Molecular identification of a new isolate of Cucumber mosaic virus subgroup II from basil (Ocimum sanctum) in India

Khan, Asif Ali; Sharma, Rajesh; Afreen, Bushra; Naqvi, Q. A.; Kumar, Susheel; Snehi, S. K.; Raj, S. K.

doi:10.1007/s12600-011-0146-8

Cited by 9 publications

(5 citation statements)

References 8 publications

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“…CMV is known to be infecting several crops, weeds and medicinal plants worldwide. In India natural occurrence of CMV has been reported on a range of crops such as Lily [43], Datura [45], Banana [18,53], Tulasi [17] Black pepper, Betel vine [14], Brinjal [24], Bottle gourd, Snake gourd [32], Carrot [2], Castor [39], Chilli, Chrysanthemum [46], Cucumber [26], Gerbera [11,50], Geranium [52], Gladiolus [8], Jatropha [38], Ornithogalum [51], Petunia [10] and Tomato [12,23,37,47] are characterized. The present investigation reports the natural occurrence of CMV on coleus and its identification based on Electron microscopy, ELISA testing and complete genome sequencing.…”

Section: Discussionmentioning

confidence: 99%

Characterization of cucumber mosaic virus infecting coleus (Plectranthus barbatus) in Karnataka

et al. 2019

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Plectranthus barbatus also known by the synonym Coleus forskohlii it is called as forskohlii and Indian coleus. It is a tropical perennial herb belongs to the family Lamiaceae widely cultivated in India used as traditional medicinal crop. Its tuberous roots produce forskolin, an extract useful for pharmaceutical preparations and research in cell biology. The incidence of mosaic with dark and light green patches, mottling, leaf distortion and reduction growth was noticed in commercial cultivation of coleus. For identification of the virus, the infected leaf sample extract was mechanically inoculated to different hosts such as chilli, tobacco, tomato, cucumber, cowpea and Chenopodium amaranticolor. Host range studies revealed that the virus showed severe mosaic symptoms on Nicotiana spp. and Cucumis spp. The virus produced systemic and local lesion symptoms in a different host. The Leaf dip preparation of virus infected leaf extract was observed under an electron microscope showed the presence of isometric particles of 28 nm in size. The healthy and infected samples were tested using DAC-ELISA against antibodies of CMV, GBNV and TSV the infected samples showed strong positive reaction with 1.85 optical density to CMV antibodies indicated the presence of CMV. For molecular identification, total RNA was isolated and used for RT-PCR amplification using CMV specific primers. RT-PCR resulted in the positive amplification in virus infected samples but not from a healthy control. The complete genome of CMV RNA-1 consists of 3360 nucleotides (nt) encoding replicase gene of 807 amino acids (aa). The CMV RNA-2 was 2983 nt in length containing 2a (859 aa) encoding RNA dependent RNA polymerase protein and 2b encoding viral silencing suppressor (112 aa), while RNA-3 encoding 3a movement protein (280 aa) and coat protein (219 aa) was 2223 nt in length. Phylogenetic analyses of nucleotide sequences of coleus CMV isolate is closely related to subgroup IB than to subgroup IA or II with other CMV isolates. In recombination analysis, the recombination event occurs between the subgroups of I, II as well as IA and IB in RNA 1, RNA2 and RNA3 of coleus isolate with other CMV isolates. To best of our knowledge, this is the first report of CMV infection in coleus.

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Section: Discussionmentioning

confidence: 99%

Characterization of cucumber mosaic virus infecting coleus (Plectranthus barbatus) in Karnataka

et al. 2019

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“…Similar results were obtained by Marini (1955) when he found CMV affecting basil naturally. Khan et al (2011) identified the natural occurrence of mosaic disease which was observed on basil (Ocimum sanctum L.) in Aligarh, India, during 2008; Cucumber mosaic virus (CMV) was detected by RT-PCR using coat protein gene specific primers of CMV.…”

Section: -Identification Of the Causal Pathogenmentioning

confidence: 99%

“…A survey of the literature revealed that the plant viruses known to infect basil worldwide are: Wintermantel and Natwick (2012) reported the natural occurrence of Alfalfa mosaic virus (AMV) on basil in California. Khan et al (2011) identified a new isolate of CMV subgroup II and designated as CMV-Basil as natural occurrence of mosaic disease in Aligarh, India. In India, the association of CMV with the mosaic disease of basil was investigated based on biological and serological characteristics (Raj et al, 1997).…”

Section: Introductionmentioning

confidence: 99%

Studies on Sweet Basil Mosaic Virus Disease and Its Control by Using Some Essential Oils

Saleh

Abdelkader

Eraky

2018

Scient. J. of Flowers and Ornament. Plants

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The current study, the mosaic virus disease of Ocimum basilicum L. (sweet basil) was identified and the different effects of three essential oils on growth, oil content and composition as well as reducing CMV disease symptoms of sweet basil plants in vivo. During 2012 season, natural occurrence of mosaic virus disease was observed on sweet basil in Assiut, Egypt. The causal agent of basil mosaic disease was detected as cucumber mosaic virus (CMV) by PCR product that revealed presence of approximately expected size (~ 781 bp) in naturally infected basil. Therefore, a field experiment was carried out during two successive seasons 2013 and 2014 on sweet basil plants at the Experimental Farm of Arab El Awamer Agricultural Research Station, Assiut Governorate, Egypt. Ten treatments of three essential oils extracted from lemongrass (Cymbopogon citratus), rosemary (Rosemarinus officinalis) and peppermint (Mentha piperita L.) plants were applied as foliar spray at 1% concentration before and after inoculation with isolated virus and two controls (healthy and infected plants) were arranged in a randomized complete block design with three replicates. Results revealed that application of essential oils under field condition improved growth and yield (branches, weight of fresh and dry plant (g/plant) as well as essential oil percentage), reduced the CMV disease symptoms compared to untreated infected control. As well as significant increase in total identified of essential oil composition of basil plant. Whereas the treatment with rosemary oil before and after inoculation respectively, was increased in myrcene, linalool when applied before inoculation and with a great increase in 1, 8-cineole when applied after inoculation. Also a great increase was related to peppermint oil treatment for eugenol, myrcene and 1, 8cineole % before inoculation comparable to untreated infected plants. While, using of peppermint oil significantly increased number of branches, fresh and dry weights, essential oil content and composition when applied before inoculation with virus compared to infected and healthy controls. Lemongrass essential oils exhibited a moderate protective effects. Generally, applying of rosemary essential oil as curative seemed to be superior for improving branches, weight of fresh and dry plant (g/plant), and essential oil percentage and composition comparing to other treatments.

