Molecular identification of Phortica variegata and Phortica semivirgo (Drosophilidae, Steganinae) by PCR-RFLP of the mitochondrial cytochrome oxidase c subunit I gene

Cantacessi, Cinzia; Traversa, Donato; Testini, Gabriella; Lia, Riccardo P.; Cafarchia, Claudia; Máca, Jan; Otranto, Domenico

doi:10.1007/s00436-008-1027-z

Cited by 6 publications

(3 citation statements)

References 13 publications

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“…However, while P. variegata is the main vector candidate for T. callipaeda in European countries, others species like Phortica semivirgo Máca, 1977 are thought to play a role in the transmission of this eye worm [ 24 ]. Furthermore, owing to the similar taxonomic characters of adult worms of P. variegata and P. semivirgo , identification based only on morphology is difficult and requires specific technical skills (reviewed in [ 25 ]).…”

Section: Introductionmentioning

confidence: 99%

Detection of Thelazia callipaeda in Phortica variegata and spread of canine thelaziosis to new areas in Spain

et al. 2018

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BackgroundThe fruit fly Phortica variegata (Drosophilidae: Steganinae) feeds on the ocular secretions of animals and humans, and has been described as an intermediate host of the eye worm Thelazia callipaeda (Spirurida: Thelaziidae) in Italy. Despite the increased detection of T. callipaeda in many European countries, information about its vector role in natural conditions is still limited. In the Iberian Peninsula, thelaziosis caused by T. callipaeda has been reported in dogs, cats, red foxes, wild rabbits and humans.MethodsIn the last seven years, we have detected increased numbers of cases of canine thelaziosis at three locations in mainland Spain: Site 1, La Vera region (Cáceres Province, central-western Spain; 51 cases); Site 2, El Escorial municipality (Madrid Community, central Spain; 23 cases); and Site 3, Miraflores de la Sierra municipality (Madrid Community, central Spain; 41 cases). Site 1 is considered endemic for T. callipaeda while the other two sites have been recently recognised as risk zones for T. callipaeda infection.ResultsFrom June 2016 to September 2017, 2162 flies were collected and morphologically identified as Phortica spp. (Site 1, n = 395; Site 2, n = 1544; and Site 3, n = 223). Upon dissection, third-stage T. callipaeda larvae were found in two out of 155 flies examined from Site 1, and both these larvae tested molecularly positive for the eye worm. Of the 395 flies collected from Site 1, 371 were molecularly processed for arthropod species identification and T. callipaeda detection. All 371 flies were identified as P. variegata and 28 (7.5%; 95% CI: 4.8–10%) tested positive for T. callipaeda DNA haplotype 1.ConclusionsOur findings indicate that T. callipaeda circulates among dogs and P. variegata in Spain, where zoonotic cases have been also reported. The co-existence of canine thelaziosis and Phortica spp. in geographical areas previously considered free of the eye worm indicates a risk of infection for both animals and humans living in this region.

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Section: Introductionmentioning

confidence: 99%

Detection of Thelazia callipaeda in Phortica variegata and spread of canine thelaziosis to new areas in Spain

et al. 2018

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“…For example, Enterobacter sp. strongly attracted Bactrocera tau adults according to laboratory and field bioassays [ 10 ]. Citrobacter species were found to exhibit the greatest attraction to Bactrocera dorsalis [ 11 ].…”

Section: Introductionmentioning

confidence: 99%

The species and abundance of gut bacteria both positively impact Phortica okadai behavior

Li,

Wang,

Wang

et al. 2024

Parasites Vectors

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Background Gut bacteria, which serve as essential modulators, exert a significant impact on insect physiology and behavior and have substantial application potential in pest management. The dynamics of gut bacteria and their impact on Phortica okadai behavior remain unclear. Methods In this study, the dynamics of gut bacteria at different developmental stages in P. okadai were analyzed using 16S ribosomal RNA (rRNA) gene sequencing, and the species and abundance of gut bacteria that affect host behavior were examined via behavioral experiments. Results A total of 19 phyla, 29 classes, 74 orders, 101 species, and 169 genera were identified. The results of the behavioral experiments indicated that the species Lactiplantibacillus argentoratensis, Acetobacter tropicalis, Leuconostoc citreum, and Levilactobacillus brevis effectively influenced the feeding preference of P. okadai, and the single-bacterium-seeded P. okadai exhibited feeding preferences distinct from those of the germ-free (GF) and wild-type P. okadai. Conclusions The species and relative abundance of gut bacteria together positively impact P. okadai behavior. Lactiplantibacillus argentoratensis, as the most attractive bacteria to P. okadai, presents opportunities for novel pest control strategies targeting this vector and agricultural pest. Graphical Abstract

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“…For example, Enterobacter sp. strongly attract Bactrocera tau adults via laboratory and eld bioassays [10]. Citrobacter sp.…”

Section: Introductionmentioning

confidence: 99%

The species and abundance of gut bacteria jointly mediate a positive impact on Phortica okadai behavior

Li,

Wang,

Wang

et al. 2024

Preprint

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Background Gut bacteria, serving as essential modulators, exert a significant impact on insect physiology and behavior, holding substantial potential for application in pest management. The dynamics of gut bacteria and the impact on Phortica okadai behavior remain unclear. Methods In this study, the dynamics of gut bacterial at different developmental stages in P. okadai was analyzed using 16S rRNA gene sequencing and the species and abundance of gut bacteria that affect host behavior were examined by behavioral experiments. Results As a result, a total of 19 phyla, 29 classes, 74 orders, 101 species, and 169 genera were identified. The results of the behavioral experiments indicated that the species, including Lactiplantibacillus argentoratensis, Acetobacter tropicalis, Leuconostoc citreum, and Levilactobacillus brevis, exhibited an effective influence on the feeding preference of P. okadai and the single-bacteria-seeded P. okadai exhibited feeding preferences distinct from germ-free and wild-type. Conclusions Overall, this study not only demonstrates the dynamics of gut bacteria in P. okadai but, significantly, also posits novel evidence. It suggests that beyond the gut bacteria species, the abundance also positively influences host behavior. These findings contribute to the development of novel pest control strategies for preventing agricultural pests and vectors.

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Molecular identification of Phortica variegata and Phortica semivirgo (Drosophilidae, Steganinae) by PCR-RFLP of the mitochondrial cytochrome oxidase c subunit I gene

Cited by 6 publications

References 13 publications

Detection of Thelazia callipaeda in Phortica variegata and spread of canine thelaziosis to new areas in Spain

Detection of Thelazia callipaeda in Phortica variegata and spread of canine thelaziosis to new areas in Spain

The species and abundance of gut bacteria both positively impact Phortica okadai behavior

The species and abundance of gut bacteria jointly mediate a positive impact on Phortica okadai behavior

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