bakterii w większości przypadków była bliska średniego poziomu. Zaobserwowano również, że w aptece szyba dzieląca klientów i personel nie wpływa na zmni...

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Molecular identification and characterization of mannitol-negative methicillin-resistant Staphylococcus aureus

Cited by 15 publications

References 24 publications

Mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swab specimens in Brazil

Mannitol-negative methicillin-resistant Staphylococcus aureus from nasal swab specimens in Brazil

Carriage and Genetic Diversity of Methicillin-Resistant Staphylococcus aureus among Patients and Healthcare Workers in a Serbian University Hospital

The microbiological condition of public air in the city during autumn and winter

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