Molecular identification and relatedness of potato witches’-broom phytoplasma isolates from four potato cultivars

Khadhair, A. H.; Hiruki, C.; Hwang, Shue Fang; Wang, Keri

doi:10.1016/s0944-5013(97)80041-9

Cited by 25 publications

(15 citation statements)

References 13 publications

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“…1. The expected sizes, based on analysis of the putative restriction sites, were in agreement with the fragment sizes obtained by enzymic RFLP analysis of the amplified 16S rRNA gene (Lee et al, 1993(Lee et al, , 1998Khadhair et al, 1997b;Jomantiene et al, 1998;Wang et al, 1998;Wang & Hiruki, 2001;Jacobs et al, 2003). Analysis of putative restriction sites of the partial 16S rRNA gene revealed that these phytoplasmas share very similar or identical restriction profiles, whereas the BLL, FM, ILEY1 and ILEY2 phytoplasmas were distinguished from the CP R phytoplasma by the absence of the HhaI site at position 1089, AluI site at position 993, HhaI site at position 234 and MseI site at position 944 in the 16S rRNA gene sequence of the CP R phytoplasma.…”

Section: Putative Restriction Sites In the 16s Rrna Genesupporting

confidence: 84%

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

Hiruki

Wang

2004

International Journal of Systematic and Evolutionary Microbiology

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Clover proliferation phytoplasma (CP R ) is designated as the reference strain for the CP phylogenetic group or subclade, on the basis of molecular analyses of genomic DNA, the 16S rRNA gene and the 16S-23S spacer region. Other strains related to CP R include alfalfa witches'-broom (AWB), brinjal little leaf (BLL), beet leafhopper-transmitted virescence (BLTV), Illinois elm yellows (ILEY), potato witches'-broom (PWB), potato yellows (PY), tomato big bud in California (TBBc) and phytoplasmas from Fragaria multicipita (FM). Phylogenetic analysis of the 16S rRNA gene sequences of BLL, CP R , FM and ILEY, together with sequences from 16 other phytoplasmas that belong to the ash yellows (AshY), jujube witches'-broom (JWB) and elm yellows (EY) groups that were available in GenBank, produced a tree on which these phytoplasmas clearly clustered as a discrete group. Three subgroups have been classified on the basis of sequence homology and the collective RFLP patterns of amplified 16S rRNA genes. AWB, BLTV, PWB and TBBc are assigned to taxonomic subgroup CP-A, FM belongs to subgroup CP-B and BLL and ILEY are assigned to subgroup CP-C. Genetic heterogeneity between different isolates of AWB, CP R and PWB has been observed from heteroduplex mobility assay analysis of amplified 16S rRNA genes and the 16S-23S spacer region. Two unique signature sequences that can be utilized to distinguish the CP group from others were present. On the basis of unique properties of the DNA from clover proliferation phytoplasma, the name 'Candidatus Phytoplasma trifolii' is proposed for the CP group.

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Section: Putative Restriction Sites In the 16s Rrna Genesupporting

confidence: 84%

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

Hiruki

Wang

2004

International Journal of Systematic and Evolutionary Microbiology

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“…However, other phytoplasmas have recently been reported on potato in central and eastern Europe (Paltrinieri and Bertaccini 2007;Bogoutdinov et al 2008;Girsova et al 2008). The potato witches'-broom disease occurs in Europe, Asia, and North America, and is usually of minor economic importance (Brcak et al 1969;Harrison and Roberts 1969;Maramorosch et al 1970;Hodgson et al 1974;Rich 1983;Khadhair et al 1997;2003;Slack 2001).…”

Section: Introductionmentioning

confidence: 99%

Survey and molecular detection of phytoplasmas associated with potato in Romania and southern Russia

