Molecular identification of adenoviruses associated with respiratory infection in Egypt from 2003 to 2010

Demian, Pola Nashdy; Horton, Katherine C.; Kajon, Adriana E.; Siam, Rania; Hasanin, Amel Mohamed Nageib; Sheta, Amany Elgohary; Cornelius, Claire; Gaynor, Anne

doi:10.1186/1471-2334-14-50

Cited by 28 publications

(34 citation statements)

References 44 publications

(52 reference statements)

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“…In addition to Beijing, these HAdVs were also most frequently detected from acute respiratory tract disease cases in other cities of China such as Guangzhou in Central South China [18] and Lanzhou City in Northeast China [19], which indicated that these HAdV types are widely distributed in China. This study is also consistent with reports from Argentina [20], USA [21], Egypt [22], and Korea [23].…”

Section: Discussionsupporting

confidence: 92%

Adenoviruses Associated with Acute Respiratory Diseases Reported in Beijing from 2011 to 2013

et al. 2015

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BackgroundAdenovirus is one of the most common causes of viral acute respiratory infections. To identify the types of human adenoviruses (HAdVs) causing respiratory illness in Beijing, a sentinel surveillance project on the viral aetiology of acute respiratory infection was initiated in 2011.Principal findingsThrough the surveillance project, 4617 cases of respiratory infections were identified during 2011-2013. Throat swabs (pharynx and tonsil secretions) were collected from all the patients, and 15 different respiratory viruses were screened by multiplex one-step PCR method. 45 were identified as adenovirus-positive from sporadic and outbreak cases of respiratory infection by a multiplex one-step RT-PCR method, and a total of 21 adenovirus isolates were obtained. Five HAdV types among three species, including HAdV-3 (species HAdV-B), HAdV-4 (species HAdV-E), HAdV-7 (species HAdV-B), HAdV-55 (species HAdV-B), and an undefined HAdV type (species HAdV-C) were identified. The comparison results of the penton base, hexon, and fiber gene sequences of the Beijing HAdV-3, HAdV-4, HAdV-7, and HAdV-55 strains in this study and those from the GenBank database indicated significant spatial and temporal conservation and stability of sequences within the genome; however, the phylogenetic relationship indicated that both strain BJ04 and strain BJ09 isolated in 2012 and 2013, respectively, may have recombined between HAdV-1 genome and HAdV-2 genome within species HAdV-C, indicating intraspecies recombination.ConclusionsThis study confirmed that at least 5 HAdV types including HAdV-3, HAdV-4, HAdV-7, HAdV-55 and an undefined HAdV type were co-circulating and were the causative agents of respiratory tract infections in recent years in Beijing. HAdV-3, HAdV-4, HAdV-7, and HAdV-55 showed the apparent stability of the genomes, while intraspecies recombination was identified in strain BJ04 and BJ09. The recombinants carrying penton base gene of HAdV-1 as well as hexon and fiber genes of HAdV-2 might be a novel type of HAdV worthy of further study.

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Section: Discussionsupporting

confidence: 92%

Adenoviruses Associated with Acute Respiratory Diseases Reported in Beijing from 2011 to 2013

et al. 2015

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“…Regarding the group age, as expected, results showed that most patients with HAdV infection were younger than 5 years (62.2%), a statistically significant finding. These results are in concordance with those of other studies which findings concluded that most children are infected by adenovirus at an early age [25,37,[38][39][40][41]. Indeed, it is well established that by 5 years of age, 70% to 80% of children demonstrate antibodies to at least one serotype [42].…”

