Molecular identification of Candida auris by PCR amplification of species-specific GPI protein-encoding genes

Ruiz-Gaitán, Alba; Fernández-Pereira, Jordan; Valentı́n, Eulogio; Tormo-Más, María Ángeles; Eraso, Elena; Pemán, Javier; Groot, Piet W. J. de

doi:10.1016/j.ijmm.2018.06.014

Cited by 40 publications

(32 citation statements)

References 31 publications

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“…(Figure 3b). The second method used to identify C. auris was an specific amplification of the gpi gene [36]. This method did not revealed the presence of C. auris among the 3 isolates (Figure 3c).…”

Section: Identification Of Cryptic Species Within the Candida Albicanmentioning

confidence: 99%

“…PCR of the gpi gene for identification of C. auris A PCR assay described for the rapid identification of C. auris was also performed on the isolates of the C. haemulonii complex. This technique amplified unique glycosylphosphatidylinositol (GPI) protein-encoding genes [36]. A standard PCR mix was prepared with 12.5 µL of 2X PCR Master Mix, 1 µL of each primer: 03410_F: 5´ -GCC GCT AGA TTG ATC ACC GT-3, and 03410_R: 5-TAG GTG TGG GTA CCC TTG GT -3, 9 µL of water, and 1.5 µL of DNA template.…”

Section: Pcr-rflp Of the Its Region Using Alui For Identification Of mentioning

confidence: 99%

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Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha¹,

Montes²,

Ortiz³

et al. 2019

Preprint

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Candida spp. are the most common cause of fungal infections worldwide. The taxonomy of Candida is controversial and has undergone recent changes due to novel genetically related species. Therefore, some complexes of cryptic species have been proposed. In clinical settings, the correct identification of Candida species is relevant since some species are associated with high resistance to antifungal drugs and increased virulence. This study aimed to identify the species of four Candida complexes (C. albicans, C. glabrata, C. parapsilosis, and C. haemulonii) by molecular methods. This is the first report of six cryptic Candida species in Honduras: C. dubliniensis, C. africana, C. duobushaemulonii, C. orthopsilosis, and C. metapsilosis, and it is also the first report of the allele hwp1-2 of C. albicans sensu stricto. It was not possible to demonstrate the existence of C. auris among the isolates of the C. haemulonii complex. We also propose a simple method based on PCR-RFLP for the discrimination of the multi-resistant pathogen C. auris within the C. haemulonii complex.

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Section: Identification Of Cryptic Species Within the Candida Albicanmentioning

confidence: 99%

Section: Pcr-rflp Of the Its Region Using Alui For Identification Of mentioning

confidence: 99%

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha¹,

Montes²,

Ortiz³

et al. 2019

Preprint

View full text Add to dashboard Cite

show abstract

“…A reference strain was used as a positive control in all assays (Figure 3b). The second method used to identify C. auris was a specific amplification of the gpi gene [38]. This method did not reveal the presence of C. auris among the three isolates (Figure 3c).…”

Section: Identification Of Cryptic Species Of the C Haemulonii Complexmentioning

confidence: 99%

“…A PCR assay described for the rapid identification of C. auris was also performed on the isolates of the C. haemulonii complex. This technique amplified a unique glycosylphosphatidylinositol (GPI) protein-encoding gene originally described as QG37_03410 [38]. A standard PCR mix was prepared with 12.5 µL of 2× PCR Master Mix, 1 µL of each primer: 03410_F: 5′-GCCGCTAGATTGATCACCGT-3′ and 03410_R: 5′-TAGGTGTGGGTACCCTTGGT-3′, 9 µL of water, and 1.5 µL of DNA template.…”

Section: Pcr Of the Gpi Gene For Identification Of C Aurismentioning

confidence: 99%

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Fontecha

Montes

Ortiz

et al. 2019

JoF

View full text Add to dashboard Cite

Candida spp. are one of the most common causes of fungal infections worldwide. The taxonomy of Candida is controversial and has undergone recent changes due to novel genetically related species. Therefore, some complexes of cryptic species have been proposed. In clinical settings, the correct identification of Candida species is relevant since some species are associated with high resistance to antifungal drugs and increased virulence. This study aimed to identify the species of four Candida complexes (C. albicans, C. glabrata, C. parapsilosis, and C. haemulonii) by molecular methods. This is the first report of six cryptic Candida species in Honduras: C. dubliniensis, C. africana, C. duobushaemulonii, C. orthopsilosis, and C. metapsilosis, and it is also the first report of the allele hwp1-2 of C. albicans sensu stricto. It was not possible to demonstrate the existence of C. auris among the isolates of the C. haemulonii complex. We also propose a simple method based on PCR-RFLP for the discrimination of the multi-resistant pathogen C. auris within the C. haemulonii complex.

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“…Molecular methods, such as sequencing of the ITS and D1/D2 regions of the 28S ribosomal DNA, PCR, real‐time PCR and AFLP, can also reliably identify and confirm C. auris . PCR assays based on the unique GPI protein encoding and ITS2 genes have claimed accurate identification of C. auris . Recently, GPS MONODOSE dtec‐qPCR kit (Alicante, Spain) for the detection of C. auris have been introduced with many advantages compared to other PCR methods.…”

Section: Identificationmentioning

confidence: 99%

Candida auris—the growing menace to global health

Lone

Ahmad

2019

Mycoses

174

119

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summary A paradigm shift of candidiasis from Candida albicans to non‐albicans Candida species has fundamentally increased with the advent of C. auris. C. auris, despite being a newly emerged multidrug‐resistant fungal pathogen, is associated with severe invasive infections and outbreaks with high mortality rates. Initially reported from Japan in 2009, C. auris have now been found in different countries on all the continents except Antarctica. Due to its capability of nosocomial transmission and forming adherent biofilms on clinically important substrates, a high number of related hospital outbreaks have been reported worldwide. As C. auris is a multidrug‐resistant pathogen and is prone to misidentification by available conventional methods, it becomes difficult to detect and manage C. auris infection and also limits the therapeutic options against this deadly pathogen. The emergence of multidrug‐resistant C. auris advocates and amplifies the vigilance of early diagnosis and appropriate treatment of fungal infections. In this review, we discussed the nine‐year‐old history of C. auris—its trends in global emergence, epidemiological relatedness, isolation, mortality, associated risk factors, virulence factors, drug resistance and susceptibility testing, diagnostic challenges, microbiological characteristics, therapeutic options and infection prevention and control associated with this pathogen.

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Molecular identification of Candida auris by PCR amplification of species-specific GPI protein-encoding genes

Cited by 40 publications

References 31 publications

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Identification of Cryptic Species of Four Candida Complexes in a Culture Collection

Candida auris—the growing menace to global health

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