Molecular identification of Meloidogyne species isolated from potato in China and evaluation of the response of potato genotypes to these isolates

Abstract

SummaryRoot-knot nematodes (Meloidogyne spp.) are one of the most economically important groups of plant-parasitic nematodes on potato (Solanum tuberosum), but there has been little study regarding their occurrence on potato in China. In this study, the nematodes in two nematode-infected potato samples collected from Yunnan Province were identified as Meloidogyne spp. Amplicon sizes with species-specific PCR primers indicated that one sample was M. javanica and the other was M. incognita. Cultures established from these two isolates were used to screen 28 potato genotypes in China. All genotypes were susceptible to both M. javanica and M. incognita. However, the mean root galling index and number of egg masses were generally higher for M. javanica than M. incognita. Variable susceptibility was demonstrated among the tested potato genotypes. No available resistant potato cultivars plus a rapid spread of Meloidogyne spp. will result in a potential threat to potato production in China.

Current occurrence and prevalence of root-knot nematodes species, Meloidogyne spp. Goeldi, 1892 (Tylenchida: Meloidogynidae) in ware potato fields of Turkey

“…Potato is one of the main hosts of Meloidogyne spp., and the prevalence and damage of these species have been increasing in recent decades. The damage of RKN to potatoes has been determined in all continents except Antarctica, including the major potato producing countries including China (Mao et al, 2019), India (Singh & Kumar, 2015), the USA (Golden et al, 1980), Germany (Müller et al, 1996), France (Djian-Caporalino, 2012), Holland (Keidel et al, 2007), South Africa (Fourie et al, 2001), and Australia (Nobbs et al, 2001). In potato production, M. arenaria, Meloidogyne enterolobii Yang & Eisenback, 1983, M. incognita and M. javanica and are present in warmer climates, while M. chitwoodi, M. fallax, M. hapla and Meloidogyne minor Karssen et al, 2004 are encountered in relatively colder climates (Nyczepir et al, 1982;Nobbs et al, 2001;Thoden et al, 2012;Onkendi & Moleleki, 2013;Medina et al, 2017).…”

Transgressive resistance to Heterodera glycines in chromosome segment substitution lines derived from susceptible soybean parents

“…Success has been achieved by evaluating RKN reproduction with eggs or egg masses per gram plant root (Atamian et al., 2012), for example, in pepper ( Capsicum annuum L. var. annuum ) (Kiewnick et al., 2009), tomato ( Solanum lycopersicum L.) (Huang et al., 2004), cotton (Wang et al., 2006), rice (Galeng‐Lawilao et al., 2018), potato ( Solanum tuberosum L.) (Mao et al., 2019), soybean (Li et al., 2018), and others. Further, the different combinations of these minor QTL produced wide variation in phenotype among CSSLs, which directly confirms the presence of multiple minor QTL in the soybean accessions through GWAS or biparental population analysis, even in the susceptible genotype and also indirectly explains the wide range of phenotypic variation among soybean accessions carrying the same major resistance genes rhg1 and Rhg4 (Patil et al., 2019).…”