Molecular identification of some African strains of Ralstonia solanacearum from eucalypt and potato

Fouché-Weich, Joanne; Poussier, Stéphane; Trigalet-Demery, Danielle; Berger, Dave K.; Coutinho, Teresa A.

doi:10.1007/s10327-006-0307-7

Cited by 8 publications

(14 citation statements)

References 15 publications

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“…2006). Importantly, although they are both biovar 3, the Eucalyptus isolate BCCF401 from South Africa in this study is distinct from the well‐characterized tomato isolate GM1000 from French Guyana based on PCR‐RFLP and AFLP analysis (Fouché‐Weich et al. 2006).…”

Section: Introductionmentioning

confidence: 70%

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A Eucalyptus bacterial wilt isolate from South Africa is pathogenic on Arabidopsis and manipulates host defences

et al. 2011

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Summary Ralstonia solanacearum, the causal agent of bacterial wilt, has been reported from Eucalyptus plantations in at least three countries in Africa. The lack of genomics resources in Eucalyptus species led us to develop and study a pathosystem between a previously characterized South African isolate and the model plant Arabidopsis thaliana. Ralstonia solanacearum BCCF401 isolated from a Eucalyptus grandis × Eucalyptus camaldulensis hybrid was shown to cause disease on A. thaliana ecotype Col‐5. Arabidopsis genomics tools were exploited to investigate gene expression changes during wilt disease development, and thereby develop hypotheses that can be tested in Eucalyptus once genomics resources are available. Transcriptome analysis using 5000 A. thaliana ESTs was performed and revealed 141 genes that were differentially regulated by R. solanacearum infection (at a significance threshold of p < 0.03; Bonferroni corrected). A software tool ‘Rank Correlation Comparer’ was developed to compare expression profiles with Arabidopsis Affymetrix NASCArray data. High correlations were observed between the response of Arabidopsis plants to both Eucalyptus (BCCF401) and tomato (GMI1000) isolates of R. solanacearum, as well as to Pseudomonas syringae, Botrytis cinerea and treatment with abscisic acid. Basal defence responses in Col‐5 in response to R. solanacearum were investigated by comparing the expression data following R. solanacearum infection to data after treatment with the Pathogen Associated Molecular Patterns (PAMP) flg22 and lipopolysaccharide, and the Type Three Secretion System deficient Pseudomonas syringae pv. tomato hrp− mutant. A subset of the genes which were induced by PAMPs were repressed by R. solanacearum infection, and vice versa, suggesting that these genes may be repressed or induced, respectively, by specific R. solanacearum effectors. We hypothesize that these genes represent targets of R. solanacearum effectors. The pending release of the Eucalyptus genome sequence will enable orthologues to be identified and these hypotheses to be tested in Eucalyptus trees.

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Section: Introductionmentioning

confidence: 70%

“…2000). Subsequently, the same isolate (BCCF401), as well as Eucalyptus isolates from two other African countries (Uganda and Democratic Republic of Congo) were confirmed to be biovar 3 (Fouché‐Weich et al. 2006).…”

Section: Introductionmentioning

confidence: 89%

A Eucalyptus bacterial wilt isolate from South Africa is pathogenic on Arabidopsis and manipulates host defences

et al. 2011

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“…solanacearum BCCF402 is a biovar 3 phylotype I strain from Eucalyptus trees (Fouché-Weich et al 2006). Root inoculations were performed on A. thaliana accessions Be-0, Kil-0, and Nd-1, and plants were monitored for disease development.…”

Section: Resultsmentioning

confidence: 99%

“…solanacearum biovar 3 phylotype I strain BCCF402 (Fouché-Weich et al 2006;Roux et al 2000) was grown on solidified bacto-agar glucose triphenyltetrazolium chloride (BGT) medium at 28°C for 48 h and mucoid colonies were transferred to liquid B medium (Deslandes et al 1998). Rifampicin mutants were prepared as described previously (Naidoo et al 2011).…”

