Molecular identification of new varieties ofNierembergia linariaefolia (Graham), a native Argentinean ornamental plant

Escandón, Alejandro Salvio; Zelener, Noga; Torre, Mariana Pérez de la; Soto, S.

doi:10.1007/bf03194668

Cited by 8 publications

(6 citation statements)

References 30 publications

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“…The higher values of Rp belonged to the 15 and 20 base primers (5 and 3.8, respectively) (Table 1), indicating a better distinction of the varieties. So, these results also confirm the utility of the Rp as measure of capacity of a primer to discriminate among closely related individuals as was pointed out by Prevost and Wilkinson (1999) and Escandón et al (2007).…”

Section: Resultssupporting

confidence: 73%

Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

Piña-Escutia¹,

Vázquez-García²,

Arzate-Fernández³

2010

Electron. J. Biotechnol.

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Section: Resultssupporting

confidence: 73%

Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

Piña-Escutia¹,

Vázquez-García²,

Arzate-Fernández³

2010

Electron. J. Biotechnol.

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“…Inter-simple sequence repeats (ISSRs) are microsatellite molecular markers first described by Zietkiewicz et al (1994) that are now applied in genetic map construction (Bornet and Branchard, 2001;Sankar and Moore, 2001), gene mapping (Ratnaparkhe et al, 1998), genetic diversity analysis (Luan et al, 2006), and identification of germplasm resources (Escandón et al, 2007). ISSR molecular markers have been applied successfully in studies of the genetic diversity and genetic structure of endangered tree species such as Ginkgo biloba (Ge et al, 2003), Camellia nitidissima (Bin et al, 2005), and Paeonia delavayi (Yang et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity of natural populations of Machilus thunbergii, an endangered tree species in eastern China, determined with ISSR analysis

Liu¹,

Sun²,

Jiang³

et al. 2013

Genet. Mol. Res.

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ABSTRACT. The genetic diversity of 10 Machilus thunbergii populations in eastern China was analyzed using inter-simple sequence repeat markers. The populations showed high genetic diversity, with an overall population genetic diversity of 0.2343. Genetic diversity varied largely among populations, and populations with the highest genetic diversity were mainly from the eastern and western parts of the natural distribution area. Small populations, lack of effective gene flow, and fragmentation of habitats have led to greater genetic differentiation among populations, with 41.18% of genetic variation existing among populations. Unweighted pair-group method with arithmetic mean cluster analysis indicated that populations distributed between latitudes 25° and 31°N were clustered together and should be prioritized for in situ conservation. Northern, eastern, and southern populations were located in peripheral areas of the distribution range and were clustered separately. Collection of distinctive germplasm from peripheral populations should be promoted and ex situ conservation of elite germplasm should be implemented.

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“…In this work, we used the same primers reported by Escandón et al (2007), who identified six new varieties of Nierembergia linariaefolia. In that work, the six varieties revealed 443 polymorphic loci, with a 96.39% of polymorphism and a mean of 32.80 loci per primer.…”

Section: Discussionmentioning

confidence: 99%

“…Furthermore, it is essential to have an unequivocal identification method to verify the material obtained. In this context, the ISSRs are a valuable tool to analyze the genetic variability in a plant collection or wild species, and are useful to identify genotypes, even in highly related individuals (González et al 2002;Pérez de la Torre et al 2003;Hundsdoerfer and Wink, 2005; Pérez de la Torre and Escandón, 2006;Escandón et al 2007;Martins-Lopes et al 2007;Christopoulos et al 2010;Pérez de la Torre et al 2010). In this context, the ISSRs are a valuable tool to analyze the genetic variability in a plant collection or wild species, and are useful to identify genotypes, even in highly related individuals (González et al 2002;Pérez de la Torre et al 2003;Hundsdoerfer and Wink, 2005; Pérez de la Torre and Escandón, 2006;Escandón et al 2007;Martins-Lopes et al 2007;Christopoulos et al 2010;Pérez de la Torre et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Analysis of genetic variability by ISSR markers in Calibrachoa caesia

Torre¹,

García²,

Heinz³

et al. 2012

Electron. J. Biotechnol.

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Background: Calibrachoa Cerv. (ex La Llave & Lexarza) is a genus of the Solanaceae family (La Llave and Lexarza, 1825). This genus has a high ornamental and economic value due to its intrinsic variability and multiplicity of flower colours. In Argentina there are eight native species, and one of them is Calibrachoa caesia. The genetic diversity among 35 accessions of C. caesia, from five departments in the province of Misiones, was analyzed using ISSR markers. Results: Thirteen ISSR primers yielded a reproducible banding pattern, with 701 amplified loci and 98% of polymorphism. The ISSR primers 5'CT, 5'CA, 5'GA, 5'GACA, 3'CAC, 3'TG and 3'TC generated 100% polymorphic patterns. The Rp values ranged from 23.20 to 10.29 for 5'GACA and 3'AG primers, respectively, while the average values for MI and PIC were 0.367 and 0.231, respectively. The more informative primers were 5'GACA and 5'GA, and the less informative was 3'AC. Simple matching coefficient of similarity varied from 0.8875 to 0.6659, indicating high levels of genetic similarity among the genotypes studied. The UPGMA cluster analysis indicated three distinct clusters; one comprised genotypes of the five departments, while the second included individuals from Guaraní and Oberá regions and the third cluster included the San Pedro individuals. The overall grouping pattern is in agreement with principal coordinate analysis (PCoA). Conclusions: The Bayesian cluster analysis revealed structuring of the C. caesia population and two clusters were identified, which correspond to UPGMA major clades. The AMOVA test for all populations showed highest genetic variation within populations (90%), meanwhile the F st coefficient was 0.098, indicating a medium differentiation between populations. These results showed a great intrapopulation genetic diversity but no significant difference was detected among populations. In this work the use of thirteen ISSR markers, allowed the characterization of every individual examined. Therefore, the possibility of using molecular data for varietal identification is a promising tool for application in future breeding programs in the genus Calibrachoa.Note: Electronic Journal of Biotechnology is not responsible if on-line references cited on manuscripts are not available any more after the date of publication. Supported by UNESCO / MIRCEN network.

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Molecular identification of new varieties ofNierembergia linariaefolia (Graham), a native Argentinean ornamental plant

Cited by 8 publications

References 30 publications

Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

Genetic diversity of natural populations of Machilus thunbergii, an endangered tree species in eastern China, determined with ISSR analysis

Analysis of genetic variability by ISSR markers in Calibrachoa caesia

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