Molecular identification of Aphidius transcaspicus (Hymenoptera: Braconidae: Aphidiinae), a closely associated species of Aphidius colemani, in Korea

Hwang, Hwal‐Su; Jung, Duck-Oung; Kim, Jin-Won; Lee, Kyeong-Yeoll

doi:10.1016/j.aspen.2018.09.009

Cited by 9 publications

(6 citation statements)

References 15 publications

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“…Identification based on DNA barcoding corresponds to that based on traditional morphological taxonomy 18,19,31,32 . However, the DNA barcoding sequences of certain Aphidiinae species are difficult to differentiate because of their relatively low intraspecific and interspecific diversity 33–35 . We found the sequence similarity was >98% between the COI barcode sequences for A. gifuensis in present study and those of A. rhopalosiphi , A. ervi , A. microlophii , and A. urticae in the BOLD and NCBI databases.…”

Section: Discussionsupporting

confidence: 66%

Early monitoring of parasitism by Aphidiinae parasitoids on the grain aphidSitobion miscanthiin wheat fields usingDNAbarcoding

Peng

Wang

et al. 2022

Pest Management Science

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BACKGROUND: Sitobion miscanthi is a major wheat pest at the grain-filling stage found in China. Identifying parasitoid species and understanding parasitism rates are keys to controlling the aphids via natural enemies in the wheat field. RESULTS:In the present study, a method based on DNA barcoding for early determination of the community composition of Aphidiinae parasitoids and parasitism on the aphid was developed. The proposed method detected Aphidius gifuensis as the predominant parasite, with parasitism rates of 40.1 ± 2.8% in 2019 and 65.7 ± 3.7% in 2022, and found that the rate varied significantly among different wheat varieties. COI primers efficiently amplified the Aphidiinae parasitoids COI fragments and amplified the aphid COI fragments derived from parasitized (mummified) S. miscanthi. Thus, the COI barcode is not sufficiently specific to unambiguously detect immature parasitoids inside their S. miscanthi hosts. However, it can be used to detect the DNA extracted from mummified aphids. In contrast, the 16S and LWRh primers effectively amplified and identified the parasitoids in parasitized aphids. The 16S primer was reliable even in the early stages of parasitism (24 h) and for DNA samples stored at −20 °C for 5 days. The three barcodes from COI, 16S, and LWRh genes could not clearly distinguish a few certain Aphidiinae species owing to relatively low intraspecific and interspecific diversity. CONCLUSION: The morphological features remain indispensable when identifying Aphidiinae species. Nonetheless, the COI and 16S primers could be used in combination for monitoring the parasitism rates on S. miscanthi in wheat fields.

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Section: Discussionsupporting

confidence: 66%

Early monitoring of parasitism by Aphidiinae parasitoids on the grain aphidSitobion miscanthiin wheat fields usingDNAbarcoding

Peng

Wang

et al. 2022

Pest Management Science

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“…Here, we use DNA barcoding to describe three new Aphidius species from the Korean Peninsula, an area with a poorly known aphidiine fauna that includes only 58 known species (Starý and Choi 2000;Starý et al 2001;Yu et al 2016;Choi et al 2017;Choi and Kim 2018;Hwang et al 2018;Kim et al 2020), which is not consistent with their economic and ecological importance. Aphidius longicarpus sp.…”

Section: Discussionmentioning

confidence: 99%

“…Members of the genus Aphidius are medium-sized wasps, only 15 species of which have been recorded in South Korea (Starý and Choi 2000;Yu et al 2016;Hwang et al 2018;NIBR 2019;Kim et al 2020). Since Aphidius species such as A. colemani and A. ervi are used as imported biocontrol agents against pest aphids worldwide, especially in agriculture and horticulture (Sequeira and Mackauer 1992;Henter and Via 1995;Fernandez and Nentwig 1997;Takada 1998), it is important to explore indigenous Aphidius species and to confirm their diversity in South Korea and surrounding regions.…”

Section: Introductionmentioning

confidence: 99%

Three new species of the genus Aphidius (Hymenoptera, Braconidae, Aphidiinae) from South Korea

