Molecular identification of Schisandra chinensis and its allied species using multiplex PCR based on SNPs

Kim, Jung Sung; Jang, Hee-Woon; Kim, Jinsook; Kim, Hyuk-Jin; Kim, Joohwan

doi:10.1007/s13258-011-0201-3

Cited by 14 publications

(9 citation statements)

References 29 publications

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“…Moldenke (何首烏; Ha-su-o in Korean and he shou wu in Chinese). Similarly, the specific molecular marker for S. chinensis, P. ginseng, Tomato, Zea mays were developed (Batley et al, 2002;Deynze et al, 2007;In et al, 2010;Kim et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…For examples, Korean Ginseng (Panax ginseng)-specific primer was developed based on SNP in ITS region . And, Kim et al (2012) used rbcL and ITS regions to devise the specific primer for identification of Schisandra chinensis with other related species.…”

mentioning

confidence: 99%

“…Amplification Refractory Mutation System (ARMS) has been usefully applied with SNP as a molecular genetic technique (Kim et al, 2012). For ARMS, when the target DNA does not match the base in 3' end of primer, there is no extension step by DNA polymerase in PCR progress.…”

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confidence: 99%

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Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites

Park¹,

Yoon²,

Kim³

et al. 2015

Korean Journal of Plant Resources

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-Reynoutria japonica and R. sachalinensis have been used as medicinal resources in Korea. However, it is difficult to identify and determine these medicinal herbs correctly because they are usually customized and purchased as the fragmented rhizomes types. To develop molecular markers for distinguishing two species, we analyzed and compared the chloroplast DNA sequences of seven loci (atpB, matK, ccD-psaI, atpF-H, trnL-trnF, psbK-I and rpl32-trnL). Among them, we found two effective SNPs in psbK-I region for R. japonica and atpF-H region for R. sachalinensis. Based on these SNP sites, we designed the new R. japonica-specific primer which is able to amplify 300 bp fragment in psbK-I region. A similar strategy was applied for the atpF-H region of R. sachalinensis. These molecular markers would be successfully applied to recognize R. japonica and R. sachalinensis.

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Section: Discussionmentioning

confidence: 99%

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confidence: 99%

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Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites

Park¹,

Yoon²,

Kim³

et al. 2015

Korean Journal of Plant Resources

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“…As yet some works had been done to differentiation or identification of Schisandra species using RAPD, ISSR, rbcL and ITS [18][19][20]. Recently, being part of ITS, ITS2 was relatively easy to be amplified using one pair of universal primers [21,22].…”

Section: Introductionmentioning

confidence: 99%

“…Additionally, some limitations in traditional taxonomy prevent this technique from meeting the complicated demands of species recognition [17]. As such, a method for the simple and accurate authentication of Schisandra is indispensible.As yet some works had been done to differentiation or identification of Schisandra species using RAPD, ISSR, rbcL and ITS [18][19][20]. Recently, being part of ITS, ITS2 was relatively easy to be amplified using one pair of universal primers [21,22].…”

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Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2

Bing

Han

et al. 2013

Acta Soc Bot Pol

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IntroductionSchisandra chinensis (Turcz.) Baill. grows mainly in the northeast China and its mature fruits are famous traditional Chinese medicine recorded in "Chinese Pharmacopoeia". As a traditional medicinal herb, S. chinensis has been used as an astringent curing dry cough, asthma, night sweats, nocturnal seminal emissions and chronic diarrhea [1,2]. Modern medical research had proved that S. chinensis contains multiple active components used to protect liver [3][4][5], prevent senility [6][7][8][9], restrain oxidation [10,11], improve human body immunity ability [12], regulate the central nervous system and so on [13,14].Commonly S. chinensis and its adulterants are frequently found in the market together. Schisandra sphenanthera Rehd. et Wils. is important traditional Chinese medicine recorded in "Chinese Pharmacopoeia", too, but its active components of the kinds and levels are clearly different from S. chinensis [15]. Conventional viewpoints are that the medicinal value of S. chinensis is better than S. sphenanthera. But the two medicines are often mistaken for each other [16]. The identification of the two species of Schisandra is difficult when based solely on morphological characteristics. Additionally, some limitations in traditional taxonomy prevent this technique from meeting the complicated demands of species recognition [17]. As such, a method for the simple and accurate authentication of Schisandra is indispensible.As yet some works had been done to differentiation or identification of Schisandra species using RAPD, ISSR, rbcL and ITS [18][19][20]. Recently, being part of ITS, ITS2 was relatively easy to be amplified using one pair of universal primers [21,22]. In addition, ITS2 had been found to provide taxonomic signature in systematic evolution [23,24]. The ITS2 region was also a promising potential molecular marker to be used for rapid taxonomic classification [21,22]. To our best knowledge, applying ITS2 region to identify plant materials from Schisandra with such a large sample size and geographic range had not been reported. And these studies were not found whether the ITS2 region could be used as a genomic marker to identify different Schisandra populations from different ecological environment and geographical distribution. In the current study, we utilized ITS2 as a DNA barcode to distinguish medicinal plants within the Schisandra genus and populations in order to ensure their safe, effective application in traditional using. AbstractTo test whether the internal transcribed spacer 2 (ITS2) region is an effective marker for using in authenticating of the Schisandra chinensis at the species and population levels, separately. And the results showed that the wild populations had higher percentage of individuals that had substitution of C→A at site 86-bp than the cultivated populations. At sites 10-bp, 37-bp, 42-bp and 235-bp, these bases of the Schisandra sphenanthera samples differed from that of S. chinensis. Two species showed higher levels of inter-specific divergence than intra-specif...

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How Far Advanced is the DNA-Based Identification of the BELFRIT-List?

Novak

Ruzicka

Schmiderer

2017

Food Supplements Containing Botanicals: Benefits, Side Effects and Regulatory Aspects

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Molecular identification of Schisandra chinensis and its allied species using multiplex PCR based on SNPs

Cited by 14 publications

References 29 publications

Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites

Molecular Identification of Reynoutria japonica Houtt. and R. sachalinensis (F. Schmidt) Nakai Using SNP Sites

Identification of medicinal plant Schisandra chinensis using a potential DNA barcode ITS2

How Far Advanced is the DNA-Based Identification of the BELFRIT-List?

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