Molecular identification of bamboo genera and species based on RAPD-RFLP markers

Konzen, Enéas Ricardo; Peron, Raquel; Ito, M. A.; Brondani, Gilvano Ebling; Tsai, Siu Mui

doi:10.14214/sf.1691

Cited by 22 publications

(17 citation statements)

References 27 publications

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“…The value of PIC produced in this study ranged from 0.90 to 0.98. Our result was higher than Konzen et al's (2017) RAPD, where PIC ranged from 0.00 to 0.94. However, our observations were in coherence with the PIC results from Desai et al (2015).…”

Section: Rapd Primer Screening and Amplification Profile Analysis Rapcontrasting

confidence: 76%

“…Total 16 unique bands (1.8% from the total) were characterised. This percentage number is lower compared with the result of Konzen et al (2017).…”

Section: Unique Bandscontrasting

confidence: 68%

“…The DNA bands were made into matrices 1 and 0, 1 if there was a band and 0 otherwise. The principal coordinate analysis (PCoA) was done by using Software PAST (Hammer et al 2001;Konzen et al 2017).…”

Section: Discussionmentioning

confidence: 99%

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Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Annisa

Hafzari

Setiawati

et al. 2019

Folia Forestalia Polonica

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Conservation of bamboos for future exploitation as fuel, fibre and as an ingredient for cosmetics depends on knowledge of its natural genetic variation. The study of molecular genetic diversity in bamboos will provide important information for its conservation. This article reports on the genetic diversity in 25 species representing five genera of bamboos found in Indonesia using Random Amplified Polymorphic DNA (RAPD) molecular markers. Out of 40 primers, 24 primers produced 1107 total bands and 86.21% of polymorphic bands across the 25 species. Sixteen bands were uniquely found in one species only and their presence or absence helped to define nine bamboo species. RAPD band sizes ranged from 162 to 2247 base pairs. A dendrogram based on the similarity coefficient of Dice divided the bamboo species into three big clusters. In conclusion, RAPD can capture the diversity among five different bamboo genera and has a great potential to be used in the study of genetic diversity in Indonesian bamboos.

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Section: Rapd Primer Screening and Amplification Profile Analysis Rapcontrasting

confidence: 76%

“…Total 16 unique bands (1.8% from the total) were characterised. This percentage number is lower compared with the result of Konzen et al (2017).…”

Section: Unique Bandscontrasting

confidence: 68%

See 1 more Smart Citation

Evaluation of RAPD markers for molecular identification of five bamboo genera from Indonesia

Annisa

Hafzari

Setiawati

et al. 2019

Folia Forestalia Polonica

View full text Add to dashboard Cite

show abstract

Section: Molecular Markersmentioning

confidence: 99%

“…The markers clearly clustered ten cultivars into 3 groups, highlighting the genetic resemblance between cultivars in each group. Afterward, combined use of marker systems has been used in several studies such as RAPD‐AFLP (Nag et al., 2013), RAPD‐ISSR (Desai et al., 2015), RAPD‐RFLP (Konzen, Perón, Ito, Brondani, & Tsai, 2017), RAPD with morphological traits (Shalini, Meena, Tarafdar, & Thakur, 2013), and morphological traits with sequence‐related amplified polymorphism (SRAP) markers (Zhu, Liu, Tang, Fu, & Tang, 2014). Genetic diversity between cultivars of Phyllostachys violascens was reported using combinations of ISSR, AFLP, and SRAP markers (Lin et al., 2011).…”

Section: Prospects For Genomics‐assisted Breedingmentioning

confidence: 99%