Kim¹,

Tomanović²,

Yu³

et al. 2021

JHR

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Several species of the genus Aphidius are well known as commercial biocontrol agents of pest aphids, and more than 130 species of the genus have been recorded worldwide. To date, only 15 Aphidius species have been recorded in South Korea. Using the DNA barcode region (ca. 658 bp) of mitochondrial cytochrome c oxidase subunit I (COI), we amplified sequences of 15 Aphidius Korean species, aligned them in combination with 23 reference species retrieved from GenBank for comparison and identification, and then reconstructed a barcode phylogeny by the neighbour-joining method. As a result, three Aphidius species were found to be new to Science. Descriptions and illustrations of the three species new to Science – Aphidius longicarpussp. nov., A. longistigmussp. nov., and A. asiaticussp. nov. – are provided, together with their phylogenetic position within the genus Aphidius. In addition, a redescription of A. areolatus, a parasitoid of maple aphids (Peryphillus spp.), is also given.

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“…Though it is currently unclear whether they studied A. colemani per se, as the identity of this species is not clear yet, it is possible that they have also studied A. platensis, as A. colemani is now a complex of cryptic, sibling species [ 49 , 75 , 76 ]. It was considered as a group, together with two other close species: A. transcaspicus and A. platensis , probably used as synonyms in several taxonomic descriptions [ 52 , 55 , 77 ]. Host fidelity is maximized in constant environments, where a continuous supply of aphid hosts is found [ 37 ]; e.g., parasitoids from the aphid hosts S. avenae, Acyrthosiphon pisum Harris (Hemiptera: Aphididae) alfalfa race and A. pisum pea race showed a preference for their natal host [ 51 ].…”

Section: Discussionmentioning

confidence: 99%

“…The other population came from M. persicae mummies, collected at the beginning of the spring season (September–October) from peach orchards (Duao 35°34′23.23″ S and 71°33′09.53″ W 183 m). Parasitoids were determined using taxonomic keys [ 49 , 52 ] and once the population was established, a molecular approach with partial sequences of mtCOI [ 52 , 55 ] was carried out on the progeny to confirm the determination of the species. Parasitoids were fed with 30% honey solution through soaked cotton wicks and water.…”

Section: Methodsmentioning

confidence: 99%

Suitability and Profitability of a Cereal Aphid for the Parasitoid Aphidius platensis in the Context of Conservation Biological Control of Myzus persicae in Orchards

Alvarez-Baca

Alfaro-Tapia

Lavandero

et al. 2020

Insects

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The use of cover crops can promote the abundance and early arrival of populations of natural enemies. Cereal cover crops between orchards rows could encourage the early arrival of the parasitoid Aphidius platensis, as they offer alternative winter hosts (e.g., Rhopalosiphum padi), enhancing the control of Myzus persicae in spring. However, the preference for and suitability of the alternative host must be addressed beforehand. To evaluate the potential of this strategy, we assessed host preference using behavioural choice tests, as well as no-choice tests measuring fitness traits, when developing on both host species. One source field for each aphid population from the above hosts was chosen. There was a clear choice for R. padi compared to M persicae, independently of the source, probably due to more defensive behaviours of M. persicae (i.e., kicks and escapes). Nevertheless, both aphid species were suitable for parasitoids’ development. The female progeny developed on R. padi were larger in size, irrespective of their origin. According to our results, in peach orchards with cereals sown between peach trees during the autumn, where we expect when R. padi populations will no longer be available during spring, A. platensis should be able to switch to M. persicae.

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Molecular identification of Aphidius transcaspicus (Hymenoptera: Braconidae: Aphidiinae), a closely associated species of Aphidius colemani, in Korea

Cited by 9 publications

References 15 publications

Early monitoring of parasitism by Aphidiinae parasitoids on the grain aphidSitobion miscanthiin wheat fields usingDNAbarcoding

Early monitoring of parasitism by Aphidiinae parasitoids on the grain aphidSitobion miscanthiin wheat fields usingDNAbarcoding

Three new species of the genus Aphidius (Hymenoptera, Braconidae, Aphidiinae) from South Korea

Suitability and Profitability of a Cereal Aphid for the Parasitoid Aphidius platensis in the Context of Conservation Biological Control of Myzus persicae in Orchards

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