Molecular identification and characterization of Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh

Abstract

The aim of this study was to identify and characterize Salmonella species isolated from poultry value chains of Gazipur and Tangail districts of Bangladesh during the period of October 2015 to May 2016. For this purpose a total of 153 samples (35 chick meconium, 49 cloacal swab, 30 poultry carcass, 14 feed, 16 water, 7 transport swab, 2 floor swab) were collected and were subjected to various cultural, biochemical techniques and polymerase chain reaction (PCR). Furthermore, the isolated Salmonella species were characterized by antimicrobial susceptibility testing. Among the samples, 23.53% (n=36) were found to be associated with Salmonella species. The Salmonella species were identified by observing black centered colonies on XLD agar, positive to MR test and negative to VP and Indole test. All isolates of Salmonella species were positive to 16s rRNA gene based PCR (574 bp). Serogrouping of Salmonella species were performed by slide agglutination test using commercial Salmonella specific polyvalent O (A-I) antisera, Salmonella O group B (Factor O: 4, 5, 27) antisera and Salmonella O group D (Factor O: 9, 46) antisera. Among the 36 isolates, 30.56% (n=11) belonged to serogroup B and rest of the isolates 69.44% (n=25) to serogroup D. The most prevalent serogroup identified in this study was serogroup D. The isolated Salmonella species were subjected to antimicrobial susceptibility testing with the aid of disk diffusion method using 8 antimicrobial agents. All isolates of Salmonella species were susceptible to ciprofloxacin, norfloxacin, streptomycin and gentamicin. Out of 36 isolates 100% Salmonella species were resistant to erythromycin and tetracycline. The findings of this study revealed the presence of multidrug resistant Salmonella species in poultry value chains of Gazipur and Tangail districts of Bangladesh that posseses a serious threat to public and poultry health. To the best of our knowledge, this is the first report on the prevalence, serogrouping and antimicrobial resistance patterns of Salmonella species from poultry value chains of selected districts in Bangladesh.

Antimicrobial resistance pattern of Salmonella spp. isolated from poultry farms in Abakaliki, Nigeria

“…Fanissa et al (2022) reported a low prevalence of Salmonella species in Surabaya, Indonesia, with a frequency of 11.3%. Other researchers have observed a similar prevalence of Salmonella species; poultry samples 23.53% (Al-Mamun et al 2017); poultry production 37.9% (Mahmud et al 2011), chickens 11.54% (Abd El-Aziz et al 2021) and broiler farms 31.25% (Mridha et al 2020). Prevalence of Salmonella bacteria was detected in 21.3% of chickens analyzed (Telli et al 2022).…”

Virulence and antimicrobial resistance profile of non-typhoidalSalmonella entericaserovars recovered from poultry processing environments at wet markets in Dhaka, Bangladesh

“…Previously, the prevalence of Salmonella in Bangladesh was found 23.33% in poultry slaughter specimens (Momtaz et al, 2018); 26.6% in chicken cloacal swab, intestinal fluid, egg surface, hand wash, and soil of chicken market samples (Akond et al, 2012); 25.35% in the chicken cloacal swab, eggshells, intestinal contents, liver swabs, broiler meat, and swabs of slaughterhouse (Karim et al, 2017); 35% in broiler farms settings (Alam et al, 2020); 23.53% in poultry samples (Al Mamun et al, 2017); 37.9% in poultry production settings (Mahmud et al, 2011); 31.25% in broiler farm settings (Mridha et al, 2020); 42% in broiler chicken (Sarker et al, 2021) and 65% in frozen chicken meat (Parvin et al, 2020). Successively, Siddiky et al (2021) found the prevalence of Salmonella 8.62% in broiler, 6.89% in sonali and 3.1% in native chicken cecal contents.…”