Molecular Identification and Serogrouping of <i>Pasteurella mutocida</i> Field Isolats

Pujiono, Agustin Eka; Wibawan, I Wayan Teguh; Afiff, Usamah; Setiyaningsih, Surachmi

doi:10.1088/1755-1315/197/1/012046

Cited by 3 publications

(3 citation statements)

References 9 publications

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“…The problem with the limitation of sample size in this study also be found as previously reported by other researchers in Indonesia. Pujiono et al [27] just used three P. multocida isolates to identify and serogroup of P. multocida field isolates. In their study, they were combined the use of 16S rRNA test with other specific primers such as the primers to amplify the kmt gene and bcbD gene.…”

Section: Discussionmentioning

confidence: 99%

Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

2020

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Aim: This study aimed to determine the molecular characteristics of Pasteurella multocida isolates originated from Sumba Island, East Nusa Tenggara Province. Materials and Methods: The isolates of P. multocida stored in frozen storage were cultured in blood agar as a selective medium and identified conventionally. Molecular tests were initiated by DNA isolation and then followed by polymerase chain reaction tests with specific primers for the determination of P. multocida serotype A or B. Positive strain of serotype B was then confirmed molecularly using 16S rRNA gene primer and followed by the sequencing of nucleotides. Results: The study showed that both P. multocida isolates from Sumba island, i.e. PM1 is isolated from East Sumba district, while PM2 isolated from West Sumba district have 99.6% homology. Both isolates also known have 99% similarities with P. multocida originated from India, Britain, and Japan, respectively. The isolates share the same clade in the phylogenetic tree. Conclusion: The 16S rRNA sequencing revealed a high similarity of P. multocida serotype B:2 isolated from Sumba island with the Indian isolates although the sample size is very small. Therefore, further molecular studies like multilocus sequence typing, VNTR need to be performed using a larger number of samples to establish the genetic relatedness observed in this study.

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Section: Discussionmentioning

confidence: 99%

Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

2020

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“…The isolates were collected from cases of fowl cholera in poultry in 5 provinces of Korea between 2014 and 2019, and the host, age, clinical signs, macroscopic lesions and the mortality rate for each case are shown in Table 1. The isolates were cultured on blood agar (Synergy Innovation, Seongnam, Korea) and MacConkey agar (Becton Dickinson, Sparks, MD, USA) in the air at 37°C for 24 h. Suspected colonies (mucoid and non-haemolytic) were identified by Gram staining and biochemical identification using the VITEK II system (BioMérieux, Marcy l'Etoile, France) according to the previous methods (Pujiono et al, 2018). For further confirmation, PCR assay was performed with primers specific for the amplification of the KMT1 gene (Townsend et al, 1998).…”

Section: Bacterial Isolates and Dna Extractionmentioning

confidence: 99%

Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea

Jeong

Kang

Jeong

et al. 2021

Braz. J. Poult. Sci.

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Pasteurella multocida causes fowl cholera which is an economically important disease in poultry industries around the world. In this study, we analyzed the capsular genotype, lipopolysaccharide (LPS) genotype, virulence-associated genes (VAGs) patterns, antimicrobial resistance and genetic diversity in a total of 9 P. multocida isolates from poultry with fowl cholera between 2014 and 2019 in Korea. When combining the capsular types with the LPS genotypes, two isolates of the 9 isolates were A:L3, and the others were non-typeable (NT): L3. Of the 23 VAGs, all the isolates harbored ptfA, fimA, hsf-1, pfhA, exbB, exbD, tonB, hgbA, hgbB, fur, sodA, sodC, pmHAS, ompA, ompH, oma87, plpB, psl, and nanH, whereas toxA gene was not detected in any of the 9 isolates. In addition, among the 11 antimicrobials, most of the isolates except for one isolate resistant to florfenicol, exhibited susceptibility to all the antimicrobials. Multi-locus sequence typing (MLST) analysis revealed 5 different sequence types (ST): ST8, ST351, ST352, ST353, and ST368. The ST351, ST352, ST353, and ST368 were identified for the first time in this study, and ST352 and ST353 isolates were largely prevalent nationwide. These STs isolates should be monitored continuously because in some cases, ST352 and ST353 isolates demonstrated high mortality rates. Although only limited numbers of isolates have been analyzed, our findings provide overall characteristics and epidemiological information of the P. multocida strains recently prevalent in Korea.

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“…ough distinction of Pasteurella from related genera, and typically distinction of P. multocida from non-multocida Pasteurella species,was feasible prior to the advent of these methods (papers which did not adequately categorize their infectious agents as definitively belonging to the Pasteurella genus having been excluded from our analysis), advancements have shown that even with current molecular methods, proper identification of non-multocida species remains challenging [2][3][4][5][6][7][8][9][10].…”

Section: Literature Reviewmentioning

confidence: 99%

Pasteurella Endocarditis: A Case Report and Statistical Analysis of the Literature

Porter

Hay

2020

Case Reports in Infectious Diseases

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Pasteurella is a genus of commensal bacteria of the oral cavity of several domesticated animals and a common cause of cellulitis after animal bites. Pasteurella has also been reported as a rare cause of endocarditis, with only 35 prior cases of definite Pasteurella endocarditis in the literature. Here, we present a case of Pasteurella multocida endocarditis treated successfully with surgery and antibiosis, as well as a review of the literature with statistical analysis of correlations between risk factors and clinical outcomes, as well as between treatment choices and clinical outcomes. Despite the small sample size, our analysis indicates a statistically significant correlation between comorbid liver disease and mortality, as well as a significant negative correlation between surgical treatment and mortality. This analysis implies a need for surgical management of endocarditis due to Pasteurella species and for more aggressive management of Pasteurella endocarditis in the setting of comorbid liver disease.

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Molecular Identification and Serogrouping of Pasteurella mutocida Field Isolats

Cited by 3 publications

References 9 publications

Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

Molecular characteristic of Pasteurella multocida isolates from Sumba Island at East Nusa Tenggara Province, Indonesia

Investigation of Genetic Diversity of Pasteurella multocida Isolated from Diseased Poultry in Korea

Pasteurella Endocarditis: A Case Report and Statistical Analysis of the Literature

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