Molecular Identification of Ehrlichia Species and ‘Candidatus Neoehrlichia mikurensis’ from Ticks and Wild Rodents in Shizuoka and Nagano Prefectures, Japan

Naitou, Hirotaka; Kawaguchi, Daizoh; Nishimura, Yuusaku; Inayoshi, Megumi; Kawamori, Fumihiko; Masuzawa, Toshiyuki; Hiroi, Midori; Kurashige, Hideaki; Kawabata, Hiroki; Fujita, Hiromi; Ohashi, Norio

doi:10.1111/j.1348-0421.2006.tb03769.x

Cited by 34 publications

(33 citation statements)

References 21 publications

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“…The morphological and molecular characterization of that isolate, in conjunction with earlier closely related GenBank sequences, supported a novel genetic cluster within the family Anaplasmataceae, and "Candidatus Neoehrlichia mikurensis" was proposed for all current organisms in this group (7). Between 2004 and 2009, this novel bacterium was found in wild rodents in Italy (2), in ticks in Slovakia (13) and the Omsk area of Russia (12), and in ticks and wild rodents in Japan (9). In 2007, the molecular screening of 62 febrile-human samples in Japan did not detect "Candidatus Neoehrlichia mikurensis" DNA (14).…”

Section: Case Reportsupporting

confidence: 53%

“Candidatus Neoehrlichia mikurensis” Infection in a Dog from Germany

Diniz

Schulz²,

Hartmann³

et al. 2011

J Clin Microbiol

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Section: Case Reportsupporting

confidence: 53%

“Candidatus Neoehrlichia mikurensis” Infection in a Dog from Germany

Diniz

Schulz²,

Hartmann³

et al. 2011

J Clin Microbiol

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“…Recently, Anaplasma phagocytophilum infection and coinfection with Rickettsia japonica in humans have been confirmed in central to western parts of Japan (3,4), where infected ticks were present (5). In the past decade, tick-associated Rickettsiales bacteria have also been identified in wild mammals, including deer, boar, Japanese serows, field mice, and wild rats in Japan (2,(6)(7)(8)(9)(10)(11)(12)(13)(14). In particular, wild deer are thought to be one of the most likely reservoirs and/or wide-spreading hosts for tick-associated Rickettsiales bacteria.…”

mentioning

confidence: 99%

A Molecular and Serological Survey of Rickettsiales Bacteria in Wild Sika Deer (Cervus nippon nippon) in Shizuoka Prefecture, Japan: High Prevalence of Anaplasma Species

Wuritu

Yoshikawa

et al. 2015

Jpn J Infect Dis

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SUMMARY:We surveyed Rickettsiales bacteria, including Rickettsia, Ehrlichia, Anaplasma, and Neoehrlichia, in wild sika deer (Cervus nippon nippon) from Shizuoka prefecture, Japan. In spleen samples from 187 deer, Anaplasma phagocytophilum (deer type), A. bovis, and A. centrale were successfully detected by PCR assay targeting to 16S rDNA or p44/msp2, and their positive rates were 96.3z (180/187), 53.5z (100/187), and 78.1z (146/187), respectively. Additionally, 2 or 3 Anaplasma species could be detected from a single deer in 165 spleen samples (88.2z), indicating dual or triple infection. In contrast, A. phagocytophilum (human type) 16S rDNA, Rickettsia gltA, Ehrlichia p28/omp-1, and Neoehrlichia 16S rDNA could not be amplified. The serological test of 105 deer serum samples by immunofluorescence assay showed that the detection of antibodies against antigens of A. phagocytophilum HZ (US-human isolate) and Rickettsia japonica YH were 29.5z (31/105) and 75.2z (79/105), respectively. These findings suggest that A. phagocytophilum (deer type), A. centrale, and A. bovis are highly dominant and prevalent in wild sika deer from Shizuoka, a central region of Japan, and that the antibodies against some Rickettsiales bacteria have also been retained in deer blood.

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“…In addition, "Ca. Neoehrlichia mikurensis" was found in rodent populations in China (17), Japan (18), Sweden (19), and the Netherlands (6). Moreover, an infection was described in a dog in Germany with symptoms similar to ehrlichiosis (20).…”

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Close Geographic Association of Human Neoehrlichiosis and Tick Populations Carrying “Candidatus Neoehrlichia mikurensis” in Eastern Switzerland

et al. 2013

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e Neoehrlichiosis caused by "Candidatus Neoehrlichia mikurensis" is an emerging zoonotic disease. In total, six patients have been described in Europe, with the first case detected in 2007. In addition, seven patients from China were described in a report published in October 2012. In 2009, we diagnosed the first human case of "Ca. Neoehrlichia mikurensis" infection in the Zurich area (Switzerland). Here, we report two additional human cases from the same region, which were identified by broad-range 16S rRNA gene PCR. Both patients were immunocompromised and presented with similar clinical syndromes, including fever, malaise, and weight loss. A diagnostic multiplex real-time PCR was developed for specific detection of "Ca. Neoehrlichia mikurensis" infections. The assay is based on the signature sequence of a 280-bp fragment of the "Ca. Neoehrlichia mikurensis" 16S rRNA gene and incorporates a "Ca. Neoehrlichia mikurensis" species, a "Ca. Neoehrlichia" genus, and an Anaplasmataceae family probe for simultaneous screening. The analytical sensitivity was determined to be below five copies of the "Ca. Neoehrlichia mikurensis" 16S rRNA gene. Our results show that the assay is suitable for the direct detection of "Ca. Neoehrlichia mikurensis" DNA in clinical samples from, for example, blood and bone marrow. In addition, it allows for monitoring treatment response during antibiotic therapy. Using the same assay, DNA extracts from 1,916 ticks collected in four forests in close proximity to the patients' residences (<3 km) were screened. At all sampling sites, the minimal prevalence of "Ca. Neoehrlichia mikurensis" was between 3.5 to 8% in pools of either nymphs, males, or females, showing a strong geographic association between the three patients and the assumed vector.

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Molecular Identification of Ehrlichia Species and ‘Candidatus Neoehrlichia mikurensis’ from Ticks and Wild Rodents in Shizuoka and Nagano Prefectures, Japan

Cited by 34 publications

References 21 publications

“Candidatus Neoehrlichia mikurensis” Infection in a Dog from Germany

“Candidatus Neoehrlichia mikurensis” Infection in a Dog from Germany

A Molecular and Serological Survey of <i>Rickettsiales</i> Bacteria in Wild Sika Deer (<i>Cervus nippon nippon</i>) in Shizuoka Prefecture, Japan: High Prevalence of <i>Anaplasma</i> Species

Close Geographic Association of Human Neoehrlichiosis and Tick Populations Carrying “Candidatus Neoehrlichia mikurensis” in Eastern Switzerland

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