Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene

Abstract

Abstract. Tambunan VB, Apriyanto A, Ajambang W, Etta CE, Sahari B, Buchori D, Hidayat P. 2020. Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene. Biodiversitas 21: 3263-3270. Oil palm pollinating weevil Elaeidobius kamerunicus is a very important insect pollinator in oil palm plantation. However, there is still lack of information about molecular identification and population genetic study in this species. The purpose of this study was to explore the effectiveness of oil palm pollinating weevil identification using mitochondrial DNA of COI gene and to assess its genetic variation between different locations and countries. We sequenced the DNA barcode of 36 individuals of this species using the mtDNA Cytochrome Oxidase I (COI) gene to explore their genetic variation, identity and phylogenetic relationship. The COI gene sequences generated from this study were successful in identifying E. kamerunicus. Phylogenetic analysis also revealed 3 well-supported monophyletic haplogroups of E. kamerunicus population. In addition, genetic differentiation analysis revealed that most populations from Indonesia were different from Malaysian and Cameroonian populations indicating that there was a genetic variation between the population samples from these countries. The overall E. kamerunicus used in this study were geographically structured in two regions; outside Indonesia region (Cameroon and Malaysia) and Indonesia region. These results demonstrate the feasibility of using COI gene sequence for molecular identification and population genetic study of E. kamerunicus species.

Genetic Diversity and Population Structure in Elaeidobius kamerunicus (Coleoptera: Curculionidae) Inferred from mtDNA COI and Microsatellite Markers

“…It is clear that the haplotype form one site or island was not originated from one haplotype. Therefore, this result supports previous results that the genetic population between islands is not isolated by geographic [44,45].…”

Draft genome sequence, annotation, and SSR mining data of Elaeidobius kamerunicus Faust., an essential oil palm pollinating weevil

“…The introduction of this weevil species into oil palm plantations successfully improved fruit set, increased the yield of oil palm, and reducing the need for assisted pollination [2] . Recent studies of this species have only focused on analyzing genetic diversity and species identification [1] , [3] , [4] . Interestingly, several divergent mitochondrial lineages in this species have been discovered based on the information of cytochrome c oxidase subunit I (COI) and cytochrome c oxidase subunit II (COII) gene sequences [1] , [3] .…”

“…Recent studies of this species have only focused on analyzing genetic diversity and species identification [1] , [3] , [4] . Interestingly, several divergent mitochondrial lineages in this species have been discovered based on the information of cytochrome c oxidase subunit I (COI) and cytochrome c oxidase subunit II (COII) gene sequences [1] , [3] . Our recent study successfully obtained the complete mitochondrial genome of E.kamerunicus from the partial dataset from this report, representing the first complete mitogenome for this species [5] .…”

The complete mitochondrial genome of oil palm pollinating weevil, Elaeidobius kamerunicus Faust. (Coleoptera : Curculionidae)

“… 2020 ; Tambunan et al. 2020 ). Nevertheless, the complete mitochondrial genome information of this species has never been reported before.…”

“…Introduction of the weevil into oil palm plantations successfully improved fruit set and increased the yield of oil palm (Li et al 2019). Recently, several genetic diversity studies of this species have been conducted using either nuclear or mitochondrial genetic information (Bakara et al 2019(Bakara et al , 2020Haran et al 2020;Tambunan et al 2020). Nevertheless, the complete mitochondrial genome information of this species has never been reported before.…”