Variety discrimination of Tigridia pavonia (L.f.) DC. assesed by different length RAPD primers

Piña-Escutia, José Luis; Vázquez-García, Luis Miguel; Arzate-Fernández, Amaury M.

doi:10.2225/vol13-issue4-fulltext-7

Cited by 3 publications

(2 citation statements)

References 15 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…According to this, it is possible that the GC content favored the major efficiency of the 15-(65% GC) and 20-base primers (60% GC) that were used in this study because more DNA fragments were amplified and also a greater percentage of polymorphism was detected (92.7 and 98.5, respectively) compared with those that were amplified with 10-base primers, which had 72% GC content and 80% polymorphism ( Table 2). Our results are similar to those that were reported by Solouki et al (2008) and Piña-Escutia et al (2010b), where more DNA fragments and 100% polymorphism were obtained using a long primer with low GC content.…”

Section: Rapd Markerssupporting

confidence: 92%

Genetic diversity of Cosmos species revealed by RAPD and ISSR markers

Rodríguez-Bernal

Piña-Escutia²,

Vázquez-García³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. The genus Cosmos is native of America and is constituted by 34 species; 28 of them are endemic of Mexico. The cosmos are used as a nematicide, antimalarial, and antioxidative agent. The aim of this study was to estimate the genetic diversity among 7 cosmos species based on random amplified polymorphic DNA (RAPD) and inter-simple sequences repeats (ISSR) markers. With RAPD markers, the obtained polymorphism was 91.7 % and the genetic diversity was 0.33, whereas these values were 65.6%, and 0.22 from ISSR markers, respectively, indicating the presence of high genetic diversity among the Cosmos species that were analyzed. The unweighted pair group method with arithmetic mean dendrograms that were obtained with both markers were notably similar, revealing 2 clusters and indicating a clear genetic differentiation among the Cosmos species that were assessed. The first cluster comprised the species Cosmos sulphureus, Cosmos pacificus, and Cosmos diversifolius, while the second cluster included the species Cosmos purpureus, Cosmos crithmifolius, Cosmos bipinnatus, and Cosmos parviflorus. Besides this, the Cosmos species were clustered according to their collection sites. The Mantel test corroborates the correlation between the genetic distance and the geographic altitude of each Cosmos species. The results suggest that it is necessary to preserve the Cosmos species in their natural habitat in addition to the germoplasm collection for ex situ conservation.

show abstract

Section: Rapd Markerssupporting

confidence: 92%

Genetic diversity of Cosmos species revealed by RAPD and ISSR markers

Rodríguez-Bernal

Piña-Escutia²,

Vázquez-García³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

show abstract

“…Forty-three accessions from Korea and 23 accessions from China were successfully distinguished from each other using just four of 14 SSR markers, providing a simple approach for discriminating between Korean and Chinese sesame accessions. Piña-Escutia et al (2010) used 10-, 15-, or 20-base primers to discriminate between nine varieties of Tigridia pavonia (L.f.) DC in Mexico. The authors found that a long primer (20 bases) generated a similar dendrogram to those constructed using shorter primers, with the exception of a single variety whose placement shifted as a result of using a longer primer.…”

Section: Discussionmentioning

confidence: 99%

Application of Simple Sequence Repeat (SSR) Markers for the Discrimination of Korean and Chinese Sesame (Sesamum indicum L.) Accessions

Park¹,

Suresh²,

Piao³

et al. 2014

Plant Breed. Biotech.

View full text Add to dashboard Cite

This is an Open-Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/3.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. ) is an ancient oil seed crop that has been referred to as the 'queen of oilseeds' by virtue of its high quality oil. Fourteen simple sequence repeat (SSR) markers were used to differentiate 70 sesame (Sesame indicum L) accessions. Forty-three of the accessions were from Korea and 27 were from China. An initial round of analysis using two SSR markers (GBssr-sa-005 and GBssr-sa-182) identified 21 distinct accessions. A second round of analysis using one additional SSR marker (GBssr-sa-108) identified 25 more distinct accessions. Addition of a fourth SSR marker (GBssr-sa-184) for a third round of analysis identified an additional 20 accessions. This study demonstrates differentiation between 43 accessions from Korea and 23 accessions from China by four SSR markers. Application of Simple Sequence Repeat (SSR) Markers for the

show abstract