Molecular Identification of Ancylostoma caninum Isolated from Cats in Southern China Based on Complete ITS Sequence

The prevalence of intestinal parasites in cats from China was largely unknown prior to this study. The aim of the present study was to investigate the presence of intestinal parasites in cats from central China and also identify risk factors for parasitism. Fecal samples from 360 cats were examined using sugar flotation procedure and fecal smear test by microscope. Cats had mixed two or three kinds of parasites infections. Of the 360 cats feces, intestinal parasites positive feces were 149 (41.39%). 64 (17.78%) were infected with Toxocara cati, 61 (16.94%) with Isospora felis, 41 (11.39%) with Isospora rivolta, 33 (9.17%) with Paragonimus, 23 (6.39%) with hookworms, 11 (3.06%) with Toxoplasma-like oocysts, 10 (2.78%) with Trichuris, 4 (1.11%) with lungworm, 2 (0.56%) with Sarcocystis, and 1 (0.28%) with Trematode. The cats' living outdoor was identified as risk factor by statistical analysis. These results provide relevant basic data for assessing the infection of intestinal parasites in cats from central region of China. In conclusion, there was high prevalence of intestinal parasites in cats from China.

Section: Discussionmentioning

confidence: 55%

Prevalence and Risk Factors of Intestinal Parasites in Cats from China

Yang

Hongde

2015

“…The complete ITS was amplified using a forward primer CAF (5′-GACTGCGGACT GCTGTAT-3′) and a reverse primer CAR (5′-AAGTTCAGCGGGTAGTCA-3′) [11] that were derived from A. ceylanicum (GenBank: JQ812694, AJ920347, AM039739) and A. ceylanicum (GenBank: DQ381541, DQ780009). The PCR amplification was performed in 25 μ L volume containing 17.3 μ L of distilled water, 2.5 μ L of 10 × PCR Buffer, 2 μ L of diethylnitrophenyl thiophosphate (dNTP, 2.5 mM each), 0.5 μ L of each primer (CAF/CAR, 50 pmol/uL), 0.2 μ L of Ex-Taq polymerase (5 U/ μ L), and 2 μ L of the DNA sample.…”

Section: Methodsmentioning

confidence: 99%

“…There were also reports of human infection with A. ceylanicum in Taiwan [8] and Fujian [9]. In Guangzhou, the infection rate of A. ceylanicum in stray dogs and cats was 42.67% [10] and 40.2% [11], respectively. Considering the zoonotic potential of A. ceylanicum infection between animals and humans, it is very important to evaluate the zoonotic risk of A. ceylanicum .…”

Section: Introductionmentioning

confidence: 99%

The Zoonotic Risk ofAncylostoma ceylanicumIsolated from Stray Dogs and Cats in Guangzhou, South China

Liu

Gong

Alsarakibi

et al. 2014

Self Cite

Canine and feline hookworm infection is endemic in many countries with zoonotic transmission representing a potentially significant public health concern. However, there is limited data available on the zoonotic transmission of canine and feline hookworms in China. This study was conducted to evaluate the zoonotic risk of Ancylostoma ceylanicum isolated from stray dogs and cats in Guangzhou, south China. Primer pairs CAF/CAR were designed to amplify complete ITS sequences of obtained A. ceylanicum. The results were compared with fourteen ITS reference sequences of human-derived A. ceylanicum registered in GenBank, and phylogenetic trees were established by using NJ and ML methods. The sequence similarity of three dog-derived and five cat-derived A. ceylanicum with fourteen human-derived A. ceylanicum were 96.8%~100% and 97.8%~100%, respectively. Phylogenetic analysis placed A. ceylanicum isolated from dogs and cats in the same group with A. ceylanicum human isolates. Due to the ability of A. ceylanicum to cause a patent infection in humans, the zoonotic risk arising from dog and cat reservoirs to communities in this region should be determined.

“…To date, among the eight assemblages of G. lamblia found, only assemblages A and B are associated with human infections but are also recovered from a broad range of hosts, including dogs and cats [ 10 ]. Recent epidemiological surveys in China show that the G. lamblia zoonotic assemblage infected dogs and cats was assemblage A, and assemblage B was not found [ 11 , 12 ], while the aetiological agents of hookworm infections were A. ceylanicum and A. caninum [ 13 , 14 ]. Dogs and cats (pet animals) are often infected with these three zoonotic parasites, thus posing great potential risk to public health.…”

Section: Introductionmentioning

confidence: 99%

A Multiplex PCR for Simultaneous Detection of Three Zoonotic ParasitesAncylostoma ceylanicum,A. caninum,andGiardia lambliaAssemblage A

et al. 2015

Self Cite

Ancylostoma ceylanicum, A. caninum, and Giardia lamblia assemblage A are common intestinal parasites of dogs and cats; they can also infect humans, causing parasitic zoonoses. In this study, a multiplex PCR method was developed for simultaneous identification and detection of those three zoonotic parasites. Three pairs of specific primers were designed based on ITS sequence of A. ceylanicum and A. caninum and TPI gene of G. lamblia available in the GenBank. The multiplex PCR reaction system was established by optimizing the reaction condition, and a series of tests on the sensitivity, specificity, and clinical application were also conducted. Results showed that three target fragments were amplified specifically; the detection limit was 10 eggs for both A. ceylanicum and A. caninum, 72 pg DNA for G. lamblia. Of 112 clinical fecal samples, 34.8% and 17.8% samples were positive for A. caninum and A. ceylanicum, respectively, while only 2.7% samples were positive for G. lamblia assemblage A. It is concluded that the established multiplex PCR assay is a convenient, rapid, cost-effective, and high-efficiency method for molecular detection and epidemiological investigation of three zoonotic parasites.