Molecular identification and evaluation of Coxiella-like endosymbionts genetic diversity carried by cattle ticks in Algeria

Rahal, Mohamed; Medkour, Hacène; Diarra, Adama Zan; Bitam, Idir; Parola, Philippe; Mediannikov, Oleg

doi:10.1016/j.ttbdis.2020.101493

Cited by 16 publications

(9 citation statements)

References 26 publications

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“…This was not surprising, as it has been documented that the prevalence of these bacteria varies widely in various species of ticks. This prevalence was in accordance with studies by Arthan et al [ 52 ] and Rahal et al [ 53 ], who reported prevalences of 42.2% and 51.7% in ticks from vegetation and cattle, respectively. Taking this into consideration, the potential of these CLEs to adapt to vertebrate hosts and evolve into pathogenic Coxiella cannot be ruled out.…”

Section: Discussionsupporting

confidence: 93%

Molecular Detection of Tick-Borne Bacteria from Amblyomma (Acari: Ixodidae) Ticks Collected from Reptiles in South Africa

2022

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Reptiles are hosts for various tick species and tick-associated organisms, many of which are zoonotic. However, little is known about the presence and diversity of tick-borne bacteria infecting reptiles and their ticks in South Africa. Amblyomma ticks (n = 253) collected from reptiles were screened for the presence of Coxiella, Anaplasma, Rickettsia, and Borrelia species by amplification, sequencing and phylogenetic analysis of the 16S rRNA, 23S rRNA, gltA, OmpA, and Flagellin genes, respectively. This study recorded the presence of reptile associated Borrelia species and Coxiella-like endosymbiont in South Africa for the first time. Furthermore, a spotted fever group Rickettsia species was observed in 7 Amblyomma marmoreum and 14 Amblyomma sylvaticum from tortoises of genera Kinixys and Chersina. Francisella-like endosymbiont was observed from 2 Amblyomma latum collected from the Mozambique spitting cobra, Naja mossambica. Coxiella burnetii and Anaplasma spp., were not detected from the current samples. Although the direct evidence that reptiles can act as reservoir hosts remains to be determined, observations from this study provide indications that reptilian ticks may play a role in the transmission of pathogenic bacteria to homothermic animals. Furthermore, the absence of Anaplasma spp., and C. burnetii does not mean that these pathogens should be completely neglected.

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Section: Discussionsupporting

confidence: 93%

Molecular Detection of Tick-Borne Bacteria from Amblyomma (Acari: Ixodidae) Ticks Collected from Reptiles in South Africa

2022

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“…is known as an endosymbiont essential for the survival and reproduction of hard ticks [ 26 , 31 ]. Coxiella -like bacteria are a large group of yet-to-be-isolated and characterized bacteria phylogenetically close to the causative agent of Q fever, C. burnetii [ 32 ]. These Coxiella -like bacteria are maternally inherited endosymbionts, highly prevalent in tick populations, and engaged in mutualistic interactions with their arthropod hosts [ 33 ].…”

Section: Discussionmentioning

confidence: 99%

Microbiome of Haemaphysalis longicornis Tick in Korea

Kim

et al. 2021

Korean J Parasitol

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Ticks can transmit pathogenic bacteria, protozoa, and viruses to humans and animals. In this study, we investigated the microbiomes of Haemaphysalis longicornis according to sex and life stages. The Shannon index was significantly higher for nymphs than adult ticks. Principal coordinates analysis showed that the microbiome composition of female adult and male adult ticks were different. Notably, Coxiella-like bacterium (AB001519), known as a tick symbiont, was found in all nymphs and female adult ticks, but only one out of 4 male adult ticks had Coxiella-like bacterium (AB001519). In addition, Rickettsia rickettsii, Coxiella burnetii, and Anaplasma bovis were detected in this study.

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“…Studies about localization of CLEs in O. maritimus and D. marginatus tissues identified, thanks to fluorescence in situ hybridization, highlighted the tropism of CLEs for ovaries and Malpighian tubules, suggesting their possible role in the vertical transmission and nutritional symbiosis [8]. The possible horizontal transmission between ticks of CLEs via the vertebrate host has been also recently proposed by Rahal et al [37] that indicate that Coxiella-like bacteria are not only tick host-associated. CLEs have been recently described in domestic animals [41] and the bacteria have been reported as cause of lethal systematic infections in domestic birds.…”

Section: Discussionmentioning

confidence: 79%

“…For years, C. burnetii has been considered the only species of the Coxiella genus. The most recent studies, mainly on 16S rRNA gene sequencing, highlighted the presence of novel Coxiella-like organisms, a family of bacteria that act as commensal and mutualistic symbionts of several tick species [5,15,21,37,44,47,49]. Coxiella-like endosymbionts (CLEs) have high homology with the pathogen C. burnetii by grouping in a monophyletic clade based on 16S rRNA sequence phylogenetic analyses and show a reduced genome as compared to that of C. burnetii [20,21,43].…”

Section: Introductionmentioning

confidence: 99%

Coxiellaceae in Ticks from Human, Domestic and Wild Hosts from Sardinia, Italy: High Diversity of Coxiella-like Endosymbionts

et al. 2021

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Purpose Coxiella burnetii is known for its potential as veterinary and human bacterial pathogen. The bacteria have been described in ticks, but their role in transmission of Q fever in humans is considered low. Coxiella endosymbionts closely related to C. burnetii have been also isolated from an extensive range of tick species and evidence is growing that these endosymbionts could be linked to human bacteremia. The aim of this study was to get new information on the presence of Coxiella species in ticks infesting wild and domestic hosts in Sardinia, Italy. Methods Here, 138 ticks collected from the study area were analyzed for the presence of C. burnetii and Coxiella-like bacteria by polymerase chain reaction (PCR), sequencing and philogenetic analyses using a set of primers targeting the 16S rRNA gene. Results DNA of Coxiella species was detected in 69% of the total ticks examined. Based on phylogenetic analysis, the 16S rRNA Coxiella genotypes identified in this study grouped in strongly supported monophyletic clades with identified reference sequences of CLEs detected from Rhipicephalus, Dermacentor, Haemaphysalis and Ornithodoros species and with Coxiella burnetii strains isolated worldwide. Conclusion This study reports the molecular detection of a high diversity of Coxiella-like bacteria in Sardinian ticks and confirms also the presence of C. burnetii in tick species previously identified in the island. The role that Coxiella-like endosymbionts play in Sardinian ticks and in their vertebrate hosts needs to be explored further.

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Molecular identification and evaluation of Coxiella-like endosymbionts genetic diversity carried by cattle ticks in Algeria

Cited by 16 publications

References 26 publications

Molecular Detection of Tick-Borne Bacteria from Amblyomma (Acari: Ixodidae) Ticks Collected from Reptiles in South Africa

Molecular Detection of Tick-Borne Bacteria from Amblyomma (Acari: Ixodidae) Ticks Collected from Reptiles in South Africa

Microbiome of Haemaphysalis longicornis Tick in Korea

Coxiellaceae in Ticks from Human, Domestic and Wild Hosts from Sardinia, Italy: High Diversity of Coxiella-like Endosymbionts

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