Molecular identification of four <scp>P</scp>anonychus species (<scp>A</scp>cari: <scp>T</scp>etranychidae) in <scp>K</scp>orea, including new records of <scp>P</scp>. caglei and <scp>P</scp>. mori

Khaing, Tin Moe; Shim, Jooyong; Lee, Kyeong-Yeoll

doi:10.1111/1748-5967.12135

Cited by 4 publications

(1 citation statement)

References 34 publications

(50 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Osakabe, 1987 Gotoh, 1992Gotoh et al, 2007Arabuli et al, 2019Kajiwara et al, 2016Ben-David et al, 2007Hinomoto et al, 2007Matsuda et al, 2012Matsuda et al, 2013Arabuli et al, 2019Osakabe et al, 2008Arimoto et al, 2013Hurtado et al, 2008Ovalle et al, 2020Khaing et al, 2015Shim et al, 2016Zélé et al, 2018Sinaie et al, 2018Li et al, 2015 as Oligonychus and Amphitetranychus). However, some closely related species (e. g., from genus Tetranychus) may be indistinguishable from each other by their barcode se quences, as it has been shown for T. kanzawai, T. parakanzawai, and T. ezoensis having similar ITS sequences (Matsuda et al, 2013); and T. urticae and T. turkestani having similar COI gene sequences (Hinomoto et al, 2007;Konoplev et al, 2017).…”

Section: Lamp Genus Speciesmentioning

confidence: 99%

Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods

et al. 2023

View full text Add to dashboard Cite

Spider mites (Acari: Tetranychidae) are dangerous pests of agricultural and ornamental crops, the most economically significant of them belonging to the genera Tetranychus, Eutetranychus, Oligonychus and Panonychus. The expansion of the distribution areas, the increased harmfulness and dangerous status of certain species in the family Tetranychidae and their invasion of new regions pose a serious threat to the phytosanitary status of agroand biocenoses. Various approaches to acarofauna species diagnosis determine a rather diverse range of currently existing methods generally described in this review. Identification of spider mites by morphological traits, which is currently considered the main method, is complicated due to the complexity of preparing biomaterials for diagnosis and a limited number of diagnostic signs. In this regard, biochemical and molecular genetic methods such as allozyme analysis, DNA barcoding, restriction fragment length polymorphism (PCR-RFLP), selection of species-specific primers and real-time PCR are becoming important. In the review, close attention is paid to the successful use of these methods for species discrimination in the mites of the subfamily Tetranychinae. For some species, e. g., the two-spotted spider mite (Tetranychus urticae), a range of identification methods has been developed – from allozyme analysis to loop isothermal amplification (LAMP), while for many other species a much smaller variety of approaches is available. The greatest accuracy in the identification of spider mites can be achieved using a combination of several methods, e. g., examination of morphological features and one of the molecular approaches (DNA barcoding, PCR-RFLP, etc.). This review may be useful to specialists who are in search of an effective system for spider mite species identification as well as when developing new test systems relevant to specific plant crops or a specific region.

show abstract

Section: Lamp Genus Speciesmentioning

confidence: 99%

Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods

et al. 2023

View full text Add to dashboard Cite

show abstract

Investigating species boundaries using DNA and morphology in the mite Tyrophagus curvipenis (Acari: Acaridae), an emerging invasive pest, with a molecular phylogeny of the genus Tyrophagus

et al. 2018

View full text Add to dashboard Cite

Mites of the genus Tyrophagus (Acari: Acaridae) are among the most widespread and common mites, inhabiting diverse natural and anthropogenic habitats. Some species are pests of agricultural products and stored food and/or live in house dust, causing allergies to humans. We sequenced 1.2 kb of the mitochondrial COI gene for 38 individuals belonging to seven species of Tyrophagus, including T. curvipenis, T. putrescentiae, T. fanetzhangorum, T. longior, T. perniciosus, and T. cf. similis. Molecular phylogenetic analyses (1) recovered two major clades corresponding to the presence or absence of eyespots, and (2) separated all included morphological species. Tyrophagus curvipenis and T. putrescentiae had the lowest between-species genetic distances (range, mean ± SD): 14.20-16.30, 15.17 ± 0.40 (K2P). The highest within-species variation was found in T. putrescentiae 0.00-4.33, 1.78 ± 1.44 (K2P). In this species, we recovered two distinct groups; however, no geographical or ecological dissimilarities were observed between them. Based on our analyses, we document important morphological differences between T. curvipenis and T. putrescentiae. For the first time, we record the occurrence of T. curvipenis in the New World and suggest that it may be an emerging pest as it is currently spreading in agricultural produce.

show abstract

DNA Barcoding and Phylogeny of Acari Species Based on ITS and COI Markers

Pérez-Sayas

Piña

Sabater-Muñoz

et al. 2022

Journal of Zoological Systematics and Evolutionary Research

View full text Add to dashboard Cite

Acari harbor numerous minute species of agricultural economic importance, mainly Tetranychidae and Phytoseiidae. Great efforts have been established by means of recovering morphological, molecular, and phylogenetic traits for species identification. Traditional identification still relies on external diagnostic characters, which are limited and usually exhibit large phenotypic plasticity within the species, rendering them useless for species delimitation and identification. We decided to increase the number of sequences of the Acari mitochondrial COI (Cytochrome C oxidase I) marker and ITS nuclear ribosomal DNA region for species identification in Tetranychidae and Phytoseiidae. The molecular data allow us to establish species boundaries and phylogenetic relationships among several clades of Acari, mainly Tetranychidae and Phytoseiidae. Sequence comparisons between complete COI and the Acari mitochondrial COI, ITS1-5,8S-ITS2, and ITS2 among all Acari sequences have demonstrated that the selected regions, even small, gave enough informative positions for both species’ identification and phylogenetic studies. Analyses of both DNA regions have unveiled their use as species identification characters, with special emphasis on Acari mitochondrial COI for Tetranychidae and Phytoseiidae species in comparison with the Folmer fragment, which has been universally used as a barcode marker. We demonstrated that the Acari mitochondrial COI region is also a suitable marker to establish a barcode dataset for Acari identification. Our phylogenetic analyses are congruent with other recent works, showing that Acari is a monophyletic group, of which Astigmata, Ixodida, Mesostigmata, Oribatida, and Prostigmata are also monophyletic.

show abstract

Molecular identification of four Panonychus species (Acari: Tetranychidae) in Korea, including new records of P. caglei and P. mori

Cited by 4 publications

References 34 publications

Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods

Species identification of spider mites (Tetranychidae: Tetranychinae): a review of methods

Investigating species boundaries using DNA and morphology in the mite Tyrophagus curvipenis (Acari: Acaridae), an emerging invasive pest, with a molecular phylogeny of the genus Tyrophagus

DNA Barcoding and Phylogeny of Acari Species Based on ITS and COI Markers

Contact Info

Product

Resources

About