Molecular identification of Cryptosporidium spp. from animal sources in China

Yin, Jun; Yuan, Zhongying; Shen, Yujuan; Zhang, Jing; Jiang, Yanyan; Cao, Jianping

doi:10.3855/jidc.3540

Cited by 9 publications

(11 citation statements)

References 17 publications

(18 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Among them, C. parvum is the most common zoonotic Cryptosporidium species and has been found in humans worldwide [10, 29]. It is a zoonotic pathogen and has been reported in humans as well as in many animals in China, such as cattle, dogs, horses, birds, giant pandas, red pandas, deer, snakes, and rodents [15, 27, 30]. Cryptosporidium rat genotype II has been found in brown rats in the Philippines [20], Nigeria [1], Australia [21], and China.…”

Section: Discussionmentioning

confidence: 99%

First detection of Cryptosporidium spp. in red-bellied tree squirrels (Callosciurus erythraeus) in China

et al. 2019

View full text Add to dashboard Cite

Cryptosporidium spp. are opportunistic pathogens that cause diarrhea in a variety of animal hosts. Although they have been reported in many animals, no information has been published on the occurrence of Cryptosporidium spp. in red-bellied tree squirrels (Callosciurus erythraeus). A total of 287 fecal specimens were collected from Sichuan province in China; the prevalence of Cryptosporidium spp., measured by nested-PCR amplification of the partial small-subunit (SSU) rRNA gene, was 1.4% (4/287). Three different Cryptosporidium species or genotypes were identified: Cryptosporidium parvum (n = 1), Cryptosporidium wrairi (n = 1), and Cryptosporidium rat genotype II (n = 2). The present study is the first report of Cryptosporidium infection in red-bellied tree squirrels in China. Although there is a relatively low occurrence of Cryptosporidium, the presence of C. parvum and C. wrairi, which were previously reported in humans, indicates that red-bellied tree squirrels may be a source of zoonotic cryptosporidiosis in China.

show abstract

Section: Discussionmentioning

confidence: 99%

First detection of Cryptosporidium spp. in red-bellied tree squirrels (Callosciurus erythraeus) in China

et al. 2019

View full text Add to dashboard Cite

show abstract

“…Cryptosporidiosis is suspected to be endemic in dogs across China even though only few extensive studies had been conducted. From 84 fecal samples taken from cats, dogs and from waste water of zoo drainage channels, in a study in Shanghai, China, an infection rate of 7% was reported [112]. From 545 fecal samples taken from yaks in nine different counties of central western region of China, the prevalence of Cryptosporidium spp.…”

Section: Chinamentioning

confidence: 99%

Prevalence and risk factors for cryptosporidiosis: a global, emerging, neglected zoonosis

Bamaiyi

Redhuan

2016

Asian Biomedicine

View full text Add to dashboard Cite

Background: Cryptosporidiosis is a zoonotic disease caused by the important parasitic diarrheal agent Cryptosporidium spp. Cryptosporidiosis occurs in all classes of animals and man with a rapidly expanding host range and increased importance since the occurrence of human immunodeficiency virus/acquired immunodeficiency syndrome in man. Objectives: To review the global picture of cryptosporidiosis in man and animals with emphasis on prevalence and risk factors. Methods: Current relevant literature on cryptosporidiosis was reviewed. Results: Cryptosporidiosis is widely distributed and the risk factors vary from one region to another with hygiene and immune status as important risk factors. Conclusions: Cryptosporidium spp. associated mortality has not only been reported in immune-compromised patients, but also in immune-competent patients. Yet in many countries not much attention is paid to the control and prevention of this infection in animals and man. The neglect of this disease despite the serious threat it poses to animals, their husbandry, and humans, has led the World Health Organization to list it among globally neglected diseases. To control and prevent this infection more effort needs to be directed at controlling the risk factors of the infection in man and animals.

show abstract

“…The remaining four nucleotide sequences (KF993721, KF993722, KF993725, and KF993727) were novel. Although two (KF993721 and KF993722) differed at two base positions, both were identical (at the amino acid level) with previous human isolates in China [24].…”

Section: Genetic Characterization Of Assemblages C D E and Fmentioning

confidence: 56%

“…Meanwhile, more attention should be paid to the health of protected animal species and endangered/threatened animal species in China. We have previously molecularly identified Cryptosporidium in pets and zoo animals [24]; it is also necessary to detect other intestinal pathogens in these animals. The present study mainly focused on the molecular epidemiological investigation of G. duodenalis in pet and zoo animals from Shanghai, China.…”

Section: Introductionmentioning

confidence: 99%

Occurrence and multilocus genotyping of Giardia duodenalis in pets and zoo animals in Shanghai, China

Liu

Shen

Liu

et al. 2017

J Infect Dev Ctries

Self Cite

View full text Add to dashboard Cite

Introduction: High prevalence of Giardia infections occurs in humans and animals, partly because of the increasing numbers of pets. We determined the presence and genotypes of G. duodenalis in pets and zoo animals. Methodology: A total of 84 specimens were collected from dogs and cats from a pet hospital, and 54 specimens from a zoo, which included deer, tigers, yaks, and others. All the specimens were examined by microscopy and by polymerase chain reaction (PCR) amplification and subsequent sequencing of glutamate dehydrogenase (gdh), beta-giardin (bg), and triose phosphate isomerase (tpi) genes. Results: Giardia infection was confirmed in 5.95% and 15.48% of animals by microscopy and by PCR, respectively; the detection levels were 13.33% and 26.67% for pets, and 1.85% and 9.26% for zoo animals. Four assemblages were identified: assemblage C in dogs, cats, and a sheep; D in dogs, a wolf, a yak, and a leopard; E in a sheep; and F in a cat and a leopard. PCR gave the highest amplification rate at the gdh locus. Eight, five, and four sequences were novel at the gdh, bg, and tpi loci, respectively. Two tpi sequences of dog-derived assemblage C had 100% homology with amino acid sequences from human-derived isolates. Conclusions: The molecular characterization of G. duodenalis in pets and zoo animals in China is described. Assemblage D was identified in a yak and a leopard for the first time. Multilocus genotyping analysis identified the same tpi gene sequences of assemblage C in dogs and humans, indicating potential zoonotic transmission.

show abstract

Molecular identification of Cryptosporidium spp. from animal sources in China

Abstract: This item has no abstract. Follow the links below to access the full text.

Cited by 9 publications

References 17 publications

First detection of Cryptosporidium spp. in red-bellied tree squirrels (Callosciurus erythraeus) in China

First detection of Cryptosporidium spp. in red-bellied tree squirrels (Callosciurus erythraeus) in China

Prevalence and risk factors for cryptosporidiosis: a global, emerging, neglected zoonosis

Occurrence and multilocus genotyping of Giardia duodenalis in pets and zoo animals in Shanghai, China

Contact Info

Product

Resources

About