Molecular identification of mung bean accessions (Vigna radiata L.) from Northeast China using capillary electrophoresis with fluorescence‐labeled SSR markers

Abstract

The purpose of this study was to construct the DNA fingerprinting and query system with which to identify mung bean varieties from Northeast China. Eight primer pairs for fluorescent‐labeled simple sequence repeat markers with high polymorphism and a strong discrimination ability were used to detect 151 mung bean varieties from Northeast China. A total of 59 bands were detected, ranging from 4 to 11 with an average of 7.4. An unweighted pair group method analysis was used to cluster the mung bean varieties into five separate groups according to their genetic characteristics, which were consistent with the results of principal component analysis. Unique identification cards and barcodes with which each variety can be identified were established according to fingerprinting. A query system was also established which can provide the information and genetic relationships among some indefinite variety and mung bean varieties from Northeast China. This research will significantly assist in identification, traceability management, protection of origin, and intellectual property rights. It will also provide essential technical support for revealing the level of genetic diversity for breeding and germplasm innovation of mung bean varieties in Northeast China.

Efficient Fingerprinting of the Tetraploid Salix psammophila Using SSR Markers

“…Normally, a single pair of markers cannot differentiate each of the sampled individuals [28]. In this study, 22 SSR markers were highly polymorphic in 261 accessions of S. psammophila with an average number of alleles (A) of 10.41 and average number of four allele genotypes (G) for each locus of 68.36, which was much greater than the values shown in a few former studies of diploid plants (Robinia pseudoacacia L. [29] and Vigna radiata L. [30]. ), implying that the abundance of genotypes in tetraploids is helpful to efficiently reconstruct the fingerprints.…”

“…PIC plays a crucial role in the assessment of availability of SSR markers which reflect polymorphism of markers. The average value of PIC was 0.63, which was higher than related estimates in other plant samples [29][30][31]. The higher polymorphism for tetraploid plants utilized to score alleles benefits from the clear resolution of the capillary DNA fragments [32], and the TP-M13-SSR not only avoided amplicons overlapping but also provided an economic and effort saving method for microsatellite genotyping [18].…”

“…In order to solve the problems of low identification rates of the single marker, some researchers tried to use the method of combined markers [35,36]. The number of markers have a big impact on the ability of the marker combinations identification, and some markers with sufficient informativeness remarkably enhance the identification rates of the marker combinations [30]. In our study, a genetic toolkit for S. psammophila identification consisting of nine-marker combination (G+I+J+N+O+Q+S+T+U) was selected, which could thoroughly separate each of the 261 accessions based on their special fingerprinting profiles.…”

“…In this study, nine-marker combination (G+I+J+N+O+Q+S+T+U) could distinguish the 261 accessions from one another, which was much less than the number of markers shown in a few earlier studies of diploid plants. For example, Zhao et al [30] constructed the fingerprints based on eight SSR markers, which could identify 151 mung bean varieties; a total of 110 cultivars of Chinese black locust were distinguished by eight pairs of EST-SSR and SSR primers [29].…”

“…These DNA barcodes can be identified rapidly and conveniently when scanned by computer. This system took the place of recording the size of alleles by electrophoregrams and helped to avoid mistakes by eye and the influence of the surrounding environment [30]. Therefore, this study outlines a potentially efficient approach for germplasm resource management and intellectual property right protection of S. psammophila.…”