Ember¹,

Ács²,

Munyaneza³

et al. 2011

Eur J Plant Pathol

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In recent years, emerging phytoplasma diseases of potato (Solanum tuberosum L.) have increasingly become important in central and eastern Europe. Accurate identification of phytoplasmas and their insect vectors is essential to developing effective management strategies for diseases caused by these plant pathogens. Potato phytoplasma diseases in Europe were for a long time diagnosed only on the basis of visual symptoms. However, this approach is not very reliable and the use of modern molecular techniques such as polymerase chain reaction (PCR) is required in order to accurately determine the etiology of these phytoplasma diseases. A survey and identification of phytoplasmas associated with potato crops in Romania and southern Russia were conducted based on modern molecular techniques. Symptomatic potato plants were collected from several fields and tested for phytoplasmas by PCR.Also, selected crops and weeds in the vicinity of these potato fields were sampled and tested for phytoplasmas. Stolbur ("Candidatus Phytoplasma solani"; 16SrXII-A) was the only phytoplasma detected in potato and adjacent crops, including tomato (Solanum lycopersicum), pepper (Capsicum annuum), eggplant (Solanum melongena), and beet (Beta vulgaris). This phytoplasma was also detected in weeds, particularly Convolvulus arvensis, Cuscuta sp., and Euphorbia falcata. Genotyping of obtained stolbur isolates on tuf genes revealed that they all had the same RFLP profile corresponding to the tuf-type 'b' (VK Type II). Stolbur-affected potato plants produced a large number of spongy tubers that resulted in commercially unacceptable potato chips upon processing.

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“…Nucleic acid-based assays' high sensitivity and PCR assays using Phytoplasma-specific "universal primers", designed from 16S rRNA gene or other conserved sequences, are useful tools for diagnosis and confirmation of phytoplasmal infections. Identification of putative Phytoplasma can be achieved by RFLP analysis of PCR products (Khadhair et al 1997). Nested PCR may be performed by using two universal primer pair or using one universal primer pair followed by specific primer pair (Faggioli et al 2004).…”

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confidence: 99%

“…Phytoplasma differ in their 16S rDNA sequence by not more than 14%, whereas their respective 16-23S differed by as much as 22%. This added variation may contribute to the improved accuracy of this method to sequence Phytoplasma classification at the subgroup level (Khadhair et al 1997). Jordan is recognised as a developing agricultural country in the Mediterranean region having different ecological habitats, different cultivated crops as well as forestry showing the symptoms of Phytoplasma infection.…”

Section: Please Scroll Down For Articlementioning

confidence: 99%

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Detection ofPhytoplasmafrom deferent infected hosts in Jordan based on PCR amplification of the 16S rDNA sequences

Al-Momani

Al‐Muaikel

2014

Archives Of Phytopathology And Plant Protection

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Sixteen leaf samples, both healthy and Phytoplasma diseased, were collected from different plants such as grape, peach, almond, tomato, paper, squash, apple and pear in northern Jordan. Extracted DNA from diseased grape, peach, almond, tomato, paper and squash plus from infected periwinkle (Catharanthus roseus) samples were amplified with the Phytoplasma universal 16S rDNA sequences primer pairs. Extracted DNA samples from healthy and diseased apple and pear plants were not amplified with the same primer pairs. All the PCR-amplified DNA samples show a common band with size of 558 bp, indicating Phytoplasma pathogens as a diseasecausative agent for grape, peach, almond, tomato, paper and squash plants. The restriction fragment length polymorphisms of Alu1 enzyme for the amplified 16S rDNA sequences shows the same DNA fragment patterns indicating no or a limited diversity among the DNA of the detected Phytoplasma pathogens.

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Molecular identification and relatedness of potato witches’-broom phytoplasma isolates from four potato cultivars

Cited by 25 publications

References 13 publications

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

Clover proliferation phytoplasma: ‘Candidatus Phytoplasma trifolii’

Survey and molecular detection of phytoplasmas associated with potato in Romania and southern Russia

Detection ofPhytoplasmafrom deferent infected hosts in Jordan based on PCR amplification of the 16S rDNA sequences

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