Molecular Identification and Ultrastructural and Phylogenetic Studies of Cyanobacteria from Association with the White Sea Hydroid<i>Dynamena pumila</i>(L., 1758)

Кокшарова, О. А.; Kravzova, T. R.; Lazebnaya, I. V.; Gorelova, Olga; Baulina, O. I.; Lazebny, O. E.; Fedorenko, T. A.; Лобакова, Е. С.

doi:10.1155/2013/760681

Cited by 4 publications

(1 citation statement)

References 28 publications

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“…For molecular typing cyanobacterial genomic DNA was isolated according to [ 20 ] and synthetic oligonucleotides (“Synthol,” Moscow, Russia) have been used as cyanobacterial primers for 16S–23S rRNA PCR, according to [ 21 ]. As a second molecular marker the nifH gene has been used with corresponding PCR primers [ 22 ].…”

Section: Methodsmentioning

confidence: 99%

Biochemical and Molecular Phylogenetic Study of Agriculturally Useful Association of a Nitrogen-Fixing Cyanobacterium and NoduleSinorhizobiumwithMedicago sativaL.

Karaushu

Lazebnaya

Kravzova

et al. 2015

BioMed Research International

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Seed inoculation with bacterial consortium was found to increase legume yield, providing a higher growth than the standard nitrogen treatment methods. Alfalfa plants were inoculated by mono- and binary compositions of nitrogen-fixing microorganisms. Their physiological and biochemical properties were estimated. Inoculation by microbial consortium of Sinorhizobium meliloti T17 together with a new cyanobacterial isolate Nostoc PTV was more efficient than the single-rhizobium strain inoculation. This treatment provides an intensification of the processes of biological nitrogen fixation by rhizobia bacteria in the root nodules and an intensification of plant photosynthesis. Inoculation by bacterial consortium stimulates growth of plant mass and rhizogenesis and leads to increased productivity of alfalfa and to improving the amino acid composition of plant leaves. The full nucleotide sequence of the rRNA gene cluster and partial sequence of the dinitrogenase reductase (nifH) gene of Nostoc PTV were deposited to GenBank (JQ259185.1, JQ259186.1). Comparison of these gene sequences of Nostoc PTV with all sequences present at the GenBank shows that this cyanobacterial strain does not have 100% identity with any organisms investigated previously. Phylogenetic analysis showed that this cyanobacterium clustered with high credibility values with Nostoc muscorum.

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Section: Methodsmentioning

confidence: 99%

Biochemical and Molecular Phylogenetic Study of Agriculturally Useful Association of a Nitrogen-Fixing Cyanobacterium and NoduleSinorhizobiumwithMedicago sativaL.

Karaushu

Lazebnaya

Kravzova

et al. 2015

BioMed Research International

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Similarity and diversity of the Desmodesmus spp. microalgae isolated from associations with White Sea invertebrates

et al. 2014

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Similarity and diversity of the phenotype and nucleotide sequences of certain genome loci among the single-celled microalgae isolated from White Sea benthic invertebrates were studied to extend the knowledge of oxygenic photoautotrophs forming microbial communities associated with animals. We compared four Desmodesmus isolates (1Hp86E-2, 1Pm66B, 3Dp86E-1, 2Cl66E) from the sponge Halichondria panicea, trochophore larvae of the polychaete Phyllodoce maculata, and the hydroids Dynamena pumila and Coryne lovenii, respectively. The microalgae appeared to be very similar featuring the phenotypic and genetic traits characteristics of unicellular representatives of the genus Desmodesmus. At the same time, isolates from different animal species displayed certain differences in (i) the epistructure morphology; (ii) type and number of the inclusions such as interthylakoid starch grains and cytoplasmic oil bodies and (iii) fatty acid composition; in Desmodesmus sp. 1Hp86E-2, these differences were most pronounced. Phylogenetic analysis based on ITS1-5.8S rRNA-ITS2 and rbcL sequences showed that all isolates studied differ from known classified representatives of Desmodesmus combining a deletion in the conservative 5.8S rRNA gene and long AC-microsatellite repeats in the ITS1 whereas 1Hp86E-2 represented a distinct branch within this group.

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Nitrogen availability modulates CO2 tolerance in a symbiotic chlorophyte

et al. 2016

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Molecular Identification and Ultrastructural and Phylogenetic Studies of Cyanobacteria from Association with the White Sea HydroidDynamena pumila(L., 1758)

Cited by 4 publications

References 28 publications

Biochemical and Molecular Phylogenetic Study of Agriculturally Useful Association of a Nitrogen-Fixing Cyanobacterium and NoduleSinorhizobiumwithMedicago sativaL.

Biochemical and Molecular Phylogenetic Study of Agriculturally Useful Association of a Nitrogen-Fixing Cyanobacterium and NoduleSinorhizobiumwithMedicago sativaL.

Similarity and diversity of the Desmodesmus spp. microalgae isolated from associations with White Sea invertebrates

Nitrogen availability modulates CO2 tolerance in a symbiotic chlorophyte

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