Section: R Solanacearum Strainsmentioning

confidence: 99%

“…We previously reported that R. solanacearum BCCF401, collected from Eucalyptus grandis × E. camaldulensis plantations in South Africa (Fouché-Weich et al 2006), was pathogenic on Arabidopsis accession Col-0 (Naidoo et al 2011). In the current study, we characterized the response of Arabidopsis accessions to another Eucalyptus strain of R. solanacearum called BCCF402.…”

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Gene-for-Gene Tolerance to Bacterial Wilt in Arabidopsis

Linden¹,

Bredenkamp²,

Naidoo³

et al. 2013

MPMI

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Bacterial wilt caused by Ralstonia solanacearum is a disease of widespread economic importance that affects numerous plant species, including Arabidopsis thaliana. We describe a pathosystem between A. thaliana and biovar 3 phylotype I strain BCCF402 of R. solanacearum isolated from Eucalyptus trees. A. thaliana accession Be-0 was susceptible and accession Kil-0 was tolerant. Kil-0 exhibited no wilting symptoms and no significant reduction in fitness (biomass, seed yield, and germination efficiency) after inoculation with R. solanacearum BCCF402, despite high bacterial numbers in planta. This was in contrast to the well-characterized resistance response in the accession Nd-1, which limits bacterial multiplication at early stages of infection and does not wilt. R. solanacearum BCCF402 was highly virulent because the susceptible accession Be-0 was completely wilted after inoculation. Genetic analyses, allelism studies with Nd-1, and RRS1 cleaved amplified polymorphic sequence marker analysis showed that the tolerance phenotype in Kil-0 was dependent upon the resistance gene RRS1. Knockout and complementation studies of the R. solanacearum BCCF402 effector PopP2 confirmed that the tolerance response in Kil-0 was dependent upon the RRS1-PopP2 interaction. Our data indicate that the gene-for-gene interaction between RRS1 and PopP2 can contribute to tolerance, as well as resistance, which makes it a useful model system for evolutionary studies of the arms race between plants and bacterial pathogens. In addition, the results alert biotechnologists to the risk that deployment of RRS1 in transgenic crops may result in persistence of the pathogen in the field.

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Characterization and pathogenicity to geranium of Brazilian strains of Ralstonia spp.

Rossato

Santiago

Mizubuti

et al. 2017

Trop. plant pathol.

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Ralstonia solanacearum (race 3 biovar 2, phylotype IIB, brown-rot ecotype) became an agent of bioterrorism in the USA after the assessment that its introduction in association with geranium plants would affect other crops, especially potatoes. Because race3 biovar 2 is ubiquitous in Brazil and geranium cuttings are exported to many countries, two geranium species were challenged with 32 Brazilian isolates of R. solanacearum and four of R. pseudosolanacearum in order to assess the risk associated with the long-distance spread of the pathogen. Nineteen isolates of nine sequevars of Ralstonia spp. induced wilt in geranium plants, including some belonging to R. pseudosolanacearum (phylotype I), which apparently has not previously been reported infecting this host. No correlations were observed between either race, biovar, phylotype, or sequevar with ability to cause wilt in geranium plants, corroborating the enormous genetic diversity of this species and its isolate-specific virulence. Pelargonium peltatum was more resistant than P. hortorum, indicating that it can be an alternative to reduce crop losses caused by bacterial wilt, even though it also can be a carrier of these pathogens in latent infections.

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Molecular identification of some African strains of Ralstonia solanacearum from eucalypt and potato

Cited by 8 publications

References 15 publications

A Eucalyptus bacterial wilt isolate from South Africa is pathogenic on Arabidopsis and manipulates host defences

A Eucalyptus bacterial wilt isolate from South Africa is pathogenic on Arabidopsis and manipulates host defences

Gene-for-Gene Tolerance to Bacterial Wilt in Arabidopsis

Characterization and pathogenicity to geranium of Brazilian strains of Ralstonia spp.

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