Section: Discussionsupporting

confidence: 92%

Respiratory viruses in patients with influenza-like illness in Senegal: Focus on human respiratory adenoviruses

et al. 2017

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BackgroundHuman adenoviruses (HAdVs) are highly contagious pathogens that are associated with a wide spectrum of human illnesses involving the respiratory tract. In the present study, we investigate the epidemiologic and viral molecular features of HAdVs circulating in Senegal after 4 consecutive years of sentinel surveillance of influenza-like Illness cases.Methodology and resultsFrom January 2012 to December 2015 swabs were collected from consenting ILI outpatients. Adenoviral detection is performed by rRT-PCR with the Anyplex™ II RV16 Detection kit (Seegene) and molecular characterization was performed using a partial hexon gene sequence. 6381 samples were collected. More than half of patients (51.7%; 3297/6381) were children of ≤ 5 years. 1967 (30.8%) were positive for HAdV with 1561 (79.4%) found in co-infection with at least one another respiratory virus. The most common co-detections were with influenza viruses (53.1%; 1045/1967), rhinoviruses (30%; 591/1967), enteroviruses (18.5%; 364/1967) and RSV (13.5%; 266/1967). Children under 5 were the most infected group (62.2%; 1224/1967; p <0.05). We noted that HAdV was detected throughout the year at a high level with detection peaks of different amplitudes without any clear seasonality. Phylogenetic analysis revealed species HAdV-C in majority, species HAdV-B and one HAdV- 4 genome type. The 9 HAdV-B species like strains from Senegal grouped with genome types HAdV-7, HAdV-55 and HAdV-11 as shown by a phylogenetic branch with a high bootstrap value of (88%).ConclusionIn conclusion, the results of the present study suggest strong year-round HAdV activity in Senegal, especially in children up to 5 years of age. Molecular studies revealed that the dominant species in circulation in patients with ILI appears to be HAdV-C and HAdV-B species. The circulation of though HAdV-7 and HAdV-55 genome types is of note as these serotypes are recognized causes of more severe and even fatal acute respiratory infections.

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“…Taken together, this is evidence that these two definitions produce physically different outbreaks and emphasizes the importance of taking definitions into account when comparing outbreaks. Given that it is known that influenza-like-illness outbreaks commonly include a number of viruses other than the flu, including adenoviruses and rhinovirus [ 32 – 34 ], and that Eick-Cost et al demonstrates low specificity within the DoD definition [ 6 ], it makes sense that the two definitions produce dissimilar outbreaks.…”

Section: Resultsmentioning

confidence: 99%

Novel Use of Flu Surveillance Data: Evaluating Potential of Sentinel Populations for Early Detection of Influenza Outbreaks

et al. 2016

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Influenza causes significant morbidity and mortality each year, with 2–8% of weekly outpatient visits around the United States for influenza-like-illness (ILI) during the peak of the season. Effective use of existing flu surveillance data allows officials to understand and predict current flu outbreaks and can contribute to reductions in influenza morbidity and mortality. Previous work used the 2009–2010 influenza season to investigate the possibility of using existing military and civilian surveillance systems to improve early detection of flu outbreaks. Results suggested that civilian surveillance could help predict outbreak trajectory in local military installations. To further test that hypothesis, we compare pairs of civilian and military outbreaks in seven locations between 2000 and 2013. We find no predictive relationship between outbreak peaks or time series of paired outbreaks. This larger study does not find evidence to support the hypothesis that civilian data can be used as sentinel surveillance for military installations. We additionally investigate the effect of modifying the ILI case definition between the standard Department of Defense definition, a more specific definition proposed in literature, and confirmed Influenza A. We find that case definition heavily impacts results. This study thus highlights the importance of careful selection of case definition, and appropriate consideration of case definition in the interpretation of results.

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Molecular identification of adenoviruses associated with respiratory infection in Egypt from 2003 to 2010

Cited by 28 publications

References 44 publications

Adenoviruses Associated with Acute Respiratory Diseases Reported in Beijing from 2011 to 2013

Adenoviruses Associated with Acute Respiratory Diseases Reported in Beijing from 2011 to 2013

Respiratory viruses in patients with influenza-like illness in Senegal: Focus on human respiratory adenoviruses

Novel Use of Flu Surveillance Data: Evaluating Potential of Sentinel Populations for Early Detection of Influenza Outbreaks

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