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“…Since the CMV subgroup I was more virulent than subgroup it, is necessary to increase the awareness of the CMV occurrence in another plant. karantina yang diambil untuk meminimalkan kehilangan hasil akibat virus (Khan et al 2011a). Karakterisasi molekuler virus juga untuk mengetahui sejarah evolusinya (asal dan penyebarannya), sehingga dapat dilakukan pencegahan penyebarannya.…”

Section: Pendahuluanunclassified

“…Hasil penelitian Sulistyowati et al (2004) juga mengungkapkan jika pengelompokan 6 isolat CMV Australia pada pohon filogeni berkorelasi dengan identifikasi secara analisis homologi sekuen, serologi dan berdasarkan gejala. Identifikasi molekuler isolat baru CMV dari basil (Ocimum sanctum) di India menggunakan metode neighbour joining berdasarkan gen CP, berhasil mengidentifikasi isolat tersebut sebagai isolat baru CMV subgrup II (Khan et al 2011a).…”

Section: Perunutan Nukleotida Dan Analisis Sekuenunclassified

IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin)

Miftakhurohmah

Nyana

Damayanti

et al. 2017

j. penelitian tanam. industri

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Penyakit mosaik pada tanaman nilam di Indonesia berasosiasi dengan infeksi Telosma mosaic virus (TeMV), Broad bean wilt virus 2 (BBWV2), Cymbidium mosaic virus (CymMV) dan Cucumber mosaic virus (CMV). TeMV, BBWV2 dan CymMV sudah diidentifikasi secara molekuler, sedangkan CMV baru terdeteksi secara serologi. Karakte risasi molekuler setiap virus diperlukan sebagai salah satu dasar pengambilan tindakan pengendalian. Penelitian ini bertujuan untuk mengidentifikasi CMV asal tanaman nilam secara molekuler berdasarkan sekuen nukleotida gen CP. Tiga sampel daun nilam bergej ala terinfeksi virus diambil dari koleksi tanaman nilam di rumah kaca Balittro. Sampel daun diekstraksi asam nukleat totalnya (RNA+DNA). Asam nukleat total diamplifikasi dengan teknik one step reverse transcriptase polymerase chain reaction (RT-PCR) menggunakan primer spesifik gen coat protein (CP) CMV. Produk PCR berukuran 650 pb (pasang basa) dirunut sekuen nukleotidanya serta dianalisis homologi dan hubungan filogenetikanya dengan sekuen isolat-isolat CMV yang ada di GenBank. Sekuen nukleotida dan asam amino gen CP CMV asal nilam menunjukkan persentase kemiripan terbesar (97,1 dan 97,7%) dengan isolat um -Jepang. Analisis filogeni menunjukkan bahwa CMV asal nilam berkerabat sangat dekat dengan CMV isolat um-Jepang dengan nilai bootstrap 100%, dan berada dalam satu kelompok dengan isolat-isolat CMV subgrup IB. Keberadaan CMV subgrup IB pada tanaman nilam perlu diwaspadai karena subgrup I lebih virulen dibandingkan subgrup II. Penelitian ini merupakan laporan pertama karakterisasi molekuler CMV nilam, baik di Indonesia maupun di luar negeri, yang dapat digunakan sebagai salah satu dasar pengambilan tindakan pengendalian. Kata kunci: analisis homologi, pohon filogeni, nukleotida, asam amino ABSTRACT Mosaic disease on Indonesian patchouli associated with i nfection of TeMV, BBWV2, CymMV and CMV. TeMV, BBWV2 and CymMV has been identified molecularly, while CMV just was detected serologically. The objective of this study was to identify CMV from patchouli by molecular approach based on CP gene nucleotide sequence Leaf samples were collected from three mosaic symptomatic patchouli plants in greenhouse of Balittro. Leaf samples were extracted for the total nucleic acids (RNA + DNA). Nucleic acids were amplified using specific primer for CP gene of CMV by one step RT-PCR technique. The DNA of PCR product with the size of ~ 650 bp was directly sequenced and analyzed for its homology with sequences of CMV isolates extracted from Gene Bank. CMV CP gene from patchouli showed the highest of

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Molecular identification of a new isolate of Cucumber mosaic virus subgroup II from basil (Ocimum sanctum) in India

Cited by 9 publications

References 8 publications

Characterization of cucumber mosaic virus infecting coleus (Plectranthus barbatus) in Karnataka

Characterization of cucumber mosaic virus infecting coleus (Plectranthus barbatus) in Karnataka

Studies on Sweet Basil Mosaic Virus Disease and Its Control by Using Some Essential Oils

IDENTIFIKASI MOLEKULER CUCUMBER MOSAIC VIRUS (CMV) ASAL TANAMAN NILAM (Pogostemon cablin) / Molecular Identification of Cucumber Mosaic Virus Infecting Patchouli (Pogostemon cablin)

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