Molecular identification of new circulating Hyalomma asiaticum asiaticum from sheep and goats in Duhok governorate, Iraq

Ismael, Shameeran Salman; Omer, Lokman Tayib

doi:10.33899/ijvs.2020.126330.1298

Cited by 9 publications

(12 citation statements)

References 25 publications

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“…This study was conducted in an area with high tick endemicity. There are about 878 tick species in Iraq [ 15 ] that play a significant role in the dissemination of various infectious illnesses in animals and humans [ 10 , 16 ]. Bovine hemotropic Mycoplasma widely distributes Rickettsial diseases and their associated risk factors [ 13 ].…”

Section: Discussionmentioning

confidence: 99%

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

2022

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Background and Aim: Hemotropic Mycoplasmas are small epierythrocytic bacteria that cause infectious anemia in several livestock species and in humans. Several reports have been made on hemoplasma infections in the south and north of Iraq, but there have been no studies in the middle Euphrates of Iraq. This study aimed to evaluate the presence of hemoplasma species in cattle in Al-Qadisiyah Province, Iraq. Materials and Methods: Two hundred blood samples were collected from cattle with pale mucous membrane from regions with heavy tick endemicity. The samples were analyzed for the presence of Rickettsia pathogens using thin blood smears and the Diff-Quik stains. All the samples were also examined using polymerase chain reaction (PCR) to amplify the 16S ribosomal ribonucleic acid (rRNA) gene to confirm the presence of the smear-identified microorganisms. Ten PCR positive samples were subjected to 16S rRNA partial gene sequencing to identify the species. Results: The findings uncovered positivity in 68 (34%) blood smears. PCR revealed positive confirmation in 18 (9%) of the 200 blood samples. Mycoplasma wenyonii and Candidatus mycoplasma hemobos were identified from 10 PCR positive samples. The nucleotide sequences of the isolates were closely related to isolates from cattle, buffalo, and dogs in Vietnam, Cuba, India, and Germany. Conclusion: Bovine hemoplasma infections are prevalent in cattle in the Al-Qadisiyah Province in Iraq. Our results may have significance for the development of control programs.

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Section: Discussionmentioning

confidence: 99%

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

2022

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“…The worms were placed in a slightly hot physiological salt solution, and the morphology of these worms was identified using a light microscope. Some portions of tapeworms (mature segments) were fixed in 70% ethanol and stained with carmine stain and mounted in Canada balsam (5), and another portion of tapeworms were kept at -20°C for molecular study.…”

Section: Collection Of Samplesmentioning

confidence: 99%

“…Sheep is considered the most crucial preferable livestock for human consumption in an Arab country (1)(2)(3)(4)(5). The genus Moniezia belongs to the Family Anopocephalidae.…”

Section: Introductionmentioning

confidence: 99%

Morphometric and molecular characterization of Moniezia species in sheep in Mosul city, Iraq

Suleiman

Alhayali

Al-Taee

2022

IJVS

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The current study examined 100 small intestines collected randomly from sheep slaughtered in the abattoir and butcher's shops from different Mosul city / Iraq areas of both sexes (55 females, 45 males) and different ages. Moniezia expansa was diagnosed in 9 samples of intestines by studying the morphometric characteristics of these tapeworms, especially the mature segments, in which both the ovaries and vitelline glands appeared in the ring shape on either side of the body segments and the rosette-like shape of the interproglotidial glands. No significant difference was noticed between males and females of sheep in our study, and the infection rate was 10% in sheep less than a year old and older than two years, with no significant difference between the age groups. The results of the molecular analysis by using conventional polymerase chain reaction technique confirmed the diagnosis of these worms, which belong to the genus Moniezia, with a product reaction of 700 base pairs. The sequencing result shows two strains of Moniezia expanza, which isolated from Iraq (Moniezia expansa-Iraqi one and Moniezia expansa-Iraqi 2) were similar to each other had a significant distance to other strains. The study also showed that Moniezia expansa is different from the same species in other countries.

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“…Following PCR amplification, the 1462-bp amplification product was loaded on a 1% agarose gel and stained with ethidium bromide (17). The electrophoresis was run at an electric current of 85 Volt for 65 min, and a UV transilluminator projected them (Figure 1).…”

Section: Gel Electrophoresismentioning

confidence: 99%

Molecular characterization and phylogenic analysis of Anaplasma spp. in small ruminants from Sulaymaniyah governorate, Iraq

Abdullah¹,

Dyary²

2022

IJVS

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Anaplasma spp. are significant arthropod-borne bacteria globally, but documented information about anaplasmosis in small ruminants in the north of Iraq is insufficient. Hence, this study was conducted to determine the prevalence of Anaplasma spp. and identify sheep and goat tick vector populations in Sulaymaniyah Governorate, north Iraq. The study population consisted of 470 sheep and 145 goats from 45 livestock farms in 10 geographical locations of Sulaymaniyah Governorate. The study was accomplished from April to December 2017. Blood samples were taken from the jugular vein and used for DNA extraction. Polymerase chain reaction (PCR) was conducted using primers based on the 16S rRNA of Anaplasma spp. Fragments of PCR products were sequenced for phylogenetic analysis. The prevalence of Anaplasma spp. was 58.9% based on the PCR results. Furthermore, 58.9% of sheep and 57.9% of goats were positive for anaplasmosis. The sequences represented 100% identity with previously documented GenBank isolates of A. ovis from Iran, the Netherlands, China, and Mongolia. Altogether, 150 Ixodid ticks were picked from small ruminants within the same flocks and were identified based on morphological features. Various infestation rates were observed; about 40% of the Ixodid ticks belonged to Rhipicephalus sanguineus, 34% belonged to Rhipicephalus turanicus, 18% were Hyalomma anatolicum, and 8% were Boophilus microplus (Rhipicephalus microplus). The present report is the first molecular study of Anaplasma species in small ruminants from Sulaymaniyah Governorate in northern Iraq to the best of our knowledge. The study concluded that anaplasmosis was endemic in small ruminants from the investigated areas.

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Molecular identification of new circulating Hyalomma asiaticum asiaticum from sheep and goats in Duhok governorate, Iraq

Cited by 9 publications

References 25 publications

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

First study on microscopic and molecular evidences of two bovine hemoplasma species in cattle herds in Al-Qadisiyah Province, Iraq

Morphometric and molecular characterization of Moniezia species in sheep in Mosul city, Iraq

Molecular characterization and phylogenic analysis of Anaplasma spp. in small ruminants from Sulaymaniyah governorate, Iraq

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