Molecular identification and differential proteomics of drug resistant Salmonella Typhi

Safi, Aziz Ur Rehman; Bendixen, Emøke; Rahman, Hazir; Khattak, Baharullah; Wu, Wei; Ullah, Waheed; Khan, Nasar; Ali, Farhad; Yasin, Nusrat; Qasim, Muhammad

doi:10.1016/j.diagmicrobio.2022.115883

Cited by 4 publications

(1 citation statement)

References 35 publications

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“…Meanwhile, the overexpression of OmpX in E. aerogenes results in higher β-lactam resistance, which can be explained by a significant decrease in Omp36 porin [ 111 ]. OmpX was found to be 1.7 times more abundant in drug-resistant S. typhimurium isolates than in drug-sensitive isolates [ 112 ]. These findings imply that the under or overexpression of ompX affects hydrophobic chemical transport across the membrane but does not impact substrate preference [ 80 ].…”

Section: Ompxmentioning

confidence: 99%

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Zhou

Wang

et al. 2023

Microorganisms

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Gram-negative bacteria depend on their cell membranes for survival and environmental adaptation. They contain two membranes, one of which is the outer membrane (OM), which is home to several different outer membrane proteins (Omps). One class of important Omps is porins, which mediate the inflow of nutrients and several antimicrobial drugs. The microorganism’s sensitivity to antibiotics, which are predominantly targeted at internal sites, is greatly influenced by the permeability characteristics of porins. In this review, the properties and interactions of five common porins, OmpA, OmpC, OmpF, OmpW, and OmpX, in connection to porin-mediated permeability are outlined. Meanwhile, this review also highlighted the discovered regulatory characteristics and identified molecular mechanisms in antibiotic penetration through porins. Taken together, uncovering porins’ functional properties will pave the way to investigate effective agents or approaches that use porins as targets to get rid of resistant gram-negative bacteria.

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Section: Ompxmentioning

confidence: 99%

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Zhou

Wang

et al. 2023

Microorganisms

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Understanding metabolic resistance strategy of clinically isolated antibiotic-resistant bacteria by proteomic approach

Peng,

Li,

Peng

2024

Expert Review of Proteomics

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Metabolic Proteins Expression Up-Regulated in Blood-Borne Extensively Drug-Resistant Salmonella Typhi Isolates from Pakistan

Yasin,

Rahman,

Qasim

et al. 2024

Medicina

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Background and Objectives: In the undertaken study, proteomics alterations of blood-borne XDR S. Typhi isolated from Pakistan were investigated using mass spectrometry. Materials and Methods: MDR and XDR S. Typhi total protein lysates were fractionated, digested, and processed for nanoflow LC-LTQ-Orbitrap MS analysis. Results: Among the 1267 identified proteins, 37 were differentially regulated, of which 28 were up-regulated, and 9 were down-regulated in XDR S. Typhi as compared to MDR S. Typhi. Based on the functional annotation, proteins found up-regulated are involved mainly in metabolic pathways (ManA, FadB, DacC, GpmA, AphA, PfkB, TalA, FbaB, OtsA, 16504242), the biosynthesis of secondary metabolites (ManA, FadB, GlpB, GpmA, PfkB, TalA, FbaB, OtsA), microbial metabolism in diverse environments (FadB, GpmA, PfkB, NfnB, TalA, FbaB), and ABC transporters (PstS, YbeJ, MglB, RbsB, ArtJ). Proteins found down-regulated are involved mainly in carbon metabolism (FadB, GpmA, PfkB, FalA, FbaB) and the biosynthesis of amino acids (GpmA, PfkB, TalA, FbaB). Most of the identified differential proteins were predicted to be antigenic, and matched with resistome data. Conclusions: A total of 28 proteins were up-regulated, and 9 were down-regulated in XDR S. Typhi. Further characterization of the identified proteins will help in understanding the molecular signaling involved in the emergence of XDR S. Typhi.

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Molecular identification and differential proteomics of drug resistant Salmonella Typhi

Cited by 4 publications

References 35 publications

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Outer Membrane Porins Contribute to Antimicrobial Resistance in Gram-Negative Bacteria

Understanding metabolic resistance strategy of clinically isolated antibiotic-resistant bacteria by proteomic approach

Metabolic Proteins Expression Up-Regulated in Blood-Borne Extensively Drug-Resistant Salmonella Typhi Isolates from Pakistan

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